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Life Technologies Corporation patents


Recent patent applications related to Life Technologies Corporation. Life Technologies Corporation is listed as an Agent/Assignee. Note: Life Technologies Corporation may have other listings under different names/spellings. We're not affiliated with Life Technologies Corporation, we're just tracking patents.

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 new patent  Determining the limit of detection of rare targets using digital pcr

A method for determining false positives calls in a biological data plot is provided. The method includes identifying a first data cluster as non-amplification data points within the biological data plot and identifying a second data cluster as wild-type positives within the biological data plot. ... Life Technologies Corporation

 new patent  Pellets used in cell culture and methods of making thereof

The present invention is directed generally to dry cell culture media or feeds in pellet formats which can be reconstituted into liquid media for culturing cells in vitro. Each pellet composition may comprise the same or a different composition; for example, different vitamins, amino acids, buffers, trace salts, ph, iron chelators, etc. ... Life Technologies Corporation

Serum-free suspension system for lentiviral production

A lentiviral vector production system comprises (a) a lentiviral culture supplement to control cell growth, (b) a transfection reagent comprising dhdms, dope, and cholesterol to increase transfection efficiency, (c) a lentiviral production enhancer comprising sodium propionate, sodium butyrate, and caffeine to boost lentiviral production, wherein the lentiviral vector production system is serum-free. A method of lentiviral vector production comprises using the lentiviral production system. ... Life Technologies Corporation

Life Technologies Corporation

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Chemical sensor with consistent sensor surface areas

A chemical sensor is described. The chemical sensor includes a chemically-sensitive field effect transistor including a floating gate conductor having an upper surface. ... Life Technologies Corporation

Filter-based method for efficient capture of lysis of suspended cells

A cell lysis workflow involving a cell suspension that is passed through a filter in a first flow direction from a first side of the filter toward a second side thereof. The filter captures a plurality of cells on the first side. ... Life Technologies Corporation

Devices, systems and methods for illuminating and imaging objects

An illumination system includes a surface configured to have an imaging target placed thereon, a light source, a beam splitter and at least a first mirror. The beam splitter is configured to split the beam of light from the light source and the first mirror is configured to reflect a first beam from the beam splitter onto the surface with the imaging target. ... Life Technologies Corporation

Modified nucleotides and uses thereof

In some embodiments, the disclosure relates generally to methods, as well as related, systems, compositions, kits and apparatuses, for nucleic acid analysis that involve the use of modified nucleotides, including terminator nucleotides and/or tagged nucleotides, in a template-dependent nucleotide incorporation reaction. In some embodiments, the nucleic acid analysis can be conducted at a single reaction site, or at a plurality of reaction sites in an array of reaction sites. ... Life Technologies Corporation

Liquid mixing system with vertically adjustable mixing element and method of use

A liquid mixing system includes a support housing at least partially bounding a compartment. A mount is secured to the support housing. ... Life Technologies Corporation

Chemical sensor with air via

In one embodiment, a chemical sensor is described. The chemical sensor includes a chemically-sensitive field effect transistor including a floating gate conductor having an upper surface, a first opening extending through a first material and through a portion of a second material located on the first material and a second opening extending from the bottom of the first opening to the top of a liner layer located on the upper surface of the floating gate conductor.. ... Life Technologies Corporation

Linking methods, compositions, systems, kits and apparatuses

In some embodiments, the disclosure relates generally to methods as well as related compositions, systems, kits and apparatus comprising linking proteins to target compounds and/or to locations of interest using tethers. For example, the tether can be used to link the protein to a target compound, for example, to link an enzyme to a substrate. ... Life Technologies Corporation

Systems and methods for run-time sequencing run quality monitoring

Systems and methods of providing run-time quality control and monitoring of a single or multiple sequencing runs are provided herein. In some embodiments, the run-time system includes or is in communication with a processor capable of determining various types of run-time information relating to the quality, progress, etc. ... Life Technologies Corporation

Gene fusion

The disclosure provides gene fusion variants and novel associations with disease states, as well as kits, probes, and methods of using the same.. . ... Life Technologies Corporation

Protein detection using fet

Accordingly, in some embodiments methods for detecting an analyte or analytes in one or more sample(s) are provided. The methods encompass providing a solid support with an addressable marker and an associated ligand, contacting the solid substrate to a sample, thereby forming a contacted solid support, associating the contacted solid support with a fet array and detecting the electrical properties of the fet array and thereby detecting an analyte or analytes in one or more samples. ... Life Technologies Corporation

04/12/18 / #20180100191

Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis

Nucleotide triphosphate probes containing a molecular and/or atomic tag on a γ and/or β phosphate group and/or a base moiety having a detectable property are disclosed, and kits and method for using the tagged nucleotides in sequencing reactions and various assay. Also, phosphate and polyphosphate molecular fidelity altering agents are disclosed.. ... Life Technologies Corporation

04/05/18 / #20180094297

Gene expression profiling from ffpe samples

Methods and compositions relating to the generation and use of gene expression data from tissue samples that have been fixed and embedded are provided. The data can electronically stored and implemented as well as used to augment diagnosis and treatment of diseases.. ... Life Technologies Corporation

04/05/18 / #20180094250

Methods and compositions for pcr

A modified thermostable pol b dna polymerase, produced by a reaction, under essentially aqueous conditions, of a thermostable pol b dna polymerase and a modifier reagent of formula i wherein the reaction results in a thermally reversible inactivation of the thermostable pol b dna polymerase activity and the 3′-5′ exonuclease activity, which polymerase is suitable for hot-start pcr. Also disclosed are the method for the modification, a polynucleic acid amplification method and pcr reaction mixture and kit comprising the modified thermostable pol b dna polymerase.. ... Life Technologies Corporation

04/05/18 / #20180092638

System and method for rapid attachment of cardiovascular prosthesis

An embodiment includes a system comprising: first and second conduits respectively including first and second barbed rods; first and second shafts; wherein: (a)(i) in a first configuration the first shaft slides distally within the first conduit and couples to a proximal end of the first barbed rod, and (a)(ii) in a second configuration the first shaft slides distally within the first conduit and pushes the first barbed rod out of the first conduit; wherein: (a)(i) in an additional first configuration the second shaft slides distally within the second conduit and couples to a proximal end of the second barbed rod, and (a)(ii) in an additional second configuration the second shaft slides distally within the second conduit and pushes the second barbed rod out of the second conduit. Other embodiments are described.. ... Life Technologies Corporation

03/29/18 / #20180089366

Systems and methods for mapping sequence reads

Systems, methods, and computer program products for aligning a fragment sequence to a target sequencing. The alignment is allowed at most one gap, such as an insertion or a deletion. ... Life Technologies Corporation

03/29/18 / #20180087056

Oligonucleotide compositions with enhanced efficiency

The oligonucleotide compositions of the present invention make use of combinations of oligonucleotides. In one aspect, the invention features an oligonucleotide composition including at least 2 different oligonucleotides targeted to a target gene. ... Life Technologies Corporation

03/29/18 / #20180087048

Alkylene glycols and polymers and copolymers thereof for direct isolation of nucleic acid from embedded samples

Methods of directly isolating nucleic acid from an embedded biological sample are provided. An emulsified digest is generated in the presence of a thermostable protease, and an additive selected from an alkylene glycol, a poly(alkylene glycol), or a block copolymer having an average mn of 76 to 2900, or a salt or derivative or combination thereof. ... Life Technologies Corporation

03/22/18 / #20180078915

Bioreactor with impeller assembly

A bioreactor includes a flexible container and an impeller assembly having a hollow tube. The impeller assembly is coupled to the flexible container. ... Life Technologies Corporation

03/22/18 / #20180078662

Methods and compositions for enzyme-mediated site-specific radiolabeling of glycoproteins

Provided herein are methods, compositions and kits for use in the site-specific labeling of glycoproteins comprising a combination of enzyme-mediated incorporation of modified sugars comprising a chemical handle and cycloaddition chemistry with a labeling molecule comprising a reactive group, a metal ion chelator, and/or a fluorophore.. . ... Life Technologies Corporation

03/15/18 / #20180073055

Nucleic acid extraction and amplification controls and methods of use thereof

Nucleic acid reagents and corresponding methods of using the same for monitoring and evaluating nucleic acid extraction and amplification reactions.. . ... Life Technologies Corporation

03/15/18 / #20180073009

Compositions and methods for preparing short rna molecules and other nucleic acids

The invention provides methods of preparing nucleic acids, such as rna molecules, of a defined size or range of sizes. The invention provides compositions, methods and kits for use in the production and preparation of small rna molecules (including without limitation micro-rna, sirna, d-sirna and e-sirna) and other nucleic acids of various sizes.. ... Life Technologies Corporation

03/15/18 / #20180071700

Fluid mixing system with flexible drive line and foldable impeller

A fluid mixing system includes a container, such as a flexible bag, bounding a compartment. A flexible drive line is disposed within the compartment, the drive line having a first end rotatably connected to a first end of the container and an opposing second end rotatably connected to a second end of the container. ... Life Technologies Corporation

03/08/18 / #20180068061

Systems and methods for detecting homopolymer insertions/deletions

Systems and method for determining variants can receive mapped reads and determine a distribution of matched-filter residuals distribution from a plurality of reads at a homopolymer region. The distribution of matched-filter residuals can be fit to uni-modal and bi-modal models. ... Life Technologies Corporation

03/08/18 / #20180067044

Apparatuses, systems, methods, and computer readable media for acoustic flow cytometry

A flow cytometer includes a capillary having a sample channel; at least one vibration producing transducer coupled to the capillary, the at least one vibration producing transducer being configured to produce an acoustic signal inducing acoustic radiation pressure within the sample channel to acoustically concentrate particles flowing within a fluid sample stream in the sample channel; and an interrogation source having a violet laser and a blue laser, the violet and blue lasers being configured to interact with at least some of the acoustically concentrated particles to produce an output signal.. . ... Life Technologies Corporation

03/08/18 / #20180067037

Systems, methods, and apparatuses for optical systems in flow cytometers

The present set of embodiments relate to a system, method, and apparatus for an optical configuration in a flow cytometer that allows for independent adjustment of focusing for each light source. Such systems, methods, and apparatuses require a final focusing element to be moved near the beginning of the optical train and for each optical element coming after the final focusing element to be configured to accommodate converging light beams while minimizing the introduction of aberrations into those beams.. ... Life Technologies Corporation

03/01/18 / #20180060481

Identification, monitoring and treatment of infectious disease and characterization of inflammatory conditions related to infectious disease using gene expression profiles

A method is provided in various embodiments for determining a profile data set for a subject with infectious disease or inflammatory conditions related to infectious disease based on a sample from the subject, wherein the sample provides a source of rnas. The method includes using amplification for measuring the amount of rna corresponding to at least 2 constituents from table 1. ... Life Technologies Corporation

03/01/18 / #20180057814

Compositions and methods for inhibition of nucleic acids function

The invention relates generally to compositions and methods for inhibiting the function of target nucleic acids by sequence specific binding. The compositions and methods can be used for inhibition of micro rnas and other relatively short non-coding rnas.. ... Life Technologies Corporation

03/01/18 / #20180056296

Apparatuses, systems and methods for providing thermocycler thermal uniformity

A thermal block assembly including a sample block and two or more thermoelectric devices, is disclosed. The sample block has a top surface configured to receive a plurality of reaction vessels and an opposing bottom surface. ... Life Technologies Corporation

02/15/18 / #20180044371

Methods for isolation, identification, and quantification of mirnas

Method and compositions and kits for isolation, identification, and quantification of mirnas and other small rnas, including but not limited to, sirnas, mrnas, and snrnas are disclosed. Methods of diagnosing a disease or its progression are also disclosed.. ... Life Technologies Corporation

02/08/18 / #20180037931

Chemically-enhanced primer compositions, methods and kits

A chemically-enhanced primer is provided comprising a negatively charged moiety (ncm), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. ... Life Technologies Corporation

02/01/18 / #20180031545

Charged reactive oligomers

Methods, systems, compositions and kits are described for detecting cleaved glycans from a glycoconjugate. After labeling the glycan with a nucleic acid charged oligomer described herein, the labeled glycans can be separated under the influence of an electric field or based on their detectable tag and identified.. ... Life Technologies Corporation

02/01/18 / #20180031522

Sharply resolving labeled protein molecular weight standards

Pre-labeled protein standards useful in electrophoresis that have sharp, consistent separation characteristics that are substantially the same as those of their unlabeled counterparts are provided. The invention provides pre-labeled protein standard sets that include a plurality of labeled proteins that are labeled on a first amino acid, in which side reactions of the label with amino acids not targeted for labeling are reduced.. ... Life Technologies Corporation

02/01/18 / #20180030534

Bi-directional sequencing compositions and methods

In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, in some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.. . ... Life Technologies Corporation

01/25/18 / #20180024094

Electrophoresis gel cassette and comb

Provided herein is an apparatus for gel electrophoresis comprising a cassette and a comb having at least one wedge-shaped tooth.. . ... Life Technologies Corporation

01/25/18 / #20180023075

Compositions and methods for enhancing homologous recombination

The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.. ... Life Technologies Corporation

01/18/18 / #20180018422

Systems and methods for nucleic acid-based identification

Systems and methods for calculating a predictive index of identity of a nucleic acid sample using polymorphic genetic marker data are provided. In one embodiment, a predictive index of identity of the nucleic acid sample is calculated using a value from a second set of data from a polymorphic genetic marker that is not linked to a polymorphic genetic marker used to produce a first set of data. ... Life Technologies Corporation

01/18/18 / #20180017523

Electro-blotting devices, systems, and kits and methods for their use

The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). ... Life Technologies Corporation

01/18/18 / #20180016572

Compositions and methods for detecting nucleic acid regions

Provided are methods, compositions, reagents, kits that are useful for detecting a specific nucleic acid region in genome with high efficiency and high sensitivity.. . ... Life Technologies Corporation

01/11/18 / #20180010177

Enhanced ligation reactions

In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. ... Life Technologies Corporation

01/11/18 / #20180010105

Novel reverse transcriptases for use in high temperature nucleic acid synthesis

The invention provides novel reverse transcriptases (rts) with desirable properties such as increased thermostability, increased thermoreactivity and/or increased resistance to inhibitors. In certain embodiments, the invention provides methods of producing, amplifying and/or sequencing nucleic acid molecules (particularly cdna molecules) using kits, compositions and/or reactions mixtures containing such novel reverse transcriptase enzymes.. ... Life Technologies Corporation

01/11/18 / #20180008410

Heart valve sewing cuff

An embodiment of the invention includes a sewing cuff for aortic heart valves that better approximates native anatomy by better mating with the crown-like anatomical annulus. Limiting distortion of the crown-like annulus provides better blood flow and overall valve function and provides a physician greater ease of implantation since native anatomy is not flattened. ... Life Technologies Corporation

01/04/18 / #20180003672

Method for treating a semiconductor device

A sensor array includes a plurality of sensors. A sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array. ... Life Technologies Corporation

12/28/17 / #20170369857

Nucleotide transient binding for sequencing methods

Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (snp) analyses. ... Life Technologies Corporation

12/28/17 / #20170369431

Hydrazinyl and aminooxy compounds and their methods of use

The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.. ... Life Technologies Corporation

12/21/17 / #20170362640

Novel compositions, methods and kits for microorganism detection

Methods, compositions and kits for detecting microorganisms and/or profiling microbiota such as for example through use of nucleic acid amplification and detection.. . ... Life Technologies Corporation

12/21/17 / #20170362624

Labeling and detection of post translationally modified proteins

Provided in certain embodiments are new methods for forming azido modified biomolecule conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling a biomolecules with an azide group.. ... Life Technologies Corporation

12/14/17 / #20170354901

Acoustically settled liquid-liquid sample purification system

A sample purification system includes a container assembly bounding a sample purification compartment and having an upper end and an opposing lower end, the sample purification compartment comprising mixing zones and settling zones. A plurality of shielding elements are positioned within the sample purification compartment so as to at least partially separate adjacent mixing zones and settling zones or separate adjacent mixing zones, the mixing zones being in fluid communication with the settling zones. ... Life Technologies Corporation

12/07/17 / #20170351807

Methods and systems for designing gene panels

A system and method of selecting genes for a gene panel, includes retrieving gene-disease associations of genes associated with diseases at a given level in the disease hierarchy from a disease association database. The disease association database stores disease information, gene information, phenotype information, associations between diseases in the disease hierarchy, gene-disease associations and strength parameters related to the gene-disease associations. ... Life Technologies Corporation

11/30/17 / #20170342371

Method of culturing a cell with a supplement

Cell culture media, concentrated media and feeds, methods of manufacturing cell culture media and feeds, and methods of culturing cells are provided. One or more small peptides, including dipeptides are added to the cell culture media to provide improved stability and improved conditions for culturing cells.. ... Life Technologies Corporation

11/23/17 / #20170337507

Inventory management system and method of use

Provided is a computer-implemented method for product management the method can include obtaining user identification information from a user, wherein the identification code is associated with a financial account, of the user; validating, by a processor, that the identification information is authentic based on registration information that was previously received; providing first access signal to a locking mechanism of a locking enclosure to unlock the locking mechanism based on the validating, wherein the locking enclosure securely stores a purchasable product; obtaining product identification information from a scan of a bar code associated with the product providing the product information that was obtained to be rendered on a display; receiving an indication from the user that the product information is correct; and providing a second access signal to the locking mechanism to lock the locking mechanism based on the indication that was received.. . ... Life Technologies Corporation

11/23/17 / #20170335387

Systems and methods for identifying sequence variation

Systems and method for determining variants can receive mapped reads, align flow space information to a flow space representation of a corresponding portion of the reference. Reads spanning a position with a potential variant can be evaluated in a context specific manner. ... Life Technologies Corporation

11/23/17 / #20170335377

Se33 mutations impacting genotype concordance

Disclosed are primer set compositions, methods and kits for human identification using the highly complex sequence locus, se33 (actbp2) in single and multiplex pcr reactions. Additionally, disclosed are three newly discovered single nucleotide polymorphisms (snps) within the se33 locus that can cause discordance seen as mobility shift or allelic dropout. ... Life Technologies Corporation

11/23/17 / #20170333457

Anti-viral azide containing compounds

Methods of using azide-modified biomolecules, such as fatty acids, carbohydrates and lipids, to treat a plant, an insect or an animal infected with a virus or to inhibit infectivity of a virus, such as the human immunodeficiency virus, are provided. Also provided are methods of labeling a virus, such as human immunodeficiency virus, with an azide-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. ... Life Technologies Corporation

11/16/17 / #20170330327

Systems, methods, and apparatuses for image capture and display

Provided are systems and methods that allow a user to capture images at low- and high-level magnification and then overlay the high-level magnification images on the low-level magnification image to ease review of the images. The high-level magnification images may be overlaid on the low-level magnification image based at least in part on the portion of the low-level magnification image from which the high-level image was originated.. ... Life Technologies Corporation

11/16/17 / #20170328862

Expanding cam lock for sealing slab gels in an electrophoresis apparatus

An expanding cam lock for use with an electrophoresis system is disclosed herein. The cam lock allows the simultaneous use of multiple slab gel cassettes in first and second buffer core assemblies in an electrophoresis system while maintaining the necessary compressive force to create a liquid-tight seal between the anode and cathode buffer solutions. ... Life Technologies Corporation

11/16/17 / #20170327902

Penta e polymorphisms for human identification

Disclosed herein are methods and compositions for human identification using polymorphisms in the penta e short tandem repeat locus. These newly disclosed polymorphisms are significant in preventing allelic drop out.. ... Life Technologies Corporation

11/09/17 / #20170321271

Combinatorial barcode sequences, and related systems and methods

A kit for use with a nucleic acid sequencing instrument can include a plurality of combinatorial barcodes sequences meeting the following criteria: each of the combinatorial barcode sequences comprise a plurality of iterations of a sequence motif, where the sequence motif comprises a first nucleotide base from a first group of nucleotide bases followed by a second nucleotide base from a second group of nucleotide bases, the first group and the second group differing from each other; and the plurality of combinatorial barcode sequences is at least 1,000,000 different barcode sequences.. . ... Life Technologies Corporation

11/09/17 / #20170321269

Method of determining the nucleotide sequence of oligonucleotides and dna molecules

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of dna polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a dna fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. ... Life Technologies Corporation

11/09/17 / #20170321245

Flash and glow 1,2-dioxetanes

Compounds having chemiluminescent flash and glow properties. Also disclosed are methods using the compounds to generate light, detect and/or quantify enzymes, antigens, and/or nucleic acids. ... Life Technologies Corporation

11/09/17 / #20170321219

Oligonucleotide compositions with enhanced efficiency

The oligonucleotide compositions of the present invention make use of combinations of oligonucleotides. In one aspect, the invention features an oligonucleotide composition including at least 2 different oligonucleotides targeted to a target gene. ... Life Technologies Corporation

10/26/17 / #20170307529

Optical systems and methods for biological analysis

An instrument for processing and/or measuring a biological process contains an excitation source, a sample holder, an optical sensor, an excitation optical system, and an emission optical system. The sample holder is configured to receive a plurality of biological samples. ... Life Technologies Corporation

10/26/17 / #20170307447

Probe port and realted container system

A container system includes a flexible bag, a probe port, and a probe. The probe port includes an elongated tubular member having an interior surface bounding a first passageway extending between a first end and an opposing second end, the tubular member being made of a polymeric or elastomeric material and being flexible. ... Life Technologies Corporation

10/26/17 / #20170306306

Compositions and methods for enhancing homologous recombination

The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.. ... Life Technologies Corporation

10/26/17 / #20170306072

Particles containing multi-block polymers

Polymer particles having a multi-block vinylic polymer attached to their surface are disclosed. The particles can be used in a variety of purification and detection methods.. ... Life Technologies Corporation

10/26/17 / #20170305954

Synthesis of 2', 3'-dideoxynucleosides for automated dna synthesis and pyrophosphorolysis activated polymerization

Methods for preparation of 2′,3′-dideoxynucleotides support structures, such as 2′,3′-dideoxyguanosine, 2′,3′-dideoxyadenosine, and 3′-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated dna synthesis and pyrophosphorolysis activated polymerization.. ... Life Technologies Corporation

10/12/17 / #20170293713

Models for analyzing data from sequencing-by-synthesis operations

A method of modeling a background signal when sequencing a polynucleotide strand using sequencing-by-synthesis includes: flowing a series of nucleotide flows onto a reactor array having multiple reaction confinement regions, one or more copies of the polynucleotide strand being located in a loaded reaction confinement region of the reactor array, the loaded reaction confinement region being located in a vicinity of one or more neighboring reaction confinement regions that may or may not be loaded; receiving output signals from the reactor array; and modeling a background signal for the loaded reaction confinement region using the received output signals and a model adapted to account at least for an exchange of ions between the one or more neighboring reaction confinement regions and a headspace adjacent the loaded reaction confinement region and the one or more neighboring reaction confinement regions.. . ... Life Technologies Corporation

10/12/17 / #20170292156

Modified proteins and methods of making and using same

Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pka values are described. ... Life Technologies Corporation

10/12/17 / #20170292144

Compositions, methods, and kits for synthesis and detection of nucleic acids

Compositions, methods, and kits for synthesizing, detecting, and/or quantifying nucleic acids are provided herein. Embodiments comprise a nucleic acid amplification composition comprising a thermostable dna polymerase and agents which improve nucleic acid synthesis, amplification, detection, and/or quantification of nucleic acid targets in a crude extract or crude lysate sample.. ... Life Technologies Corporation

10/05/17 / #20170284907

Automated microdissection instrument

Systems and methods for automated laser microdissection are disclosed including automatic slide detection, position detection of cutting and capture lasers, focus optimization for cutting and capture lasers, energy and duration optimization for cutting and capture lasers, inspection and second phase capture and/or ablation in a quality control station and tracking information for linking substrate carrier or output microdissected regions with input sample or slide.. . ... Life Technologies Corporation

10/05/17 / #20170283868

Methods and apparatus for single molecule sequencing using energy transfer detection

Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. ... Life Technologies Corporation

09/28/17 / #20170275688

Methods, compositions and systems for sample deposition

Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. ... Life Technologies Corporation

09/28/17 / #20170275674

Next-generation sequencing quality control monitoring tool

Systems and methods are used to provide an online tool for clinical laboratories to monitor next-generation sequencing (ngs) workflow using quality control (qc) material that can be used for multiple assays. The tool utilizes a highly multiplexed qc with ngs assays that detect somatic mutations. ... Life Technologies Corporation

09/21/17 / #20170269109

Method and apparatus for automated sample manipulation

The present teachings provide apparatuses and methods for automated handling of samples, e.g., biological or chemical samples. The apparatuses and the methods of the present teachings allow automated performance of various sample manipulation steps without manual intervention. ... Life Technologies Corporation

09/14/17 / #20170260512

Polymerase compositions, methods of making and using same

The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. ... Life Technologies Corporation

09/07/17 / #20170254775

Isotopically-labeled proteome standards

The invention provides methods for quantifying biomolecules, such as polypeptides in mass spectrometric analysis. The methods include use of a biomolecule standard having at least one atomic isotope different than that of the naturally occurring isotopes in the biomolecule of interest. ... Life Technologies Corporation

09/07/17 / #20170253922

Human identification using a panel of snps

The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex pcr of one or more nucleic acids which belong to a panel of single nucleotide polymorphisms (snps) useful to identify a human. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences in the panel. ... Life Technologies Corporation

09/07/17 / #20170253921

Methods, kits & compositions for determining gene copy numbers

The present specification relates to methods, compositions, and kits for determining the presence of the number of copies of a gene. The present disclosure provides methods and compositions to determine zygosity in transgenic plants, number of transgenes in a plant or animal, karyotyping, determine cnv in human and animal samples, diagnose and detect diseases and conditions that are characterized by having variations in the number of copies of one or more genes.. ... Life Technologies Corporation

08/31/17 / #20170248606

Apparatuses, methods, computer program products, and kits for hi-throughput glycan analysis

An apparatus for glycan analysis is disclosed. The apparatus includes a plurality of loading wells adapted to receive a plurality of samples; a plurality of capillaries arranged in correspondence with the loading wells, each of the capillaries including a first portion including a stacking gel and a second portion including a resolving gel; and a plurality of eluting wells arranged in correspondence with the capillaries and adapted to receive a portion of the samples having traversed the capillaries.. ... Life Technologies Corporation

08/31/17 / #20170247756

Genetic sequence verification compositions, methods and kits

Methods, compositions and kits are described for resequencing, confirming or verifying next generation sequencing (ngs) results with sanger sequencing. These methods are particularly useful for samples having very limited quantities such as formalin-fixed, paraffin-embedded (ffpe), laser capture microdissection (lcm), fine needle biopsies or aspirates.. ... Life Technologies Corporation

08/31/17 / #20170247504

Dibenzosilole monomers and polymers and methods for their preparation and use

Water-soluble, conjugated polymers containing one or more dibenzosilole monomer residues, as well as compositions, kits, and methods of making and using such polymers are disclosed. Also disclosed are dibenzosilole derivatives substituted with one or more water-solubilizing groups, and methods of making and using such derivatives to prepare water-soluble dibenzosilole polymers.. ... Life Technologies Corporation

08/31/17 / #20170246603

Gas spargers and related container systems

A container system includes a collapsible bag having an interior surface at least partially bounding a chamber, the chamber being adapted to hold a fluid. A sparger is disposed within the chamber of the bag, the sparger bounding a compartment and having an inside edge that encircles an opening passing through the sparger. ... Life Technologies Corporation

08/24/17 / #20170241943

Methods for calibrating an array of chemically-sensitive sensors

Methods and apparatus relating to very large scale fet arrays for analyte measurements. Chemfet (e.g., isfet) arrays may be fabricated using conventional cmos processing techniques based on improved fet pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. ... Life Technologies Corporation

08/17/17 / #20170235874

Methods and systems for detecting minor variants in a sample of genetic material

A computer-implemented method for determining minor variants. The method includes receiving electropherogram sequence data from a test sample, identifying any non-primary peaks in the electropherogram, and characterizing identified non-primary peaks using at least one signal feature. ... Life Technologies Corporation

08/10/17 / #20170225169

Thermal uniformity for thermal cycler instrumentation using dynamic control

A method for performing polymerase chain reactions (pcr) for improving thermal non-uniformity is provided. The method includes measuring a first temperature, by a first sensor, of a first sample block sector of a sample block and measuring a second temperature, by a second sensor, of a second sample block sector of the sample block that is adjacent to the first sample block sector. ... Life Technologies Corporation

08/03/17 / #20170218095

Monodisperse submicron polymer particles

This invention relates to monodisperse cross-linked polymer particles, comprising particles with a substantially smooth outer surface and an average diameter of less than 1 μm, wherein the particles are solid or porous, and wherein the coefficient of variation (cv) % of the particles, when measured by cps disk centrifugation analysis, is less than 15%. These monodisperse cross-linked polymer particles may comprise magnetic material and are useful in various application. ... Life Technologies Corporation

07/20/17 / #20170206313

Using flow space alignment to distinguish duplicate reads

Systems and method for identifying duplicate reads can receive first and second reads, determine if the first and second reads have a same start and end position, determine a binary flow difference, and identify the second read as a duplicate of the first read when the binary flow difference exceeds a threshold.. . ... Life Technologies Corporation

07/20/17 / #20170204398

Nucleic acid preparation method

A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support whilst maintaining it on the support. ... Life Technologies Corporation

07/13/17 / #20170199734

Systems and methods for versioning hosted software

Systems and methods for performing a validated analysis can include access to first and second versions of an analysis engine. The first version of the analysis engine can be used to perform a previously validated analysis. ... Life Technologies Corporation

07/13/17 / #20170197185

Container with film sparger

A container assembly includes a flexible bag having an interior surface bounding a chamber and an opposing exterior surface, the bag having a bottom end wall being comprised of a first sheet overlying a second sheet, the first sheet and the second sheet of the bottom end wall being welded together so as to form a first sparging area and a separated second sparging area that are both bounded between the first sheet and the second sheet, at least a portion of the first sheet overlying the first and second sparging areas being gas permeable.. . ... Life Technologies Corporation

07/06/17 / #20170194985

Methods and computer program products for compression of sequencing data

A compression method includes measuring a waveform associated with a chemical event occurring on a sensor array, wherein the waveform comprises at least one region associated with expected measured values and at least one region associated with unpredictable measured values; applying a first compression process to the waveform, the first compression process including an averaging of one or more frames in one or more portions of the waveform; applying a keyframe delta compression to the frame-averaged data; forming a compressed data structure including a keyframe and calculated differences subsequent to the keyframe; and storing one or more compressed data structures in memory.. . ... Life Technologies Corporation

07/06/17 / #20170191922

System and method for providing stable fluid flow

An embodiment of a system with a minute measure of pulsatility in a flow of a fluid is described that comprises a first pump configured to flow the fluid to a junction at a first flow rate that comprises a measure of pulsatility; and a second pump configured to flow a portion of the fluid from the junction at a second flow rate that is less than the first flow rate to produce a flow of the fluid at a third flow rate from the junction with a minute measure of pulsatility.. . ... Life Technologies Corporation

07/06/17 / #20170191571

Pinch flow regulator

A valve for regulating fluid flow includes a housing base defining a lower cavity and comprising a pinch structure within the lower cavity, a gas inlet providing external access to the lower cavity, a base fluid inlet, and a base fluid outlet. A housing cover defines an upper cavity and comprises a cover fluid inlet and a cover fluid outlet. ... Life Technologies Corporation

07/06/17 / #20170191051

Labeled enzyme compositions, methods and systems

Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are labeled polymerase conjugates including a polymerase linked to one or more labels, wherein the conjugate has polymerase activity. ... Life Technologies Corporation

06/29/17 / #20170186840

Nanocrystals with high extinction coefficients and methods of making and using such nanocrystals

A population of bright and stable nanocrystals is provided. The nanocrystals include a semiconductor core and a thick semiconductor shell and can exhibit high extinction coefficients, high quantum yields, and limited or no detectable blinking.. ... Life Technologies Corporation

06/29/17 / #20170183617

Fluid mixing system with laterally displaced flexible drive lines and methods of use

A fluid mixing system includes a container bounding a compartment and extending between a first end and an opposing second end. An elongated first drive line and second drive line are disposed within the compartment of the container and are rotatable therein. ... Life Technologies Corporation

06/29/17 / #20170182496

Systems and methods for transfer of liquid samples

A system for preparing biological sample contains a body including a proximal side and a distal side, a plurality of mandrels, a plurality of resilient elements, a plurality of fluid dispensers, and one or more samples. The mandrels are moveably positioned within the body, where each resilient element engages a respective one of the mandrels. ... Life Technologies Corporation

06/29/17 / #20170182492

Integrated system for nucleic acid amplification and detection

Devices, systems, methods, and kits are disclosed for amplifying small quantities of nucleic acid and identifying the nucleic acid sequences of the amplified nucleic acid products.. . ... Life Technologies Corporation

06/22/17 / #20170177792

Methods and systems for knowledge discovery using biological data

A system for analyzing biological data, comprising: a storage configured to store a plurality of data files containing biological data obtained from a plurality of devices; a server configured to: host a plurality of applications, each configured to be implemented on the server and to provide analysis, manipulation, comparison, visualization, or a combination thereof, of the biological data included in the data files, wherein the plurality of applications allow a user to analyze different data files related to the same sample and compare the results of the analysis.. . ... Life Technologies Corporation

06/22/17 / #20170177791

Methods and systems for pcr quantitation

A method for quantifying nucleic acid is provided. The method includes determining a first reference threshold cycle for a first predetermined input quantity for a reference nucleic acid, determining a first target threshold cycle for the first predetermined input quantity for a target nucleic acid, determining a second reference threshold cycle for a second predetermined input quantity for the reference nucleic acid, and determining a second target threshold cycle, by the processor, for the second predetermined input quantity for the target nucleic acid. ... Life Technologies Corporation

06/22/17 / #20170176385

Capillary array cartridge for capillary electrophoresis systems

The present disclosure relates, in some embodiments, to an apparatus for conducting a capillary electrophoresis assay. The apparatus can comprise a capillary array comprising an anode end and a cathode end, the capillary array provided in a housing further comprising a reservoir configured to house a separation medium and an anode buffer. ... Life Technologies Corporation

06/22/17 / #20170176384

Apparatuses, systems and methods for sequencing using capillary electrophoresis

In one aspect, a biological sequencing device comprising a cartridge configured to be removed from the instrument is disclosed. In various embodiments the cartridge can include one or more capillaries suitable for capillary electrophoresis, a reservoir and a pump. ... Life Technologies Corporation

06/22/17 / #20170175171

Methods and kits for breaking emulsions

The disclosure relates generally to methods, kits and systems for extracting biomolecules from a water-in-oil emulsion, including breaking a water-in-oil emulsion comprising a plurality of aqueous droplets in a continuous hydrophobic fraction using a breaking solution to produce a resulting reaction mixture containing one or more biomolecules and manipulating the resulting reaction mixture to form at least two phases, where one of the phases includes an aqueous phase containing the one or more biomolecules.. . ... Life Technologies Corporation

06/22/17 / #20170175092

Mutant rb69 dna polymerase

Provided herein are mutant dna-dependent polymerases which are derived from, or otherwise related to, wild type rb69 dna polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. ... Life Technologies Corporation

06/22/17 / #20170174846

Purification systems and methods

A method of preparing hydrogel particles includes applying a solution including a plurality of hydrogel particles to a stir cell. A retentate side of a filter defines a lower surface of the stir cell. ... Life Technologies Corporation

06/22/17 / #20170174700

Biotin derivatives

Biotin derivatives, methods of using the biotin derivatives and kits comprising the biotin derivatives.. . ... Life Technologies Corporation

06/22/17 / #20170173587

Systems and methods for a thermal cycler heated cover

A thermal cycler system for use with a sample holder configured to receive a plurality of samples includes a sample block configured to receive the sample holder, a cover lid configured to move in a direction toward the sample block from an open position to a closed position, a heated cover operatively coupled to the cover lid and configured to move in a direction toward the sample block from a raised position to a first lowered position, in which the heated cover contacts the sample holder when the sample holder is received by the sample block, and a drive assembly including a motion guide configured to move in a direction toward the sample block from a first position, wherein the cover lid is in the open position and the heated cover is in the raised position, to a second position, wherein the cover lid is in the closed position.. . ... Life Technologies Corporation

06/22/17 / #20170173586

Thermal cycler systems and methods of use

A thermal cycler system for use with a sample holder configured to receive a plurality of samples includes a sample block having an upstanding peripheral side wall and being configured to receive the sample holder and an adaptor having an upstanding peripheral side wall configured to be positioned about the peripheral side wall of the sample block. When the peripheral side wall of the adaptor is positioned about the peripheral side wall of the sample block and the sample holder is received in the sample block, the peripheral side wall of the adaptor extends in an upward direction toward the sample holder.. ... Life Technologies Corporation

06/15/17 / #20170166943

Oligosaccharide modification and labeling of proteins

The present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified oligosaccharides. The invention further relates to kits that may be used to produce the antibodies of the invention.. ... Life Technologies Corporation

06/15/17 / #20170166854

Pipette tip for electroporation device

Provided herein is a pipette tip for electroporation including an outer surface, a void located within the outer surface, and a conductor located at least partially on or within the outer surface, in electrical communication with at least a portion of the outer surface and the void. Further provided herein is a pipette tip for electroporation including a body, a connector, and an elongated part. ... Life Technologies Corporation

06/15/17 / #20170166603

Large stokes shift dyes

Provided herein are heptamethine cyanine dyes having a large stokes shift, and the salts and conjugates thereof. Also provided are methods of using and making such large stokes shift dyes as fluorescence resonance energy transfer (fret) acceptors or donors.. ... Life Technologies Corporation

06/08/17 / #20170161467

Apparatus for collecting fingerprints and buccal swabs

Disclosed are devices and methods for collection, labeling and matching biological samples containing nucleic acid in conjunction with collecting at least one ridge and valley signature such as a fingerprint or footprint of an individual. Such devices and methods are used in forensic, human identification, paternity, tissue typing, and screening technologies to rapidly process an individual's identity, determine the identity of an individual along with the genotype profile of the individual.. ... Life Technologies Corporation

06/08/17 / #20170161429

Methods and systems for streamlining optical calibration

A method for calibrating a biological instrument is provided. The method comprises the steps of acquiring an image of at least one biological sample array, determining a first region of interest within the image, wherein the first region of interest comprises a first plurality of locations on the at least one biological array; and identifying within the first region of interest, a plurality of image elements associated with each of the first plurality of locations on the at least one biological array.. ... Life Technologies Corporation

06/08/17 / #20170160294

Methods of reversibly binding a biotin compound to a support

Methods of reversal of the binding between a biotin compound and a biotin-binding compound are disclosed. A method of reversibly releasing a biotinylated moiety from a streptavidin (or avidin) coated support is shown as an example. ... Life Technologies Corporation

06/08/17 / #20170160284

Protein melt analysis using dipyrrometheneboron difluoride compounds

According to the present teachings, systems, compositions, kits and methods for protein melt analysis are provided that utilizing a dye that is a dipyrrometheneboron difluoride compound. In some embodiments, a method comprises preparing a sample by mixing at least one protein with a dye, and applying a controlled heating, while recording the fluorescence emission of the sample.. ... Life Technologies Corporation

06/08/17 / #20170160230

Expanding cam lock for sealing slab gels in an electrophoresis apparatus

An expanding cam lock for use with an electrophoresis system is disclosed herein. The cam lock allows the simultaneous use of multiple slab gel cassettes in first and second buffer core assemblies in an electrophoresis system while maintaining the necessary compressive force to create a liquid-tight seal between the anode and cathode buffer solutions. ... Life Technologies Corporation

06/08/17 / #20170157538

System and method for emulsion breaking

A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.. . ... Life Technologies Corporation

06/01/17 / #20170153204

Preparation of electrophoresis gels, and related devices, systems, and methods

An apparatus for preparation of an electrophoresis slab gel may include a base having an opening configured to receive a cassette configured to contain an electrophoresis slab gel, a clamping mechanism configured to move relative to the base between an open position in which the clamping mechanism permits insertion of a cassette into the base, and a closed position in which the clamping mechanism is configured to clamp a cassette received in the base, a compressible pad operatively coupled to the clamping mechanism in a position to compress against a cassette received in the base in the closed position of the clamping mechanism. The cassette may include a first plate and a second plate, and a spacer mechanism separate from each of the first and second plates, the spacer mechanism configured to be positioned between the inner faces of the first plate and the second plate. ... Life Technologies Corporation

06/01/17 / #20170153201

Chemical sensor with conductive cup-shaped sensor surface

A system includes a sensor including a sensor pad and a well wall structure defining a well operatively coupled to the sensor pad. The well is further defined by a lower surface disposed over the sensor pad. ... Life Technologies Corporation

06/01/17 / #20170152469

Reactor foam sensor systems and methods of use

A foam sensor system includes a container bounding a compartment. A foam sensor assembly is mounted on the container and includes: a base being secured to the container; a foam contact being spaced apart from the base and disposed within the compartment of the container, at least a portion of the foam contact having a first diameter; and a transition member extending between the base and the foam contact with at least a portion of the transition member being openly exposed within the compartment of the container, at least a portion of the transition member having a second diameter that is smaller than the first diameter, the foam contact and transition member being connected together so that an electrical signal can pass therethrough.. ... Life Technologies Corporation

05/18/17 / #chemiluminescent compositions, methods, assays and kits for oxidative enzymes are described. further disclosed are dioxetane compounds of the form:

Life Technologies Corporation

. . ... Life Technologies Corporation

05/18/17 / #20170140671

Surgery simulation system and method

A surgery simulation system and method is disclosed. The surgery simulation system includes a simulation platform provided with a physical model thereon for simulating a human organ for incising and/or piercing; a force feedback device provided with an actuator and a surgical instrument connection component thereon, the surgical instrument connection component being detachably connected to a surgical instrument; a three-dimensional tracker for tracking the surgical instrument; a display device for displaying a virtual organ and/or a medical image; a control module having an electrical signal connection with the force feedback device, the three-dimensional tracker and the display device, controlling the force feedback device according to the signal of the three-dimensional tracker, and displaying a corresponding image on the display device. ... Life Technologies Corporation

05/18/17 / #20170140093

Methods and systems for in silico design

Embodiments describe computer systems and computer programs for implementing biocad methods comprising one or more data models and one or more biocad tools, wherein the biocad tools enable users to design or refactor a biomolecule or to conduct a biological experiment in silico by user input of one or more components selected by the user from a database populated with information on components and scientific data of existing biomolecules and experiments. Data models of the programs are operable to manage development of the new biomolecule or experiment based on information in the databases. ... Life Technologies Corporation

05/18/17 / #20170137866

Methods and compositions for labeling nucleic acids

The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. ... Life Technologies Corporation

05/18/17 / #20170137816

Double-stranded oligonucleotides

Antisense sequences, including duplex rnai compositions, which possess improved properties over those taught in the prior art are disclosed. The invention provides optimized antisense oligomer compositions and method for making and using the both in in vitro systems and therapeutically. ... Life Technologies Corporation

05/11/17 / #20170132359

Systems and methods for identifying somatic mutations

Systems and method for identifying somatic mutations can receive first and second sequence information, determine if a variant present in the first sequencing information is also present in the second sequence information, and identify variants present in the first sequence information are somatic mutations when the variant is either not present in the second sequence information or the presence of the variant in the second sequence information is likely due to a sequencing error.. . ... Life Technologies Corporation

05/11/17 / #20170131316

Sampling probes, systems, apparatuses, and methods

A sampling system is provided. The sampling system includes a housing. ... Life Technologies Corporation

05/11/17 / #20170130264

Novel compositions, methods and kits for enhancing pcr specificity

The present disclosure provides novel primers and method for the detection of specific nucleic acid sequences. The primers and methods provided herein are useful in a wide variety of molecular biology applications and are particularly useful in allele-specific pcr.. ... Life Technologies Corporation

05/11/17 / #20170128515

Antimicrobial & antiviral composition

A method of reducing the number of microorganisms entering the nose and proliferating in the nasal cavity including application of a solution of an antimicrobial, antiviral and antifungal composition to the anterior vestibular region of the nares. The antimicrobial, antiviral and antifungal solution includes ethyl alcohol as an active ingredient. ... Life Technologies Corporation

05/04/17 / #20170124315

Systems and methods for identifying an individual

The present application relates to systems and methods using biometric data of an individual for identifying the individual and/or verifying the identity of an individual. These systems and methods are useful for, amongst many applications, more secure identification of high-risk individuals attempting to gain access to an entity, transport, information, location, security organization, law enforcement organization, transaction, services, authorized status, and/or funds.. ... Life Technologies Corporation

05/04/17 / #20170121766

Alternative nucleotide flows in sequencing-by-synthesis methods

A method of sequencing a polynucleotide strand can include providing the polynucleotide strand with a primer annealed thereto and a polymerase operably bound to the polynucleotide strand; successively exposing the polynucleotide strand to the flow of four different dntps according to a first predetermined ordering; and successively exposing the polynucleotide strand to the flow of four different dntps according to a second predetermined ordering, wherein the second predetermined ordering is different from the first predetermined ordering.. . ... Life Technologies Corporation

04/27/17 / #20170114391

Polymerization of nucleic acids using proteins having low isoelectric points

This disclosure relates to the use of one or more proteins (e.g., globular proteins) having a low isoelectric point and/or a limited number (e.g., zero) of modifying groups in nucleic acid polymerization and/or amplification reactions such as polymerase chain reaction (pcr).. . ... Life Technologies Corporation

04/27/17 / #20170114321

Methods for eliminating at least a substantial portion of a clonal antigen-specific memory t cell subpopulation

The present invention relates generally to methods for stimulating t cells, and more particularly, to methods to eliminate undesired (e.g., autoreactive, alloreactive, pathogenic) subpopulations of t cells from a mixed population of t cells, thereby restoring the normal immune repertoire of said t cells. The present invention also relates to compositions of cells, including stimulated t cells having restored immune repertoire and uses thereof.. ... Life Technologies Corporation

04/27/17 / #20170114317

Filter-based system and method for efficient capture of lysis of suspended cells

A cell lysis workflow involving a cell suspension that is passed through a filter in a first flow direction from a first side of the filter toward a second side thereof. The filter captures a plurality of cells on the first side. ... Life Technologies Corporation

04/20/17 / #20170108518

Modified nucleic acid binding cyanine dyes for the detection of reactive oxygen species

Disclosed herein are compounds, compositions, methods, and kits for detecting reactive oxygen species (ros) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced nucleic acid binding cyanine dyes, which dyes are probes for detecting ros and measuring oxidative stress in cells either in vitro and/or in vivo. ... Life Technologies Corporation

04/20/17 / #20170108466

Electrophoresis gel with extended shelf life and high performance

An electrophoresis gel with a gel buffer that includes a gel amine buffer, a primary gel ampholyte, and a conjugate gel ampholyte is disclosed herein. The conjugate gel ampholyte may be selected from threonine and serine.. ... Life Technologies Corporation

04/20/17 / #20170108464

System and method for forming microwells

A method of forming a sensor component includes forming a first layer over a sensor pad of a sensor of a sensor array. The first layer includes a first inorganic material. ... Life Technologies Corporation

04/20/17 / #20170107567

Sequence amplification with linear primers

The present disclosure relates to the amplification of target nucleic acid sequences for various sequencing and/or identification techniques. The use of these primers, as described herein, allows for the reduction in the amplification of nonspecific hybridization events (such as primer dimerization) while allowing for the amplification of the target nucleic acid sequences.. ... Life Technologies Corporation

04/20/17 / #20170107539

Ribonucleoprotein transfection agents

Provided herein are compositions and methods useful, inter alia, for the delivery of ribonucleoprotein complexes (e.g., cas9/guide rna complexes) into cells. The compositions and methods provided herein are particularly useful for the delivery of ribonucleoprotein complexes into pluripotent cells and lymphatic cells.. ... Life Technologies Corporation

04/20/17 / #20170105838

Rapid deployment artificial chordae tendinae system

An embodiment includes an apparatus comprising: a hollow needle with a narrowed inner diameter in a distal third of the needle; and a flexible chord coupled to proximal and distal conduits; wherein the distal conduit is in the needle and distal to the narrowed inner diameter and the proximal conduit is in the needle and proximal to the narrowed inner diameter; wherein: (a)(i) long axes of the proximal and distal conduits are substantially parallel to a long axis of the needle when the proximal and distal conduits are in the needle, and (a)(ii) the proximal and distal conduits are configured to rotate when deployed from the needle such that their long axes are not parallel to a long axis of the chord when the chord is fully extended. Other embodiments are described herein.. ... Life Technologies Corporation

04/13/17 / #20170103162

Methods for the determination of a copy number of a genomic sequence in a biological sample

Methods for the determination of a copy number of a target genomic sequence; either a target gene or genomic sequence of interest, in a biological sample are described. Various methods utilize a model drawn from a probability density function (pdf) for the assignment of a copy number of a target genomic sequence in a biological sample. ... Life Technologies Corporation

04/13/17 / #20170101668

Systems and methods for substrate enrichment

A method of separating bead substrates includes applying an emulsion to an emulsion-breaking solution. A dispersed phase of the emulsion includes an unbound polynucleotide, a first set of bead substrates and a second set of bead substrates. ... Life Technologies Corporation

04/06/17 / #20170097317

Chemical detection device having multiple flow channels

The described embodiments may provide a chemical detection circuit that may comprise a plurality of first output circuits at a first side and a plurality of second output circuits at a second side of the chemical detection circuit. The chemical detection circuit may further comprise a plurality of tiles of pixels each placed between respective pairs of first and second output circuits. ... Life Technologies Corporation

04/06/17 / #20170096711

Multi-copy reference assay

A method, comprising amplifying a nucleic acid sequence of interest in a sample comprising genomic dna of a subject; amplifying a reference nucleic acid sequence in the sample; quantifying the amplified sequence of interest relative to the amplified reference sequence; and determining a copy number of the sequence of interest from the relative quantified amplified sequence of interest. The reference sequence may have at least 80% sequence identity to at least one of seq id no:1-38, such as seq id no:1-13. ... Life Technologies Corporation

04/06/17 / #20170096648

Polymerase compositions and kits, and methods of using and making the same

The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. ... Life Technologies Corporation

04/06/17 / #20170095841

Method for treating a semiconductor device

A method of treating a sensor array including a plurality of sensors and an isolation structure, where a sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array and the isolation structure is disposed between the sensor pad and sensor pads of other sensors of the plurality of sensors, comprises exposing the sensor pad and the isolation structure to a non-aqueous organo-silicon solution including an organo-silicon compound and a first non-aqueous carrier; applying an acid solution including an organic acid and a second non-aqueous carrier to the sensor pad; and rinsing the acid solution from the sensor pad and the isolation structure.. . ... Life Technologies Corporation

03/30/17 / #20170088893

Nucleic acid adaptors and uses thereof

Provided herein are compositions, systems, methods, and kits for joining together the ends of one or more polynucleotides using at least one pair of blocking oligonucleotide adaptors. Blocking oligonucleotide adaptors can be used to reduce the formation of adaptor dimers or trimers (or higher-order concatemers) which can improve the yield of desirable polynucleotide-adaptor products in any recombinant nucleic acid workflow. ... Life Technologies Corporation








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