Bgi Shenzhen Co Limited patents

One-stop treatment breaking nucleic acid by means of transposase, and reagent

Disclosed are a one-stop treatment method for breaking a nucleic acid by means of a transposase, and a reagent. The method of the present invention comprises the following steps: conducting random breaking of a nucleic acid by using a transposase-embedded complex, the transposase-embedded complex comprising a transposase and a first... Bgi Shenzhen Co Limited

Method for constructing nucleic acid single-stranded cyclic library and reagents thereof

Provided in the present invention are a method for constructing a nucleic acid single-stranded cyclic library and reagents thereof. The method comprises the steps of using a transposase embedding complex to randomly break nucleic acids and connect a first linker; connecting a second linker at a gap; performing a first... Bgi Shenzhen Co Limited

Mate pair library construction

The present invention provides a novel method for ligating an adapter to a target polynucleotide and methods of generating a library of mate-pair polynucleotide constructs that employ such a ligation method. Libraries and arrays comprising mate-pair polynucleotide constructs, and methods of sequencing libraries and arrays comprising mate-pair polynucleotide constructs, are... Bgi Shenzhen Co Limited

Bubble-shaped adaptor element and constructing sequencing library with bubble-shaped adaptor element

Provided are an adaptor element in a bubble shape, a method of constructing a sequencing library with such an adapter element. The adaptor element is a hybrid formed with a long-chain nucleic acid A and a short-chain nucleic acid B. The hybrid is in the bubble shape with paired regions... Bgi Shenzhen Co Limited

Method and reagent for constructing nucleic acid double-linker single-strand cyclical library

A method and reagent for constructing a nucleic acid double-linker single-strand cyclic library. The method comprises: breaking a nucleic acid into nucleic acid fragments; connecting a first linker sequence; producing by amplification a first product provided with the first linker sequence at either end, where a U nucleobase site is... Bgi Shenzhen Co Limited

Vesicular linker and uses thereof in nucleic acid library construction and sequencing

Provided are a vesicular linker and a single-chain cyclic library constructed by using the linker. The library can be used for RNA sequencing and other sequencing platforms dependent on a single-stranded cyclic library, and has the advantages of high throughput sequencing, high accuracy and simple operations.... Bgi Shenzhen Co Limited

Method and reagent for constructing nucleic acid double-linker single-strand cyclical library

A method and reagent for constructing a nucleic acid double-joint single-strand cyclical library. The method comprises: breaking a nucleic acid into nucleic acid fragments; connecting a first linker sequence; producing by amplification a first product provided with the first linker sequence at either end, where a U nucleobase is provided... Bgi Shenzhen Co Limited

Method of constructing sequencing library

Provided is a method of constructing a sequencing library. The method includes 1) providing a single-stranded DNA fragment from a biological sample; 2) subjecting the single-stranded DNA fragment to whole genomic amplification to obtain a whole genome amplification product; 3) fragmenting the whole genome amplification product using a transposase embedded... Bgi Shenzhen Co Limited

Conotoxin polypeptide k-cptx-bt101, and preparation threof and application thereof

Disclosed are a conotoxin polypeptide κ-CPTx-bt101, a method for preparation thereof, and an application thereof. The conotoxin polypeptide of the present invention consists of 18 amino acids, has a molecular weight of 1872.72 daltons, and has the full sequence KCCTMSVCQPPPVCTCCA (SEQ. ID NO. 1).... Bgi Shenzhen Co Limited

Conotoxin polypeptide k-cptx-bt102, and preparation threof and application thereof

Disclosed are a conotoxin polypeptide κ-CPTx-bt102, a method for preparation thereof, and an application thereof. The conotoxin polypeptide of the present invention consists of 15 amino acids, has a molecular weight of 1660.61 daltons, and has the full sequence RCRCEQTCGTCVPCC (SEQ. ID NO. 1).... Bgi Shenzhen Co Limited

Conotoxin polypeptide k-cptx-bt104, and preparation threof and application thereof

Disclosed are a conotoxin polypeptide κ-CPTx-bt104, a method for preparation thereof, and an application thereof. The conotoxin polypeptide of the present invention consists of 11 amino acids, has a molecular weight of 1313.47 daltons, and has the full sequence SLCCPEDRWCC (SEQ. ID NO. 1).... Bgi Shenzhen Co Limited

Conotoxin polypeptide k-cptx-bt105, and preparation threof and application thereof

Disclosed are a conotoxin polypeptide κ-CPTx-bt105, a method for preparation thereof, and an application thereof. The conotoxin polypeptide of the present invention consists of 16 amino acids, has a molecular weight of 1626.62 daltons, and has the full sequence GICCVDDTCTTHSGCL (SEQ. ID NO. 1).... Bgi Shenzhen Co Limited

Conotoxin polypeptide k-cptx-bt103, and preparation threof and application thereof

Disclosed are a conotoxin polypeptide κ-CPTx-bt103, a method for preparation thereof, and an application thereof. The conotoxin polypeptide of the present invention consists of 29 amino acids, has a molecular weight of 3141.43 daltons, and has the full sequence RTNCGETCLKDEQCVGACQICVPSQLKCL (SEQ ID NO. 1).... Bgi Shenzhen Co Limited

Method for breaking nucleic acid and adding adaptor by means of transposase, and reagent

Provided are a method for breaking a nucleic acid and adding an adaptor by means of a transposase, and a reagent. The method comprises the following steps: conducting random breaking of a nucleic acid by using a transposase-embedded complex, wherein the transposase-embedded complex comprises a transposase and a first adaptor... Bgi Shenzhen Co Limited

Isolated oligonucleotide and use thereof in nucleic acid sequencing

Provided are an isolated oligonucleotide and a use thereof in nucleic acid sequencing, wherein the isolated oligonucleotide comprises a first strand, wherein the 5′-end nucleotide of the first strand has a phosphate group, and the 3′-end nucleotide of the first strand is a dideoxynucleotide, and a second strand, wherein the... Bgi Shenzhen Co Limited

Linker element and using same to construct sequencing library

Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic... Bgi Shenzhen Co Limited

Biomarker compositions specific to coronary heart disease patients and uses thereof

The present invention relates to a disease-specific metabolite profile, and particularly to a biomarker composition obtained by screening from urine-specific metabolite profiles of coronary heart disease subjects. The present invention also relates to a use of the biomarker compositions in risk assessment, diagnosis, early diagnosis, or pathological staging of coronary... Bgi Shenzhen Co Limited

Method and system for screening nanobody

Provided are a method for screening for nanobodies and a corresponding system. The method uses polymerase chain reactions and cDNA 5′ end rapid amplification technology to screen for and obtain nanobodies. The experiment cycle requires only approximately 21 days.... Bgi Shenzhen Co Limited

Biomarker compositions specific to coronary heart disease patients and uses thereof

The present invention relates to a disease-specific metabolite profile, and particularly to a biomarker composition obtained by screening from blood plasma-specific profiles of coronary heart disease subjects. The present invention also relates to a use of the biomarker compositions in risk assessment, diagnosis, early diagnosis, or pathological staging of coronary... Bgi Shenzhen Co Limited