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Life Technologies Corporation patents


Recent patent applications related to Life Technologies Corporation. Life Technologies Corporation is listed as an Agent/Assignee. Note: Life Technologies Corporation may have other listings under different names/spellings. We're not affiliated with Life Technologies Corporation, we're just tracking patents.

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Tool for visualizinig pcr results

In some embodiments, a method or system for visualizing data generated from one or more reaction devices, chips, or reaction sites may be provided. On a scatter plot, data points may be displayed indicative of results from nucleic amplification, wherein the nucleic acid amplification comprises a first target and a second target and the data points are designated as being indicative of amplification of the first target, the second target, both targets, or neither target. ... Life Technologies Corporation

Chemical detection device having multiple flow channels

The described embodiments may provide a chemical detection circuit that may comprise a plurality of first output circuits at a first side and a plurality of second output circuits at a second side of the chemical detection circuit. The chemical detection circuit may further comprise a plurality of tiles of pixels each placed between respective pairs of first and second output circuits. ... Life Technologies Corporation

System and method for acoustic focusing hardware and implementations

The present invention is a method and apparatus for acoustic focusing hardware and implementations.. . ... Life Technologies Corporation

Self-locking door and product dispensing enclosure having a self-locking door

Provided herein is an inventory management system for dispensing products from an enclosure, methods of securing products within the enclosure, use of the enclosure and for maintaining inventory. The inventory management system may include an enclosure having a self-locking door. ... Life Technologies Corporation

Conjugated polymeric particle and method of making same

A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.. . ... Life Technologies Corporation

Device preparation using condensed nucleic acid particles

A method of sequencing a nucleic acid strand includes receiving particles having nucleic acid strands coupled to a polymer matrix, exposing the particles to a solution including a condensing agent, and applying the particles to a surface, the particles depositing on the surface.. . ... Life Technologies Corporation

Novel compounds for use in pcr systems and applications thereof

This disclosure relates to novel compounds for use in various compositions, kits and methods, including, for example, use in polymerase storage buffers and in nucleic acid synthesis or amplification reactions such as a polymerase chain reaction (pcr). Methods for preparing the novel compounds are also described.. ... Life Technologies Corporation

Hydrazinyl and aminooxy compounds and their methods of use

The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.. ... Life Technologies Corporation

Reagent mixer and fluid control devices

An apparatus for preparing a reagent solution includes an enclosure and a container disposed within the enclosure. The container defines an internal cavity having a compressible volume and defines a passage providing fluidic communication between the internal cavity and the exterior of the container. ... Life Technologies Corporation

Chemical coating of microwell for electrochemical detection device

The described embodiments may provide a method of fabricating a chemical detection device. The method may comprise forming a microwell above a cmos device. ... Life Technologies Corporation

Methods for manufacturing well structures for low-noise chemical sensors

In one implementation, a method for manufacturing a chemical detection device is described. The method includes forming a chemical sensor having a sensing surface. ... Life Technologies Corporation

Bioreactor systems with tube supports

A bioreactor system includes a support housing having an interior surface bounding a compartment, the support housing having a sidewall with an opening extending therethrough and communicating with the compartment. A rack is secured to the support housing in alignment with the opening. ... Life Technologies Corporation

Methods, compositions, and kits for detecting allelic variants

In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as snps, or nucleotide (nt) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. ... Life Technologies Corporation

Compositions and methods for immune repertoire sequencing

The present disclosure provides methods, compositions, kits, and systems useful in the determination and evaluation of the immune repertoire. In one aspect, target-specific primer panels provide for the effective amplification of sequences of t cell receptor and/or b cell receptor chains with improved sequencing accuracy and resolution over the repertoire. ... Life Technologies Corporation

07/26/18 / #20180208979

Methods and systems for pcr quantitation

A method for quantifying nucleic acid is provided. The method includes determining a first reference threshold cycle for a first predetermined input quantity for a reference nucleic acid, determining a first target threshold cycle for the first predetermined input quantity for a target nucleic acid, determining a second reference threshold cycle for a second predetermined input quantity for the reference nucleic acid, and determining a second target threshold cycle, by the processor, for the second predetermined input quantity for the target nucleic acid. ... Life Technologies Corporation

07/19/18 / #20180203987

Systems and methods for identifying an individual

The present application relates to systems and methods using biometric data of an individual for identifying the individual and/or verifying the identity of an individual. These systems and methods are useful for, amongst many applications, more secure identification of high-risk individuals attempting to gain access to an entity, transport, information, location, security organization, law enforcement organization, transaction, services, authorized status, and/or funds.. ... Life Technologies Corporation

07/19/18 / #20180202996

Compositions and assays for determining cell viability

Provided are compositions and methods useful in evaluation of cell health and metabolism, cell viability, proliferation, and the effects of compounds on these qualities. The assays provided are rapid, robust, nontoxic and suitable for use with high throughput devices.. ... Life Technologies Corporation

07/19/18 / #20180202966

Ion-sensing charge-accumulation circuits and methods

An ion-sensitive circuit can include a charge accumulation device, to accumulate a plurality of charge packets as a function of an ion concentration of a fluid, and at least one control and readout transistor, to generate an output signal as a function of the accumulated plurality of charge packets, the output signal representing the ion concentration of the solution. The charge accumulation device can include a first charge control electrode above a first electrode semiconductor region, an electrically floating gate structure above a gate semiconductor region and below an ion-sensitive passivation surface, a second charge control electrode above a second electrode semiconductor region, and a drain diffusion region. ... Life Technologies Corporation

07/19/18 / #20180202936

Modified hydrocyanine dyes for the detection of reactive oxygen species

The present invention is directed to compounds, compositions, methods, and kits for detecting reactive oxygen species (ros) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced dyes, including cy-based hydrocyanine dyes and cy-based deuterocyanine dyes, which dyes are probes for detecting ros and measuring oxidative stress in cells either in vitro and/or in vivo. ... Life Technologies Corporation

07/19/18 / #20180201907

Methods for increasing the infectivity of viruses

Methods of using viruses labeled with alkyne-modified biomolecules, such as fatty acids, carbohydrates and lipids, to treat a plant, an insect or an animal infected with a virus or to increase the infectivity of a virus, such as the human immunodeficiency virus, are provided. Also provided are methods of labeling a virus, such as human immunodeficiency virus, with an alkyne-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. ... Life Technologies Corporation

07/19/18 / #20180200374

Amine-containing transfection reagents and methods for making and using same

There are provided for herein novel amine-containing transfection compounds and methods for making and using same. The compounds are generally obtained by reacting a primary amine with an unsaturated compound. ... Life Technologies Corporation

07/12/18 / #20180196915

Methods and systems for nucleic acid sequence analysis

Disclosed are new and improved methods and systems for nucleic acid sequence analysis that can analyze data indicative of natural by-products of nucleotide incorporation events without the need for exogenous labels or dyes to identify nucleic acid sequences of interest. In particular, the methods and systems of the present teachings can process such data and various forms thereof to align fragments of the nucleic acid(s) of interest, particularly those analyzed using an addition sequencing technique, for example, as occurs with the use of nucleotide flows.. ... Life Technologies Corporation

07/12/18 / #20180196033

Cell tracking reagents and their methods of use

Described herein are compounds, methods, and kits for short- and long-term tracking of cell proliferation, differentiation, and/or function. The compounds disclosed herein are coumarin-based cell-tracking reagents that fluoresce in the blue portion of the uv/vis spectrum and provide bright fluorescence intensity, uniform cell staining, good retention within cells and low toxicity toward cells.. ... Life Technologies Corporation

07/12/18 / #20180195057

Purification of nucleic acid from environmental or biological samples

Compositions and methods for nucleic acid isolation from an environmental or biological sample comprising nucleic acid-analysis interferents, particularly from microbiome-containing samples, are provided.. . ... Life Technologies Corporation

07/12/18 / #20180195034

Methods and apparatus for gas stream mass transfer with a liquid

A system for performing a gas-liquid mass transfer includes a container bounding a compartment and having a top wall, a bottom wall, and an encircling sidewall extending therebetween. A tube has a first end and an opposing second end, the first end of the tube being disposed within the compartment of the container. ... Life Technologies Corporation

07/05/18 / #20180187809

Compression collars for coupling a tube to a tube fitting and methods of use

A method for coupling a tube to a tube fitting includes radially outwardly expanding a tubular compression collar from a constricted state to an expanded state, the compression collar having a throughway extending there through and being made of a resiliently flexible material. An end of the tube is inserted within the throughway of the expanded compression collar, the tube bounding a passageway. ... Life Technologies Corporation

06/28/18 / #20180181707

Methods, systems and computer readable media to correct base calls in repeat regions of nucleic acid sequence reads

Methods, systems and non-transitory machine-readable storage medium are provided to mitigate insertion errors and deletion errors in str sequences and improve accuracy in determination of the number of repeats. A method includes determining one or more optimum clusters for a set of flow space signal measurements, wherein at least one of the optimum clusters is associated with a homopolymer length, modifying a base call at the position in the repeat region sequence to the homopolymer length associated with the optimum cluster to produce a corrected repeat region sequence, thereby correcting an insertion error or a deletion error. ... Life Technologies Corporation

06/28/18 / #20180180572

Chemical device with thin conductive element

In one implementation, a chemical device is described. The sensor includes a chemically-sensitive field effect transistor including a floating gate structure having a plurality of floating gate conductors electrically coupled to one another. ... Life Technologies Corporation

06/28/18 / #20180179548

Molecular biology tools for algal engineering

The present invention provides compositions and methods for the genetic manipulation of algal cells. The compositions and methods allow enhanced transfer of genetic material into algal cells and the cloning and selection of genetically modified cells. ... Life Technologies Corporation

06/28/18 / #20180179520

Scaffolded nucleic acid polymer particles and methods of making and using

The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. ... Life Technologies Corporation

06/21/18 / #20180171400

Small rna capture, detection and quantification

Methods, compositions and kits for capturing, detecting and quantifying mature small rnas are provided herein. Embodiments of the methods comprise ligating 5′ and 3′ ligation adaptors to the 5′ and 3′ ends of the mature small rnas, respectively, in the presence of 5′ and 3′ semi-degenerate ligation splints to generate a ligation product. ... Life Technologies Corporation

06/14/18 / #20180165410

Methods for detecting mutation load from a tumor sample

A targeted panel with low sample input requirements from a tumor only sample may be processed to estimate mutation load in a tumor sample. The method, including detecting variants in nucleic acid sequence reads corresponding to targeted locations in the tumor sample genome; annotating detected variants with an annotation information from a population database; filtering the detected variants, wherein the filtering rule set retains the somatic variants and removes germ-line variants; counting the identified somatic variants to give a number of somatic variants; determining a number of bases in covered regions of the targeted locations in the tumor sample genome; and calculating a number of somatic variants per megabase, provides an estimate of the mutation load per megabase in the tumor sample genome.. ... Life Technologies Corporation

06/14/18 / #20180163255

Nucleic acid preparation method

A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support whilst maintaining it on the support. ... Life Technologies Corporation

06/14/18 / #20180163231

Membrane-penetrating peptides to enhance transfection and compositions and methods for using same

The present invention is directed to non-naturally occurring peptides containing a membrane-penetrating amino acid sequence and further at least one polycationic moiety or peptide sequence. The peptides are suitable for use in delivery a cargo to the interior of a cell. ... Life Technologies Corporation

06/07/18 / #20180158907

Nanocrystals with high extinction coefficients and methods of making and using such nanocrystals

A population of bright and stable nanocrystals is provided. The nanocrystals include a semiconductor core and a thick semiconductor shell and can exhibit high extinction coefficients, high quantum yields, and limited or no detectable blinking.. ... Life Technologies Corporation

06/07/18 / #20180157788

Systems and methods for genotyping by angle configuration search

Methods and systems for the analysis of genotyping data are presented. According to various embodiments of methods and systems, an angle configuration search may be performed. ... Life Technologies Corporation

06/07/18 / #20180157023

Systems and methods for stabilizing a sample stage in a structured illumination imaging system

An apparatus for generating composite confocal images, comprising: an imaging apparatus configured to generate illumination; a sample stage configured to hold a sample to be illuminated by the illumination, wherein the illumination causes the sample to generate emissions that can be detected and used to image the sample, the sample stage configured to move the sample to a plurality of positions during the imaging process; and a vibration mechanism coupled with the sample stage, the motor configured to vibrate the stage after the stage moves the sample for a vibration period.. . ... Life Technologies Corporation

06/07/18 / #20180157022

Systems and methods for calibrating a structured illumination imaging system and for capturing a structured illumination image

A method for calibrating an imaging system, comprising: illuminating a sample through a pinhole mask using an excitation light; capturing an image of the sample using a sensor; converting the image into data; in a processing module: filtering the data using known spacing of pinholes in the pinhole mask to obtain data that corresponds to the spacing, using a threshold to identify regions of the remaining data that are bright enough to be associated with a pinhole, calculating the centroids of the regions, and fitting a known pattern for the pinhole mask to the regions in order to identify the best fit for the data; and storing, in a storage medium, the best fit data for use in a subsequent confocal capture routine.. . ... Life Technologies Corporation

06/07/18 / #20180157021

Systems and methods for generating a structured illumination image

A method for generating a composite image obtained in a fluorescence imaging system, comprising: determining the physical locations of a pinhole mask relative to a sample required to construct a full composite confocal image; generating in a control module a randomized order for the determined physical locations; moving the photomask or the sample to the determined physical locations in the randomized order under control of the control module and using a translation stage; illuminating the sample through the photomask using an excitation light; capturing a plurality of images at each of the physical locations using a sensor in order to generate a set of data points; using the randomized order to generate a composite image based on the set of data points and measuring the brightness of at least some of the data points; and adjusting the brightness of some of the set of data points.. . ... Life Technologies Corporation

06/07/18 / #20180156703

Method of preparing quality control material for ffpe

This specification relates to formalin-fixed embedded quality control material for use for validation, verification, and to run controls for molecular assays. The quality control material can be used for a variety of tissues and for a variety of molecular assays. ... Life Technologies Corporation

06/07/18 / #20180155795

Classification and actionability indices for cancer

The disclosure provides compositions, kits, and methods for detecting a plurality of genes and associated variants in a sample from a subject with cancer. The compositions, kits, and methods include a set of oligonucleotides, typically primers and/or probes that can hybridize to identify a gene variant. ... Life Technologies Corporation

06/07/18 / #20180154289

Microcarrier filter bag assemblies and methods of use

A filter bag assembly includes a flexible bag bounding a compartment that is configured to hold a fluid. An inlet port and an outlet port are each secured to the flexible bag so as to communicate with the compartment. ... Life Technologies Corporation

05/31/18 / #20180149847

Systems and methods for an interchangeable illumination filter set for use in a structured illumination imaging system

An apparatus for generating structured illumination images, comprising a housing; imaging optics mounted within the housing, the imaging optics configured to focus illumination from an illumination source such that an in-focus pattern of light corresponding to a pattern on a photomask is projected onto a sample; a reflector mounted within the housing and configured to reflect the illumination to a sample; a filter apparatus, comprising one or more filters mounted within the housing, the filter apparatus configured to allow only emissions from the illuminated sample to pass through to the sensor; and mounting features on the housing, the mounting features configured to allow the apparatus to be installed within an imaging system.. . ... Life Technologies Corporation

05/24/18 / #20180144094

Determining the limit of detection of rare targets using digital pcr

A method for determining false positives calls in a biological data plot is provided. The method includes identifying a first data cluster as non-amplification data points within the biological data plot and identifying a second data cluster as wild-type positives within the biological data plot. ... Life Technologies Corporation

05/24/18 / #20180142203

Pellets used in cell culture and methods of making thereof

The present invention is directed generally to dry cell culture media or feeds in pellet formats which can be reconstituted into liquid media for culturing cells in vitro. Each pellet composition may comprise the same or a different composition; for example, different vitamins, amino acids, buffers, trace salts, ph, iron chelators, etc. ... Life Technologies Corporation

05/17/18 / #20180135077

Serum-free suspension system for lentiviral production

A lentiviral vector production system comprises (a) a lentiviral culture supplement to control cell growth, (b) a transfection reagent comprising dhdms, dope, and cholesterol to increase transfection efficiency, (c) a lentiviral production enhancer comprising sodium propionate, sodium butyrate, and caffeine to boost lentiviral production, wherein the lentiviral vector production system is serum-free. A method of lentiviral vector production comprises using the lentiviral production system. ... Life Technologies Corporation

05/10/18 / #20180128773

Chemical sensor with consistent sensor surface areas

. . A chemical sensor is described. The chemical sensor includes a chemically-sensitive field effect transistor including a floating gate conductor having an upper surface. ... Life Technologies Corporation

05/10/18 / #20180127706

Filter-based method for efficient capture of lysis of suspended cells

A cell lysis workflow involving a cell suspension that is passed through a filter in a first flow direction from a first side of the filter toward a second side thereof. The filter captures a plurality of cells on the first side. ... Life Technologies Corporation

05/03/18 / #20180120575

Devices, systems and methods for illuminating and imaging objects

An illumination system includes a surface configured to have an imaging target placed thereon, a light source, a beam splitter and at least a first mirror. The beam splitter is configured to split the beam of light from the light source and the first mirror is configured to reflect a first beam from the beam splitter onto the surface with the imaging target. ... Life Technologies Corporation

05/03/18 / #20180119217

Modified nucleotides and uses thereof

In some embodiments, the disclosure relates generally to methods, as well as related, systems, compositions, kits and apparatuses, for nucleic acid analysis that involve the use of modified nucleotides, including terminator nucleotides and/or tagged nucleotides, in a template-dependent nucleotide incorporation reaction. In some embodiments, the nucleic acid analysis can be conducted at a single reaction site, or at a plurality of reaction sites in an array of reaction sites. ... Life Technologies Corporation

05/03/18 / #20180117546

Liquid mixing system with vertically adjustable mixing element and method of use

A liquid mixing system includes a support housing at least partially bounding a compartment. A mount is secured to the support housing. ... Life Technologies Corporation

04/19/18 / #20180106756

Chemical sensor with air via

In one embodiment, a chemical sensor is described. The chemical sensor includes a chemically-sensitive field effect transistor including a floating gate conductor having an upper surface, a first opening extending through a first material and through a portion of a second material located on the first material and a second opening extending from the bottom of the first opening to the top of a liner layer located on the upper surface of the floating gate conductor.. ... Life Technologies Corporation

04/19/18 / #20180105805

Linking methods, compositions, systems, kits and apparatuses

In some embodiments, the disclosure relates generally to methods as well as related compositions, systems, kits and apparatus comprising linking proteins to target compounds and/or to locations of interest using tethers. For example, the tether can be used to link the protein to a target compound, for example, to link an enzyme to a substrate. ... Life Technologies Corporation

04/19/18 / #20180104790

Systems and methods for run-time sequencing run quality monitoring

Systems and methods of providing run-time quality control and monitoring of a single or multiple sequencing runs are provided herein. In some embodiments, the run-time system includes or is in communication with a processor capable of determining various types of run-time information relating to the quality, progress, etc. ... Life Technologies Corporation

04/12/18 / #20180100198

Gene fusion

The disclosure provides gene fusion variants and novel associations with disease states, as well as kits, probes, and methods of using the same.. . ... Life Technologies Corporation

04/12/18 / #20180100194

Protein detection using fet

Accordingly, in some embodiments methods for detecting an analyte or analytes in one or more sample(s) are provided. The methods encompass providing a solid support with an addressable marker and an associated ligand, contacting the solid substrate to a sample, thereby forming a contacted solid support, associating the contacted solid support with a fet array and detecting the electrical properties of the fet array and thereby detecting an analyte or analytes in one or more samples. ... Life Technologies Corporation

04/12/18 / #20180100191

Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis

Nucleotide triphosphate probes containing a molecular and/or atomic tag on a γ and/or β phosphate group and/or a base moiety having a detectable property are disclosed, and kits and method for using the tagged nucleotides in sequencing reactions and various assay. Also, phosphate and polyphosphate molecular fidelity altering agents are disclosed.. ... Life Technologies Corporation

04/05/18 / #20180094297

Gene expression profiling from ffpe samples

Methods and compositions relating to the generation and use of gene expression data from tissue samples that have been fixed and embedded are provided. The data can electronically stored and implemented as well as used to augment diagnosis and treatment of diseases.. ... Life Technologies Corporation

04/05/18 / #20180094250

Methods and compositions for pcr

A modified thermostable pol b dna polymerase, produced by a reaction, under essentially aqueous conditions, of a thermostable pol b dna polymerase and a modifier reagent of formula i wherein the reaction results in a thermally reversible inactivation of the thermostable pol b dna polymerase activity and the 3′-5′ exonuclease activity, which polymerase is suitable for hot-start pcr. Also disclosed are the method for the modification, a polynucleic acid amplification method and pcr reaction mixture and kit comprising the modified thermostable pol b dna polymerase.. ... Life Technologies Corporation

04/05/18 / #20180092638

System and method for rapid attachment of cardiovascular prosthesis

An embodiment includes a system comprising: first and second conduits respectively including first and second barbed rods; first and second shafts; wherein: (a)(i) in a first configuration the first shaft slides distally within the first conduit and couples to a proximal end of the first barbed rod, and (a)(ii) in a second configuration the first shaft slides distally within the first conduit and pushes the first barbed rod out of the first conduit; wherein: (a)(i) in an additional first configuration the second shaft slides distally within the second conduit and couples to a proximal end of the second barbed rod, and (a)(ii) in an additional second configuration the second shaft slides distally within the second conduit and pushes the second barbed rod out of the second conduit. Other embodiments are described.. ... Life Technologies Corporation

03/29/18 / #20180089366

Systems and methods for mapping sequence reads

Systems, methods, and computer program products for aligning a fragment sequence to a target sequencing. The alignment is allowed at most one gap, such as an insertion or a deletion. ... Life Technologies Corporation

03/29/18 / #20180087056

Oligonucleotide compositions with enhanced efficiency

The oligonucleotide compositions of the present invention make use of combinations of oligonucleotides. In one aspect, the invention features an oligonucleotide composition including at least 2 different oligonucleotides targeted to a target gene. ... Life Technologies Corporation

03/29/18 / #20180087048

Alkylene glycols and polymers and copolymers thereof for direct isolation of nucleic acid from embedded samples

Methods of directly isolating nucleic acid from an embedded biological sample are provided. An emulsified digest is generated in the presence of a thermostable protease, and an additive selected from an alkylene glycol, a poly(alkylene glycol), or a block copolymer having an average mn of 76 to 2900, or a salt or derivative or combination thereof. ... Life Technologies Corporation

03/22/18 / #20180078915

Bioreactor with impeller assembly

A bioreactor includes a flexible container and an impeller assembly having a hollow tube. The impeller assembly is coupled to the flexible container. ... Life Technologies Corporation

03/22/18 / #20180078662

Methods and compositions for enzyme-mediated site-specific radiolabeling of glycoproteins

Provided herein are methods, compositions and kits for use in the site-specific labeling of glycoproteins comprising a combination of enzyme-mediated incorporation of modified sugars comprising a chemical handle and cycloaddition chemistry with a labeling molecule comprising a reactive group, a metal ion chelator, and/or a fluorophore.. . ... Life Technologies Corporation

03/15/18 / #20180073055

Nucleic acid extraction and amplification controls and methods of use thereof

Nucleic acid reagents and corresponding methods of using the same for monitoring and evaluating nucleic acid extraction and amplification reactions.. . ... Life Technologies Corporation

03/15/18 / #20180073009

Compositions and methods for preparing short rna molecules and other nucleic acids

The invention provides methods of preparing nucleic acids, such as rna molecules, of a defined size or range of sizes. The invention provides compositions, methods and kits for use in the production and preparation of small rna molecules (including without limitation micro-rna, sirna, d-sirna and e-sirna) and other nucleic acids of various sizes.. ... Life Technologies Corporation

03/15/18 / #20180071700

Fluid mixing system with flexible drive line and foldable impeller

A fluid mixing system includes a container, such as a flexible bag, bounding a compartment. A flexible drive line is disposed within the compartment, the drive line having a first end rotatably connected to a first end of the container and an opposing second end rotatably connected to a second end of the container. ... Life Technologies Corporation

03/08/18 / #20180068061

Systems and methods for detecting homopolymer insertions/deletions

Systems and method for determining variants can receive mapped reads and determine a distribution of matched-filter residuals distribution from a plurality of reads at a homopolymer region. The distribution of matched-filter residuals can be fit to uni-modal and bi-modal models. ... Life Technologies Corporation

03/08/18 / #20180067044

Apparatuses, systems, methods, and computer readable media for acoustic flow cytometry

A flow cytometer includes a capillary having a sample channel; at least one vibration producing transducer coupled to the capillary, the at least one vibration producing transducer being configured to produce an acoustic signal inducing acoustic radiation pressure within the sample channel to acoustically concentrate particles flowing within a fluid sample stream in the sample channel; and an interrogation source having a violet laser and a blue laser, the violet and blue lasers being configured to interact with at least some of the acoustically concentrated particles to produce an output signal.. . ... Life Technologies Corporation

03/08/18 / #20180067037

Systems, methods, and apparatuses for optical systems in flow cytometers

The present set of embodiments relate to a system, method, and apparatus for an optical configuration in a flow cytometer that allows for independent adjustment of focusing for each light source. Such systems, methods, and apparatuses require a final focusing element to be moved near the beginning of the optical train and for each optical element coming after the final focusing element to be configured to accommodate converging light beams while minimizing the introduction of aberrations into those beams.. ... Life Technologies Corporation

03/01/18 / #20180060481

Identification, monitoring and treatment of infectious disease and characterization of inflammatory conditions related to infectious disease using gene expression profiles

A method is provided in various embodiments for determining a profile data set for a subject with infectious disease or inflammatory conditions related to infectious disease based on a sample from the subject, wherein the sample provides a source of rnas. The method includes using amplification for measuring the amount of rna corresponding to at least 2 constituents from table 1. ... Life Technologies Corporation

03/01/18 / #20180057814

Compositions and methods for inhibition of nucleic acids function

The invention relates generally to compositions and methods for inhibiting the function of target nucleic acids by sequence specific binding. The compositions and methods can be used for inhibition of micro rnas and other relatively short non-coding rnas.. ... Life Technologies Corporation

03/01/18 / #20180056296

Apparatuses, systems and methods for providing thermocycler thermal uniformity

A thermal block assembly including a sample block and two or more thermoelectric devices, is disclosed. The sample block has a top surface configured to receive a plurality of reaction vessels and an opposing bottom surface. ... Life Technologies Corporation

02/15/18 / #20180044371

Methods for isolation, identification, and quantification of mirnas

Method and compositions and kits for isolation, identification, and quantification of mirnas and other small rnas, including but not limited to, sirnas, mrnas, and snrnas are disclosed. Methods of diagnosing a disease or its progression are also disclosed.. ... Life Technologies Corporation

02/08/18 / #20180037931

Chemically-enhanced primer compositions, methods and kits

A chemically-enhanced primer is provided comprising a negatively charged moiety (ncm), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. ... Life Technologies Corporation

02/01/18 / #20180031545

Charged reactive oligomers

Methods, systems, compositions and kits are described for detecting cleaved glycans from a glycoconjugate. After labeling the glycan with a nucleic acid charged oligomer described herein, the labeled glycans can be separated under the influence of an electric field or based on their detectable tag and identified.. ... Life Technologies Corporation

02/01/18 / #20180031522

Sharply resolving labeled protein molecular weight standards

Pre-labeled protein standards useful in electrophoresis that have sharp, consistent separation characteristics that are substantially the same as those of their unlabeled counterparts are provided. The invention provides pre-labeled protein standard sets that include a plurality of labeled proteins that are labeled on a first amino acid, in which side reactions of the label with amino acids not targeted for labeling are reduced.. ... Life Technologies Corporation

02/01/18 / #20180030534

Bi-directional sequencing compositions and methods

In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, in some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.. . ... Life Technologies Corporation

01/25/18 / #20180024094

Electrophoresis gel cassette and comb

Provided herein is an apparatus for gel electrophoresis comprising a cassette and a comb having at least one wedge-shaped tooth.. . ... Life Technologies Corporation

01/25/18 / #20180023075

Compositions and methods for enhancing homologous recombination

The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.. ... Life Technologies Corporation

01/18/18 / #20180018422

Systems and methods for nucleic acid-based identification

Systems and methods for calculating a predictive index of identity of a nucleic acid sample using polymorphic genetic marker data are provided. In one embodiment, a predictive index of identity of the nucleic acid sample is calculated using a value from a second set of data from a polymorphic genetic marker that is not linked to a polymorphic genetic marker used to produce a first set of data. ... Life Technologies Corporation

01/18/18 / #20180017523

Electro-blotting devices, systems, and kits and methods for their use

The invention provides a dry electroblotting system for dry blotting gels, in which the system includes an electroblotting transfer stack that comprises an analysis gel and a blotting membrane, an anode, a body of anodic gel matrix juxtaposed with the anode between the anode and the transfer stack, a cathode, and a body of cathodic gel matrix juxtaposed with the cathode between the cathode and the transfer stack, in which the anodic gel matrix and the cathodic gel matrix each comprise an ion source for electrophoretic transfer. The dry electroblotting system does not use any liquid buffers that are added to the system just before electroblotting (such as when the transfer stack is being assembled). ... Life Technologies Corporation

01/18/18 / #20180016572

Compositions and methods for detecting nucleic acid regions

Provided are methods, compositions, reagents, kits that are useful for detecting a specific nucleic acid region in genome with high efficiency and high sensitivity.. . ... Life Technologies Corporation

01/11/18 / #20180010177

Enhanced ligation reactions

In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. ... Life Technologies Corporation

01/11/18 / #20180010105

Novel reverse transcriptases for use in high temperature nucleic acid synthesis

The invention provides novel reverse transcriptases (rts) with desirable properties such as increased thermostability, increased thermoreactivity and/or increased resistance to inhibitors. In certain embodiments, the invention provides methods of producing, amplifying and/or sequencing nucleic acid molecules (particularly cdna molecules) using kits, compositions and/or reactions mixtures containing such novel reverse transcriptase enzymes.. ... Life Technologies Corporation

01/11/18 / #20180008410

Heart valve sewing cuff

An embodiment of the invention includes a sewing cuff for aortic heart valves that better approximates native anatomy by better mating with the crown-like anatomical annulus. Limiting distortion of the crown-like annulus provides better blood flow and overall valve function and provides a physician greater ease of implantation since native anatomy is not flattened. ... Life Technologies Corporation

01/04/18 / #20180003672

Method for treating a semiconductor device

A sensor array includes a plurality of sensors. A sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array. ... Life Technologies Corporation

12/28/17 / #20170369857

Nucleotide transient binding for sequencing methods

Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (snp) analyses. ... Life Technologies Corporation

12/28/17 / #20170369431

Hydrazinyl and aminooxy compounds and their methods of use

The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.. ... Life Technologies Corporation

12/21/17 / #20170362640

Novel compositions, methods and kits for microorganism detection

Methods, compositions and kits for detecting microorganisms and/or profiling microbiota such as for example through use of nucleic acid amplification and detection.. . ... Life Technologies Corporation

12/21/17 / #20170362624

Labeling and detection of post translationally modified proteins

Provided in certain embodiments are new methods for forming azido modified biomolecule conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling a biomolecules with an azide group.. ... Life Technologies Corporation

12/14/17 / #20170354901

Acoustically settled liquid-liquid sample purification system

A sample purification system includes a container assembly bounding a sample purification compartment and having an upper end and an opposing lower end, the sample purification compartment comprising mixing zones and settling zones. A plurality of shielding elements are positioned within the sample purification compartment so as to at least partially separate adjacent mixing zones and settling zones or separate adjacent mixing zones, the mixing zones being in fluid communication with the settling zones. ... Life Technologies Corporation

12/07/17 / #20170351807

Methods and systems for designing gene panels

A system and method of selecting genes for a gene panel, includes retrieving gene-disease associations of genes associated with diseases at a given level in the disease hierarchy from a disease association database. The disease association database stores disease information, gene information, phenotype information, associations between diseases in the disease hierarchy, gene-disease associations and strength parameters related to the gene-disease associations. ... Life Technologies Corporation

11/30/17 / #20170342371

Method of culturing a cell with a supplement

Cell culture media, concentrated media and feeds, methods of manufacturing cell culture media and feeds, and methods of culturing cells are provided. One or more small peptides, including dipeptides are added to the cell culture media to provide improved stability and improved conditions for culturing cells.. ... Life Technologies Corporation

11/23/17 / #20170337507

Inventory management system and method of use

Provided is a computer-implemented method for product management the method can include obtaining user identification information from a user, wherein the identification code is associated with a financial account, of the user; validating, by a processor, that the identification information is authentic based on registration information that was previously received; providing first access signal to a locking mechanism of a locking enclosure to unlock the locking mechanism based on the validating, wherein the locking enclosure securely stores a purchasable product; obtaining product identification information from a scan of a bar code associated with the product providing the product information that was obtained to be rendered on a display; receiving an indication from the user that the product information is correct; and providing a second access signal to the locking mechanism to lock the locking mechanism based on the indication that was received.. . ... Life Technologies Corporation

11/23/17 / #20170335387

Systems and methods for identifying sequence variation

Systems and method for determining variants can receive mapped reads, align flow space information to a flow space representation of a corresponding portion of the reference. Reads spanning a position with a potential variant can be evaluated in a context specific manner. ... Life Technologies Corporation

11/23/17 / #20170335377

Se33 mutations impacting genotype concordance

Disclosed are primer set compositions, methods and kits for human identification using the highly complex sequence locus, se33 (actbp2) in single and multiplex pcr reactions. Additionally, disclosed are three newly discovered single nucleotide polymorphisms (snps) within the se33 locus that can cause discordance seen as mobility shift or allelic dropout. ... Life Technologies Corporation

11/23/17 / #20170333457

Anti-viral azide containing compounds

Methods of using azide-modified biomolecules, such as fatty acids, carbohydrates and lipids, to treat a plant, an insect or an animal infected with a virus or to inhibit infectivity of a virus, such as the human immunodeficiency virus, are provided. Also provided are methods of labeling a virus, such as human immunodeficiency virus, with an azide-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. ... Life Technologies Corporation

11/16/17 / #20170330327

Systems, methods, and apparatuses for image capture and display

Provided are systems and methods that allow a user to capture images at low- and high-level magnification and then overlay the high-level magnification images on the low-level magnification image to ease review of the images. The high-level magnification images may be overlaid on the low-level magnification image based at least in part on the portion of the low-level magnification image from which the high-level image was originated.. ... Life Technologies Corporation

11/16/17 / #20170328862

Expanding cam lock for sealing slab gels in an electrophoresis apparatus

An expanding cam lock for use with an electrophoresis system is disclosed herein. The cam lock allows the simultaneous use of multiple slab gel cassettes in first and second buffer core assemblies in an electrophoresis system while maintaining the necessary compressive force to create a liquid-tight seal between the anode and cathode buffer solutions. ... Life Technologies Corporation

11/16/17 / #20170327902

Penta e polymorphisms for human identification

Disclosed herein are methods and compositions for human identification using polymorphisms in the penta e short tandem repeat locus. These newly disclosed polymorphisms are significant in preventing allelic drop out.. ... Life Technologies Corporation

11/09/17 / #20170321271

Combinatorial barcode sequences, and related systems and methods

A kit for use with a nucleic acid sequencing instrument can include a plurality of combinatorial barcodes sequences meeting the following criteria: each of the combinatorial barcode sequences comprise a plurality of iterations of a sequence motif, where the sequence motif comprises a first nucleotide base from a first group of nucleotide bases followed by a second nucleotide base from a second group of nucleotide bases, the first group and the second group differing from each other; and the plurality of combinatorial barcode sequences is at least 1,000,000 different barcode sequences.. . ... Life Technologies Corporation

11/09/17 / #20170321269

Method of determining the nucleotide sequence of oligonucleotides and dna molecules

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of dna polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a dna fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. ... Life Technologies Corporation

11/09/17 / #20170321245

Flash and glow 1,2-dioxetanes

Compounds having chemiluminescent flash and glow properties. Also disclosed are methods using the compounds to generate light, detect and/or quantify enzymes, antigens, and/or nucleic acids. ... Life Technologies Corporation

11/09/17 / #20170321219

Oligonucleotide compositions with enhanced efficiency

The oligonucleotide compositions of the present invention make use of combinations of oligonucleotides. In one aspect, the invention features an oligonucleotide composition including at least 2 different oligonucleotides targeted to a target gene. ... Life Technologies Corporation

10/26/17 / #20170307529

Optical systems and methods for biological analysis

An instrument for processing and/or measuring a biological process contains an excitation source, a sample holder, an optical sensor, an excitation optical system, and an emission optical system. The sample holder is configured to receive a plurality of biological samples. ... Life Technologies Corporation

10/26/17 / #20170307447

Probe port and realted container system

A container system includes a flexible bag, a probe port, and a probe. The probe port includes an elongated tubular member having an interior surface bounding a first passageway extending between a first end and an opposing second end, the tubular member being made of a polymeric or elastomeric material and being flexible. ... Life Technologies Corporation

10/26/17 / #20170306306

Compositions and methods for enhancing homologous recombination

The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.. ... Life Technologies Corporation

10/26/17 / #20170306072

Particles containing multi-block polymers

Polymer particles having a multi-block vinylic polymer attached to their surface are disclosed. The particles can be used in a variety of purification and detection methods.. ... Life Technologies Corporation

10/26/17 / #20170305954

Synthesis of 2', 3'-dideoxynucleosides for automated dna synthesis and pyrophosphorolysis activated polymerization

Methods for preparation of 2′,3′-dideoxynucleotides support structures, such as 2′,3′-dideoxyguanosine, 2′,3′-dideoxyadenosine, and 3′-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated dna synthesis and pyrophosphorolysis activated polymerization.. ... Life Technologies Corporation

10/12/17 / #20170293713

Models for analyzing data from sequencing-by-synthesis operations

A method of modeling a background signal when sequencing a polynucleotide strand using sequencing-by-synthesis includes: flowing a series of nucleotide flows onto a reactor array having multiple reaction confinement regions, one or more copies of the polynucleotide strand being located in a loaded reaction confinement region of the reactor array, the loaded reaction confinement region being located in a vicinity of one or more neighboring reaction confinement regions that may or may not be loaded; receiving output signals from the reactor array; and modeling a background signal for the loaded reaction confinement region using the received output signals and a model adapted to account at least for an exchange of ions between the one or more neighboring reaction confinement regions and a headspace adjacent the loaded reaction confinement region and the one or more neighboring reaction confinement regions.. . ... Life Technologies Corporation

10/12/17 / #20170292156

Modified proteins and methods of making and using same

Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pka values are described. ... Life Technologies Corporation

10/12/17 / #20170292144

Compositions, methods, and kits for synthesis and detection of nucleic acids

Compositions, methods, and kits for synthesizing, detecting, and/or quantifying nucleic acids are provided herein. Embodiments comprise a nucleic acid amplification composition comprising a thermostable dna polymerase and agents which improve nucleic acid synthesis, amplification, detection, and/or quantification of nucleic acid targets in a crude extract or crude lysate sample.. ... Life Technologies Corporation

10/05/17 / #20170284907

Automated microdissection instrument

Systems and methods for automated laser microdissection are disclosed including automatic slide detection, position detection of cutting and capture lasers, focus optimization for cutting and capture lasers, energy and duration optimization for cutting and capture lasers, inspection and second phase capture and/or ablation in a quality control station and tracking information for linking substrate carrier or output microdissected regions with input sample or slide.. . ... Life Technologies Corporation

10/05/17 / #20170283868

Methods and apparatus for single molecule sequencing using energy transfer detection

Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. ... Life Technologies Corporation

09/28/17 / #20170275688

Methods, compositions and systems for sample deposition

Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. ... Life Technologies Corporation

09/28/17 / #20170275674

Next-generation sequencing quality control monitoring tool

Systems and methods are used to provide an online tool for clinical laboratories to monitor next-generation sequencing (ngs) workflow using quality control (qc) material that can be used for multiple assays. The tool utilizes a highly multiplexed qc with ngs assays that detect somatic mutations. ... Life Technologies Corporation

09/21/17 / #20170269109

Method and apparatus for automated sample manipulation

The present teachings provide apparatuses and methods for automated handling of samples, e.g., biological or chemical samples. The apparatuses and the methods of the present teachings allow automated performance of various sample manipulation steps without manual intervention. ... Life Technologies Corporation

09/14/17 / #20170260512

Polymerase compositions, methods of making and using same

The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. ... Life Technologies Corporation

09/07/17 / #20170254775

Isotopically-labeled proteome standards

The invention provides methods for quantifying biomolecules, such as polypeptides in mass spectrometric analysis. The methods include use of a biomolecule standard having at least one atomic isotope different than that of the naturally occurring isotopes in the biomolecule of interest. ... Life Technologies Corporation

09/07/17 / #20170253922

Human identification using a panel of snps

The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex pcr of one or more nucleic acids which belong to a panel of single nucleotide polymorphisms (snps) useful to identify a human. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences in the panel. ... Life Technologies Corporation

09/07/17 / #20170253921

Methods, kits & compositions for determining gene copy numbers

The present specification relates to methods, compositions, and kits for determining the presence of the number of copies of a gene. The present disclosure provides methods and compositions to determine zygosity in transgenic plants, number of transgenes in a plant or animal, karyotyping, determine cnv in human and animal samples, diagnose and detect diseases and conditions that are characterized by having variations in the number of copies of one or more genes.. ... Life Technologies Corporation

08/31/17 / #20170248606

Apparatuses, methods, computer program products, and kits for hi-throughput glycan analysis

An apparatus for glycan analysis is disclosed. The apparatus includes a plurality of loading wells adapted to receive a plurality of samples; a plurality of capillaries arranged in correspondence with the loading wells, each of the capillaries including a first portion including a stacking gel and a second portion including a resolving gel; and a plurality of eluting wells arranged in correspondence with the capillaries and adapted to receive a portion of the samples having traversed the capillaries.. ... Life Technologies Corporation

08/31/17 / #20170247756

Genetic sequence verification compositions, methods and kits

Methods, compositions and kits are described for resequencing, confirming or verifying next generation sequencing (ngs) results with sanger sequencing. These methods are particularly useful for samples having very limited quantities such as formalin-fixed, paraffin-embedded (ffpe), laser capture microdissection (lcm), fine needle biopsies or aspirates.. ... Life Technologies Corporation

08/31/17 / #20170247504

Dibenzosilole monomers and polymers and methods for their preparation and use

Water-soluble, conjugated polymers containing one or more dibenzosilole monomer residues, as well as compositions, kits, and methods of making and using such polymers are disclosed. Also disclosed are dibenzosilole derivatives substituted with one or more water-solubilizing groups, and methods of making and using such derivatives to prepare water-soluble dibenzosilole polymers.. ... Life Technologies Corporation

08/31/17 / #20170246603

Gas spargers and related container systems

A container system includes a collapsible bag having an interior surface at least partially bounding a chamber, the chamber being adapted to hold a fluid. A sparger is disposed within the chamber of the bag, the sparger bounding a compartment and having an inside edge that encircles an opening passing through the sparger. ... Life Technologies Corporation

08/24/17 / #20170241943

Methods for calibrating an array of chemically-sensitive sensors

Methods and apparatus relating to very large scale fet arrays for analyte measurements. Chemfet (e.g., isfet) arrays may be fabricated using conventional cmos processing techniques based on improved fet pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. ... Life Technologies Corporation

08/17/17 / #20170235874

Methods and systems for detecting minor variants in a sample of genetic material

A computer-implemented method for determining minor variants. The method includes receiving electropherogram sequence data from a test sample, identifying any non-primary peaks in the electropherogram, and characterizing identified non-primary peaks using at least one signal feature. ... Life Technologies Corporation

08/10/17 / #20170225169

Thermal uniformity for thermal cycler instrumentation using dynamic control

A method for performing polymerase chain reactions (pcr) for improving thermal non-uniformity is provided. The method includes measuring a first temperature, by a first sensor, of a first sample block sector of a sample block and measuring a second temperature, by a second sensor, of a second sample block sector of the sample block that is adjacent to the first sample block sector. ... Life Technologies Corporation

08/03/17 / #20170218095

Monodisperse submicron polymer particles

This invention relates to monodisperse cross-linked polymer particles, comprising particles with a substantially smooth outer surface and an average diameter of less than 1 μm, wherein the particles are solid or porous, and wherein the coefficient of variation (cv) % of the particles, when measured by cps disk centrifugation analysis, is less than 15%. These monodisperse cross-linked polymer particles may comprise magnetic material and are useful in various application. ... Life Technologies Corporation

07/20/17 / #20170206313

Using flow space alignment to distinguish duplicate reads

Systems and method for identifying duplicate reads can receive first and second reads, determine if the first and second reads have a same start and end position, determine a binary flow difference, and identify the second read as a duplicate of the first read when the binary flow difference exceeds a threshold.. . ... Life Technologies Corporation

07/20/17 / #20170204398

Nucleic acid preparation method

A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support whilst maintaining it on the support. ... Life Technologies Corporation

07/13/17 / #20170199734

Systems and methods for versioning hosted software

Systems and methods for performing a validated analysis can include access to first and second versions of an analysis engine. The first version of the analysis engine can be used to perform a previously validated analysis. ... Life Technologies Corporation

07/13/17 / #20170197185

Container with film sparger

A container assembly includes a flexible bag having an interior surface bounding a chamber and an opposing exterior surface, the bag having a bottom end wall being comprised of a first sheet overlying a second sheet, the first sheet and the second sheet of the bottom end wall being welded together so as to form a first sparging area and a separated second sparging area that are both bounded between the first sheet and the second sheet, at least a portion of the first sheet overlying the first and second sparging areas being gas permeable.. . ... Life Technologies Corporation

07/06/17 / #20170194985

Methods and computer program products for compression of sequencing data

A compression method includes measuring a waveform associated with a chemical event occurring on a sensor array, wherein the waveform comprises at least one region associated with expected measured values and at least one region associated with unpredictable measured values; applying a first compression process to the waveform, the first compression process including an averaging of one or more frames in one or more portions of the waveform; applying a keyframe delta compression to the frame-averaged data; forming a compressed data structure including a keyframe and calculated differences subsequent to the keyframe; and storing one or more compressed data structures in memory.. . ... Life Technologies Corporation

07/06/17 / #20170191922

System and method for providing stable fluid flow

An embodiment of a system with a minute measure of pulsatility in a flow of a fluid is described that comprises a first pump configured to flow the fluid to a junction at a first flow rate that comprises a measure of pulsatility; and a second pump configured to flow a portion of the fluid from the junction at a second flow rate that is less than the first flow rate to produce a flow of the fluid at a third flow rate from the junction with a minute measure of pulsatility.. . ... Life Technologies Corporation

07/06/17 / #20170191571

Pinch flow regulator

A valve for regulating fluid flow includes a housing base defining a lower cavity and comprising a pinch structure within the lower cavity, a gas inlet providing external access to the lower cavity, a base fluid inlet, and a base fluid outlet. A housing cover defines an upper cavity and comprises a cover fluid inlet and a cover fluid outlet. ... Life Technologies Corporation

07/06/17 / #20170191051

Labeled enzyme compositions, methods and systems

Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are labeled polymerase conjugates including a polymerase linked to one or more labels, wherein the conjugate has polymerase activity. ... Life Technologies Corporation

06/29/17 / #20170186840

Nanocrystals with high extinction coefficients and methods of making and using such nanocrystals

A population of bright and stable nanocrystals is provided. The nanocrystals include a semiconductor core and a thick semiconductor shell and can exhibit high extinction coefficients, high quantum yields, and limited or no detectable blinking.. ... Life Technologies Corporation

06/29/17 / #20170183617

Fluid mixing system with laterally displaced flexible drive lines and methods of use

A fluid mixing system includes a container bounding a compartment and extending between a first end and an opposing second end. An elongated first drive line and second drive line are disposed within the compartment of the container and are rotatable therein. ... Life Technologies Corporation

06/29/17 / #20170182496

Systems and methods for transfer of liquid samples

A system for preparing biological sample contains a body including a proximal side and a distal side, a plurality of mandrels, a plurality of resilient elements, a plurality of fluid dispensers, and one or more samples. The mandrels are moveably positioned within the body, where each resilient element engages a respective one of the mandrels. ... Life Technologies Corporation

06/29/17 / #20170182492

Integrated system for nucleic acid amplification and detection

Devices, systems, methods, and kits are disclosed for amplifying small quantities of nucleic acid and identifying the nucleic acid sequences of the amplified nucleic acid products.. . ... Life Technologies Corporation

06/22/17 / #20170177792

Methods and systems for knowledge discovery using biological data

A system for analyzing biological data, comprising: a storage configured to store a plurality of data files containing biological data obtained from a plurality of devices; a server configured to: host a plurality of applications, each configured to be implemented on the server and to provide analysis, manipulation, comparison, visualization, or a combination thereof, of the biological data included in the data files, wherein the plurality of applications allow a user to analyze different data files related to the same sample and compare the results of the analysis.. . ... Life Technologies Corporation

06/22/17 / #20170177791

Methods and systems for pcr quantitation

A method for quantifying nucleic acid is provided. The method includes determining a first reference threshold cycle for a first predetermined input quantity for a reference nucleic acid, determining a first target threshold cycle for the first predetermined input quantity for a target nucleic acid, determining a second reference threshold cycle for a second predetermined input quantity for the reference nucleic acid, and determining a second target threshold cycle, by the processor, for the second predetermined input quantity for the target nucleic acid. ... Life Technologies Corporation

06/22/17 / #20170176385

Capillary array cartridge for capillary electrophoresis systems

The present disclosure relates, in some embodiments, to an apparatus for conducting a capillary electrophoresis assay. The apparatus can comprise a capillary array comprising an anode end and a cathode end, the capillary array provided in a housing further comprising a reservoir configured to house a separation medium and an anode buffer. ... Life Technologies Corporation

06/22/17 / #20170176384

Apparatuses, systems and methods for sequencing using capillary electrophoresis

In one aspect, a biological sequencing device comprising a cartridge configured to be removed from the instrument is disclosed. In various embodiments the cartridge can include one or more capillaries suitable for capillary electrophoresis, a reservoir and a pump. ... Life Technologies Corporation

06/22/17 / #20170175171

Methods and kits for breaking emulsions

The disclosure relates generally to methods, kits and systems for extracting biomolecules from a water-in-oil emulsion, including breaking a water-in-oil emulsion comprising a plurality of aqueous droplets in a continuous hydrophobic fraction using a breaking solution to produce a resulting reaction mixture containing one or more biomolecules and manipulating the resulting reaction mixture to form at least two phases, where one of the phases includes an aqueous phase containing the one or more biomolecules.. . ... Life Technologies Corporation

06/22/17 / #20170175092

Mutant rb69 dna polymerase

Provided herein are mutant dna-dependent polymerases which are derived from, or otherwise related to, wild type rb69 dna polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. ... Life Technologies Corporation

06/22/17 / #20170174846

Purification systems and methods

A method of preparing hydrogel particles includes applying a solution including a plurality of hydrogel particles to a stir cell. A retentate side of a filter defines a lower surface of the stir cell. ... Life Technologies Corporation








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