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Life Technologies Corporation patents (2015 archive)


Recent patent applications related to Life Technologies Corporation. Life Technologies Corporation is listed as an Agent/Assignee. Note: Life Technologies Corporation may have other listings under different names/spellings. We're not affiliated with Life Technologies Corporation, we're just tracking patents.

ARCHIVE: New 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 | Company Directory "L" | Life Technologies Corporation-related inventors


12/31/15 / #20150376721

Direct quantitative pcr absent minor groove binders

Disclosed herein are methods, compositions and kits for the quantification of a nucleic acid target present on a solid support. This entails quantitative real-time polymerase chain reaction wherein minor groove binders are excluded.. ... Life Technologies Corporation

12/31/15 / #20150376687

Direct quantitative pcr absent minor groove binders

Disclosed herein are methods, compositions and kits for the quantification of a nucleic acid target present on a solid support. This entails quantitative real-time polymerase chain reaction wherein minor groove binders are excluded.. ... Life Technologies Corporation

12/24/15 / #20150369828

System and method incorporating solid buffer

A buffered suspension includes a surfactant and a solid buffer particulate having a point of zero charge at least 1.2 ph units different that the ph of the buffered suspension. The buffered suspension can be prepared by mixing a stock solution with the solid buffer particulate and titrating. ... Life Technologies Corporation

12/24/15 / #20150369728

Two-dimensional spectral imaging system

Systems, including methods, apparatus, and algorithms, for spectrally imaging a two-dimensional array of samples.. . ... Life Technologies Corporation

12/24/15 / #20150368626

Polymerase compositions, methods of making and using same

The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragment thereof are provided that allow for nucleic acid amplification. ... Life Technologies Corporation

12/17/15 / #20150363586

Systems and methods for identifying an individual

The present application relates to systems and methods using biometric data of an individual for identifying the individual and/or verifying the identity of an individual. These systems and methods are useful for, amongst many applications, more secure identification of high-risk individuals attempting to gain access to an entity, transport, information, location, security organization, law enforcement organization, transaction, services, authorized status, and/or funds.. ... Life Technologies Corporation

12/17/15 / #20150361488

Sequencing device

A method of preparing reagents includes inserting a cartridge into an instrument. The cartridge includes a plurality of reagent enclosures disposed in a cavity of the cartridge and exposing a port to an exterior of the cartridge. ... Life Technologies Corporation

12/17/15 / #20150361481

Multiplex nucleic acid amplification

In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. ... Life Technologies Corporation

12/17/15 / #20150361418

Systems and methods for substrate enrichment

A method of separating bead substrates includes applying an emulsion to an emulsion-breaking solution. A dispersed phase of the emulsion includes an unbound polynucleotide, a first set of bead substrates and a second set of bead substrates. ... Life Technologies Corporation

12/17/15 / #20150360227

Pinch flow regulator

A valve for regulating fluid flow includes a housing base defining a lower cavity and comprising a pinch structure within the lower cavity, a gas inlet providing external access to the lower cavity, a base fluid inlet, and a base fluid outlet. A housing cover defines an upper cavity and comprises a cover fluid inlet and a cover fluid outlet. ... Life Technologies Corporation

12/17/15 / #20150360221

Reagent mixer and fluid control devices

An apparatus for preparing a reagent solution includes an enclosure and a container disposed within the enclosure. The container defines an internal cavity having a compressible volume and defines a passage providing fluidic communication between the internal cavity and the exterior of the container. ... Life Technologies Corporation

12/10/15 / #20150356242

Systems and methods for gene expression analysis

. . The present disclosure provides systems, methods, and a computer-readable storage medium for identifying and selecting highly relevant sets of genes (or gene signatures and biomarkers), which, when coupled with clinical and/or demographic data are highly predictive of potential clinical responses to a targeted therapy. It also provides systems, methods, and a computer-readable storage medium for determining the expected response to the targeted therapy when using the selected gene/biomarker set.. ... Life Technologies Corporation

12/10/15 / #20150356241

Methods and systems for determining meta-genotypes

In one exemplary embodiment, a computer-implemented method for determining a genetic result from a biological sample is provided. The method includes receiving nucleic acid amplification data of a biological sample, by a processor, from a biological instrument. ... Life Technologies Corporation

12/10/15 / #20150355269

Method and apparatus for identifying defects in a chemical sensor array

In one implementation, a method for operating an apparatus is described. The method includes applying a bias voltage to place a transistor of a reference sensor in a known state, the reference sensor in an array of sensors that further includes a chemical sensor coupled to a reaction region for receiving at least one reactant. ... Life Technologies Corporation

12/10/15 / #20150355265

Method and apparatus for identifying defects in a chemical sensor array

An apparatus including an array of sensors including a plurality of chemical sensors and a plurality of reference sensors, each chemical sensor coupled to a corresponding reaction region for receiving at least one reactant, and each reference sensor comprising a field effect transistor having a gate coupled to a corresponding reference line and an access circuit for accessing the chemical sensors and the reference sensors and a controller to apply bias voltages to the reference lines to select corresponding reference sensors, acquire output signals from the selected reference sensors, and identify one or more defects in the access circuit based on differences between the acquired output signals and expected output signals.. . ... Life Technologies Corporation

12/10/15 / #20150355138

Methods, systems, and computer-readable media for compression of sequencing data

Methods, systems, and computer-readable media are disclosed for compression of sequencing data. One method includes receiving waveform data associated with a chemical event occurring on a sensor array, the waveform data including a plurality of time-based waveforms of a corresponding plurality of locations of the sensor array; converting, by at least one processor, each time-based waveform of the waveform data into a frequency-domain spectrum; generating, by the at least one processor, a key frame based on a plurality of the frequency-domain spectrums; calculating, by the at least one processor, for each of the frequency-domain spectrums, a difference between the frequency-domain spectrum and the key frame; and encoding, by the at least one processor, each calculated difference between the frequency-domain spectrum and the key frame.. ... Life Technologies Corporation

12/10/15 / #20150355079

Spatial positioning of spectrally labeled beads

Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. ... Life Technologies Corporation

12/10/15 / #20150354015

Method for direct amplification from crude nucleic acid samples

The present teachings relate to improved methods, kits, and reaction mixtures for amplifying nucleic acids. In some embodiments a novel direct buffer formulation is provided which allows for the direct amplification of the nucleic acids in a crude sample with minimal sample purification.. ... Life Technologies Corporation

12/10/15 / #20150353994

Bi-directional sequencing compositions and methods

In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, in some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.. . ... Life Technologies Corporation

12/10/15 / #20150353993

Compositions, methods, systems and kits for target nucleic acid enrichment

The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the concentration of target nucleic acid molecules from a sample. ... Life Technologies Corporation

12/03/15 / #20150344938

Multiplex transcriptome analysis

In some embodiments, the disclosure relates generally to methods, compositions, systems, apparatuses and kits comprising a multiplex nucleic acid amplification reaction that employs a plurality (e.g., hundreds, thousands, tens-of-thousands or hundreds-of-thousands) of different target-specific primer pairs that enable substantially simultaneous amplification of a plurality of different target sequences-of-interest in a single reaction mixture. In some embodiments, the multiplex nucleic acid amplification reaction generates a plurality of amplicons having sequences derived from a sample containing rna or dna, including whole transcriptome or genomic samples. ... Life Technologies Corporation

11/26/15 / #20150337300

Methods and apparatuses for nucleic acid shearing by sonication

Methods and kits for preparing nucleic acid fragments from a sample of purified nucleic acid are provided. Alternatively, chromatin or other long polymers can be sheared with similar methods and kits.. ... Life Technologies Corporation

11/26/15 / #20150337299

Methods and apparatuses for nucleic acid shearing by sonication

Methods and kits for preparing nucleic acid fragments from a sample of purified nucleic acid are provided. Alternatively, chromatin or other long polymers can be sheared with similar methods and kits.. ... Life Technologies Corporation

11/19/15 / #20150329904

Methods and apparatus for measuring analytes using large scale fet arrays

Methods and apparatus relating to very large scale fet arrays for analyte measurements. Chemfet (e.g., isfet) arrays may be fabricated using conventional cmos processing techniques based on improved fet pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. ... Life Technologies Corporation

11/19/15 / #20150329899

Systems and methods for validation of sequencing results

Systems and method for validation of sequencing results can amplify a target region of a nucleic acid sample in the presence of a primer pool including target specific and variant specific primers. The variant specific primers can include variant specific barcodes and variant specific sequences. ... Life Technologies Corporation

11/19/15 / #20150328640

Vertical clamp device

A vertical clamping device is provided that supports a flow cell component in a vertical configuration in which the flow cell is on an opposite side of the vertical support from the electronic interface. The clamp includes a vertical setting to receive the flow cell component and provides an electronic interface on a vertical surface of the vertical setting. ... Life Technologies Corporation

11/19/15 / #20150328634

Case for containing biological samples and corresponding method of use

A case for containing biological samples includes a base, a substrate, and a cover. The substrate includes a plurality of reaction regions disposed along a surface of the substrate and configured to receive one or more biological samples. ... Life Technologies Corporation

11/12/15 / #20150322694

Self-locking door and product dispensing enclosure having a self-locking door

Provided herein is an inventory management system for dispensing products from an enclosure, methods of securing products within the enclosure, use of the enclosure and for maintaining inventory. The inventory management system may include an enclosure having a self-locking door. ... Life Technologies Corporation

11/12/15 / #20150322522

Novel human single nucleotide polymorphisms

Disclosed are methods for human identification utilizing newly discovered single nucleotide polymorphisms (snps) within codis loci which can cause allelic dropout. Also disclosed are kits useful in human identification.. ... Life Technologies Corporation

11/12/15 / #20150322504

Sequence amplification with loopable primers

The present disclosure relates to the amplification of target nucleic acid sequences. This can be accomplished via the use of various primers. ... Life Technologies Corporation

11/12/15 / #20150322490

Methods and compositions for multiplex pcr

The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex pcr of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. ... Life Technologies Corporation

11/12/15 / #20150322488

Instrument for monitoring polymerase chain reaction of dna

An optical instrument monitors pcr replication of dna in a reaction apparatus having a temperature cycled block with vials of reaction ingredients including dye that fluoresces in presence of double-stranded dna. A beam splitter passes an excitation beam to the vials to fluoresce the dye. ... Life Technologies Corporation

11/05/15 / #20150317509

Interactive and automated tissue image analysis with global training database and variable-abstraction processing in cytological specimen classification and laser capture microdissection applications

A system and method for performing tissue image analysis and region of interest identification for further processing applications such as laser capture microdissection is provided. The invention provides three-stage processing with flexible state transition that allows image recognition to be performed at an appropriate level of abstraction. ... Life Technologies Corporation

11/05/15 / #20150316558

Fluorescent chemical compounds having high selectivity for double stranded dna, and methods for their use

Chemical compounds having a high selectivity for double stranded dna over rna and single stranded dna are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded dna, but exhibit reduced or no fluorescence in the absence of double stranded dna. ... Life Technologies Corporation

11/05/15 / #20150315657

Gene fusions and gene variants associated with cancer

The disclosure provides gene fusions, gene variants, and novel associations with disease states, as well as kits, probes, and methods of using the same.. . ... Life Technologies Corporation

11/05/15 / #20150314294

System and methods for making and processing emulsions

An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. ... Life Technologies Corporation

10/29/15 / #20150307929

Methods and compositions for multiplex pcr

The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex pcr of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. ... Life Technologies Corporation

10/29/15 / #20150307920

Methods and compositions for multiplex pcr

The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex pcr of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. ... Life Technologies Corporation

10/29/15 / #20150307901

Molecular biology tools for algal engineering

The present invention provides compositions and methods for the genetic manipulation of algal cells. The compositions and methods allow enhanced transfer of genetic material into algal cells and the cloning and selection of genetically modified cells. ... Life Technologies Corporation

10/29/15 / #20150307869

Device for capture and lysis of microorganisms from liquids and methods of use thereof

Devices and methods for detecting microbial contaminants, such as bacteria and fungi, in fluids such as drinking water, pharmaceutical solutions and tissue culture media are provided. More particularly, provided are filtration devices for capture and processing of microorganisms from fluids, and improved methods for recovery, lysis and detection of microorganisms based on a combination of physical disruption with small beads and lysis solutions.. ... Life Technologies Corporation

10/29/15 / #20150306166

Antimicrobial & antiviral composition

A method of reducing the number of microorganisms entering the nose and proliferating in the nasal cavity including application of a solution of an antimicrobial, antiviral and antifungal composition to the anterior vestibular region of the nares. The antimicrobial, antiviral and antifungal solution includes ethyl alcohol as an active ingredient. ... Life Technologies Corporation

10/22/15 / #20150300873

Load cell overload protection by a relieving mechanism for fluid dispensing systems

A method for locking out load cells includes dispensing a fluid into a collapsible bag that is supported within a support housing, the support housing resting on a plurality of load cells that sense the weight of the fluid; manipulating a plurality of lockouts located adjacent to corresponding load cells from a first position to a second position so that the lockouts remove at least a portion of the weight of the fluid from the plurality of load cells; moving the support housing containing the collapsible bag with the fluid therein to a new location; and further manipulating the plurality of lockouts from the second position back to the first position so that the plurality of load cells again sense the full weight of the fluid within the collapsible bag.. . ... Life Technologies Corporation

10/22/15 / #20150299787

Immobilized buffer particles and uses thereof

The disclosure relates to novel particle compositions and methods of making said compositions having applications in nucleic acid analysis, as well as apparatuses and systems for the same.. . ... Life Technologies Corporation

10/01/15 / #20150278904

Collections of matched biological reagents and methods for identifying matched reagents

Provided herein are collections of matched biological reagents selected from a larger collection of biological reagents, wherein the collection of matched biological reagents relate to a biological element. Also provided are methods for selling an isolated biomolecule or biological research reagent in a collection of matched biological reagents, and methods for selecting an isolated biomolecule or biological research reagent from a collection of biological reagents.. ... Life Technologies Corporation

10/01/15 / #20150278437

Methods and systems for a digital pcr experiment designer

A computer-implemented method for designing a digital pcr (dpcr) experiment is provided. The method includes receiving, from a user, a selection of optimization type. ... Life Technologies Corporation

10/01/15 / #20150275284

Clonal amplification of nucleic acid on solid surface with template walking

Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.. . ... Life Technologies Corporation

10/01/15 / #20150275257

Nucleic acid amplification

The present invention provides improved methods for the amplification of nucleic acid molecules. Methods for amplifying target polynucleotides, including mrna, using oligonucleotides, dna and rna polymerases are provided. ... Life Technologies Corporation

09/24/15 / #20150269756

Visualization tools for digital pcr data

A method for generating a data visualization is provided. The method includes displaying a representation of a portion of detected data from a substrate to a user. ... Life Technologies Corporation

09/24/15 / #20150267264

Analysis of y-chromosome str markers

The methods and compositions provided herein relate to the discovery of 13 str markers, found on the human y chromosome, having surprisingly high mutation rates when compared with 173 other y-str markers known today. The set of rm-y-strs may overcome the current dilemma of y-chromosome analysis in forensic applications due to their extraordinary mutation properties. ... Life Technologies Corporation

09/24/15 / #20150267209

System and method for visualization of optimized protein expression

The present disclosure describes a novel recombinant gene expression system and a method of its use. The recombinant gene expression system is adapted to enable a user to monitor the successful expression of a protein in real time by allowing a small portion (e.g., less than 10%) of the target protein to be expressed as a fusion with a readily detectable reporter. ... Life Technologies Corporation

09/24/15 / #20150265988

Impeller assemblies for fluid processing systems

A fluid mixing system includes a flexible bag having an interior surface bounding a compartment. An elongated first tubular connector is disposed within the compartment of the flexible bag, the first tubular connector having a first end and an opposing second end, the first end of the first tubular connector being coupled with the flexible bag. ... Life Technologies Corporation

09/24/15 / #20150265958

Gas filter systems for fluid processing systems

A filter assembly includes a casing having of a polymeric film and bounding a compartment, the casing having an inlet opening communicating with the compartment and a first outlet opening communicating with the compartment. A first filter includes a porous filter body through which gas can pass and has a pore size smaller than 1 μm, the first filter being coupled with the casing so that the filter body is at least partially disposed within compartment of the casing and so that gas passing through the compartment of the casing from the inlet opening to the first outlet opening must pass through the filter body.. ... Life Technologies Corporation

09/24/15 / #20150265943

Condenser systems for fluid processing systems

A condenser bag includes a body bounding a channel that extends between an inlet opening at a first end and an outlet opening and an opposing second end, the body being comprised of a polymeric film. An intake port is secured to the body at the inlet opening and bounds an port opening that extends therethrough and communicates with the channel. ... Life Technologies Corporation

09/17/15 / #20150260680

Modified proteins and methods of making and using same

Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pka values are described. ... Life Technologies Corporation

09/17/15 / #20150259082

Fluid manifold systems

A fluid manifold system includes a manifold having at least portions of opposing flexible sheets welded together to form a fluid flow path therebetween, a fluid inlet communicating with the fluid flow path. A plurality of receiving containers are in fluid communication with the fluid flow path of the manifold, each receiving container bounding a compartment. ... Life Technologies Corporation

09/10/15 / #20150253235

Systems and methods for diagnosing a fluidics system and determining data processing settings for a flow cytometer

The present set of embodiments relates to systems and methods for diagnosing a fluidics system and determining data processing settings for a flow cytometer. Systems and methods for diagnosing a fluidics system require accurate measurement and interpretation of fluctuations within the fluid delivery system. ... Life Technologies Corporation

09/10/15 / #20150252442

Interference control panel for evaluation of analytical assays for samples derived from blood

The invention relates to quality control of analytical assays, particularly nat assays of blood samples containing nucleic acids. A control panel containing quantified amounts of substances known to interfere with an analytical assay is used and compared with a reference sample in the analytical assay. ... Life Technologies Corporation

09/10/15 / #20150252406

Systems and methods to control dissolved gas

A method of sensing nucleotide reactions includes flowing at least one nucleotide solution from a container of at least two containers of a sensor system. The sensor system includes a sensor sensitive to a byproduct of nucleotide incorporation. ... Life Technologies Corporation

09/10/15 / #20150252373

Alkynyl-derivatized cap analogs, preparation and uses thereof

Alkynyl-derivatized cap analogs, alkynyl-modified capped rna, 1,4-disubstituted triazole-derivatized capped rna, methods of preparation, methods of isolation, and uses thereof are provided. The “click” modification facilitates detection and isolation of capped rnas and the 1,4-disubstituted triazole derivatives formed by the “click” reaction are useful for producing rna transcripts and encoded protein.. ... Life Technologies Corporation

09/03/15 / #20150247863

Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings

The present invention describes novel dyes, including coumarins, rhodamines, and rhodols that incorporate additional fused aromatic rings. The dyes of the invention absorb at a longer wavelength than structurally similar dyes that do not possess the fused aromatic rings. ... Life Technologies Corporation

09/03/15 / #20150247852

Markers capable of distinguishing cell pluripotency and uses thereof

Provided herein are compositions, methods and uses that relate to or result from the identification of markers that can distinguish between cells at different stages of pluripotency. Certain embodiments provide markers that can distinguish between parental cells (i.e. ... Life Technologies Corporation

09/03/15 / #20150247801

Spatial positioning of spectrally labeled beads

Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. ... Life Technologies Corporation

09/03/15 / #20150247799

Detection of immobilized nucleic acid

The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded dna, rna or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. ... Life Technologies Corporation

09/03/15 / #20150247789

Systems, methods, and apparatuses for optical systems in flow cytometers

The present set of embodiments relate to a system, method, and apparatus for an optical configuration in a flow cytometer that allows for independent adjustment of focusing for each light source. Such systems, methods, and apparatuses require a final focusing element to be moved near the beginning of the optical train and for each optical element coming after the final focusing element to be configured to accommodate converging light beams while minimizing the introduction of aberrations into those beams.. ... Life Technologies Corporation

09/03/15 / #20150247785

Modified carbocyanine dyes and their conjugates

Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. ... Life Technologies Corporation

09/03/15 / #20150247136

Polymerase compositions and methods

Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. ... Life Technologies Corporation

09/03/15 / #20150246146

Methods and compositions for enzyme-mediated site-specific radiolabeling of glycoproteins

Provided herein are methods, compositions and kits for use in the site-specific labeling of glycoproteins comprising a combination of enzyme-mediated incorporation of modified sugars comprising a chemical handle and cycloaddition chemistry with a labeling molecule comprising a reactive group, a metal ion chelator, and/or a fluorophore.. . ... Life Technologies Corporation

08/27/15 / #20150241438

Fluorogenic ph-sensitive dyes and their methods of use

Disclosed herein are compounds, compositions, methods and kits for detecting ph in samples using ph-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting ph in samples either in vitro or in vivo. ... Life Technologies Corporation

08/27/15 / #20150241387

Chemical detection device having multiple flow channels

The described embodiments may provide a chemical detection circuit that may comprise a plurality of first output circuits at a first side and a plurality of second output circuits at a second side of the chemical detection circuit. The chemical detection circuit may further comprise a plurality of tiles of pixels each placed between respective pairs of first and second output circuits. ... Life Technologies Corporation

08/20/15 / #20150233936

Water-dispersable nanoparticles

Provided herein are methods for making water-soluble nanoparticles comprising a core/shell nanocrystal that is coated with a surface layer comprising enough hydrophilic ligands to render the nanoparticle water soluble or water dispersable. Methods for crosslinking molecules on the surface of a nanoparticle, which methods can be used on the above water-soluble nanoparticles also are provided. ... Life Technologies Corporation

08/20/15 / #20150231636

Apparatuses, systems and methods for providing scalable thermal cyclers and isolating thermoelectric devices

In one aspect, a thermal cycler system including a sample block and a thermoelectric device is disclosed. In various embodiments, the sample block has a first surface configured to receive a plurality of reaction vessels and an opposing second surface. ... Life Technologies Corporation

08/13/15 / #20150225776

Gene expression profiling from ffpe samples

Methods and compositions relating to the generation and use of gene expression data from tissue samples that have been fixed and embedded are provided. The data can electronically stored and implemented as well as used to augment diagnosis and treatment of diseases.. ... Life Technologies Corporation

08/13/15 / #20150225774

Methods, workflows, kits, apparatuses, and computer program media for nucleic acid sample preparation for nucleic acid sequencing

A method for preparing a nucleic acid sample for nucleic acid sequencing includes amplifying a nucleic acid target sequence using a primer bound to a first capture substrate; capturing an amplified nucleic acid product by the first capture substrate; generating at least one sequencing ladder from the amplified nucleic acid product using at least one sequencing primer; capturing the at least one sequencing ladder by hybridizing the at least one sequencing ladder to a complementary capture compound on a second capture substrate; and removing the at least one sequencing ladder from the second capture substrate. The first and/or second capture substrate may include a magnetic particle. ... Life Technologies Corporation

08/13/15 / #20150225729

Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites

The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. ... Life Technologies Corporation

08/13/15 / #20150224457

System and methods for making and processing emulsions

An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion pcr (epcr) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. ... Life Technologies Corporation

08/06/15 / #20150221102

Virtual cellular staining

Systems and methods are used to display cell structures of a biological cell. A plurality of cell structures of a biological cell is stored and for each cell structure of the plurality of cell structures one or more stain colors are stored. ... Life Technologies Corporation

08/06/15 / #20150219634

Use of novel coumarins as glutathione and thiol labels

Fluorescent quinolizinocoumarin compounds substituted with electrophilic reactive groups that bind thiol compounds are described. The compounds are useful in detecting oxidative stress and processes associated therewith in live cells.. ... Life Technologies Corporation

08/06/15 / #20150218630

Dna sequencing methods and detectors and systems for carrying out the same

In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanofet devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. ... Life Technologies Corporation

08/06/15 / #20150218513

Hepatocyte preparations

Methods for preparing a variety of cell (e.g., hepatocyte) preparations and preparations so prepared are described. Uses of such preparations are described. ... Life Technologies Corporation

08/06/15 / #20150218380

Modified carbocyanine dyes and their conjugates

Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. ... Life Technologies Corporation

08/06/15 / #20150218379

Fluorogenic ph-sensitive dyes and their methods of use (ii)

Disclosed herein are compounds, compositions, methods and kits for detecting ph in samples using ph-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting ph in samples either in vitro or in vivo. ... Life Technologies Corporation

08/06/15 / #20150217292

Apparatus and method for segmented thermal cycler

The present invention relates to a thermal cycler for the carrying out of chemical or biological reactions, such as pcr or other nucleic acid amplification reactions, that is segmented with a plurality of reaction vessel receiving elements. The reaction vessel receiving elements are thermally isolated from each other and provide an airtight seal to prevent liquids or moisture from penetrating below the reaction vessel receiving elements. ... Life Technologies Corporation

08/06/15 / #20150216662

Biological chord repair system and methods

An embodiment includes a chordal replacement system comprising: a pledget coupled to a first suture length and at least one of a first needle and a first ferrule; a second suture length coupled to the pledget and at least a second needle; and a third suture length coupled to the pledget and at least a third needle; wherein the second and third suture lengths are not monolithic with each other and do not constitute a single suture. Other embodiments are described herein.. ... Life Technologies Corporation

07/30/15 / #20150211051

Method for recovering sperm nucleic acid from a forensic sample

A method for selectively recovering nucleic acid from a sperm cell in a sample containing cells of at least a sperm cell and an epithelial cell, and a cell suspension medium comprising extracellular impurities, is provided. The method entails introducing a sample into a vessel, sequestering the cells from the remaining sample components, washing the cells with a washing solution either before or after sequestration, removing the impurities-containing cell suspension medium from the vessel while retaining the cells; lysing selectively cells of the first cell type; and isolating the nucleic acid from the lysed cells. ... Life Technologies Corporation

07/30/15 / #20150211021

Membrane-penetrating peptides to enhance transfection and compositions and methods for using same

The present invention is directed to non-naturally occurring peptides containing a membrane-penetrating amino acid sequence and further at least one polycationic moiety or peptide sequence. The peptides are suitable for use in delivery a cargo to the interior of a cell. ... Life Technologies Corporation

07/30/15 / #20150210989

Novel reverse transcriptases for use in high temperature nucleic acid synthesis

The invention provides novel reverse transcriptases (rts) with desirable properties such as increased thermostability, increased thermoreactivity and/or increased resistance to inhibitors. In certain embodiments, the invention provides methods of producing, amplifying and/or sequencing nucleic acid molecules (particularly cdna molecules) using kits, compositions and/or reactions mixtures containing such novel reverse transcriptase enzymes.. ... Life Technologies Corporation

07/23/15 / #20150206762

Methods and systems for point of use removal of sacrificial material

A method of manufacturing a sensor, the method including forming an array of chemically-sensitive field effect transistors (chemfets), depositing a dielectric layer over the chemfets in the array, depositing a protective layer over the dielectric layer, etching the dielectric layer and the protective layer to form cavities corresponding to sensing surfaces of the chemfets, and removing the protective layer. The method further includes, etching the dielectric layer and the protective layer together to form cavities corresponding to sensing surfaces of the chemfets. ... Life Technologies Corporation

07/23/15 / #20150204815

Liquid protein markers for native gel electrophoresis

Marker sets are provided for use on nondenaturing gels. The protein molecular weight markers are provided in liquid form, and are stable in liquid form for at least two months at 4 degrees c. ... Life Technologies Corporation

07/23/15 / #20150203912

Protein detection using fet

Accordingly, in some embodiments methods for detecting an analyte or analytes in one or more sample(s) are provided. The methods encompass providing a solid support with an addressable marker and an associated ligand, contacting the solid substrate to a sample, thereby forming a contacted solid support, associating the contacted solid support with a fet array and detecting the electrical properties of the fet array and thereby detecting an analyte or analytes in one or more samples. ... Life Technologies Corporation

07/23/15 / #20150203901

Compositions and methods for detection of salmonella species

Described are compositions, methods and kits for detection and/or differential detection of serovars of salmonella enterica subsp. Enterica serovar such as s. ... Life Technologies Corporation

07/16/15 / #20150198606

Method for enhancing transport of semiconductor nanocrystals across biological membranes

Semiconductor nanoparticle complexes comprising semiconductor nanoparticles in association with cationic polymers are described. Also described are methods for enhancing the transport of semiconductor nanoparticles across biological membranes to provide encoded cells. ... Life Technologies Corporation

07/16/15 / #20150198549

Reactor foam sensor systems and methods of use

A foam sensor system includes a flexible bag comprised of a polymeric material and bounding a compartment. A foam sensor assembly is mounted on the flexible bag and includes a foam contact disposed within the compartment of the flexible bag. ... Life Technologies Corporation

07/16/15 / #20150197797

Integrated sensor arrays for biological and chemical analysis

An apparatus comprising a chemical field effect transistor array in a circuit-supporting substrate is disclosed. The transistor array has disposed on its surface an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid. ... Life Technologies Corporation

07/16/15 / #20150197743

Magnetic beads having surface glycoconjugates and use thereof

Magnetic beads that include polyvalent ligands comprising various carbohydrates are described. Methods for fabricating such magnetic beads are also provided as well as methods of their use to capture and enrich pathogen cell population for subsequent culture, lysis and identification.. ... Life Technologies Corporation

07/09/15 / #20150191784

Fast-indexing filter wheel and method of use

. . . . Various embodiments of a sequencing system capable of rapidly imaging samples at multiple wavelengths are provided herein. In one embodiment, the system includes a fast-indexing filter wheel having a plurality of excitation and emission filters capable of being rapidly rotated into and out of communication with an excitation source (e.g., an arc lamp, a laser. ... Life Technologies Corporation

07/09/15 / #20150191781

Random-primed transcriptase in-vitro transcription method for rna amplification

A random-primed reverse transcriptase-in vitro transcription method of linearly amplifying rna is provided. According to the methods of the invention, source rna (or other single-stranded nucleic acid), preferably, mrna, is converted to double-stranded cdna using two random primers, one of which comprises a rna polymerase promoter sequence (“promoter-primer”), to yield a double-stranded cdna that comprises a rna polymerase promoter that is recognized by a rna polymerase. ... Life Technologies Corporation

07/09/15 / #20150191778

Compositions and methods for detecting and identifying salmonella enterica strains

The present specification describes several novel snps of salmonella enterica subsp. Enterica. ... Life Technologies Corporation

07/09/15 / #20150191775

Polymerization of nucleic acids using activation by polyphosphorolysis (app) reactions

This disclosure relates to methods of performing activation by polyphosphorolysis (app) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or μ-monothiopyrophosphate), and related compounds.. . ... Life Technologies Corporation

07/09/15 / #20150191774

Composition and method for sequencing nucleic acid

A composition for sequencing dna is provided and comprises a nuclease and a nuclease-resistant sequencing primer. A method of preparing dna for sequencing and a method of sequencing dna are also provided. ... Life Technologies Corporation

07/09/15 / #20150190765

Automated liquid manufacturing system

A method for continuously preparing a medium formulation mixes a diluent with a plurality of chemically incompatible concentrate solutions in such a manner that none of the ingredients of the concentrate solutions chemically react in an adverse manner. The method utilizes a static mixing chamber to add the concentrate solutions to the diluent stream sufficiently in advance of one another so that adverse chemical reactions do not occur. ... Life Technologies Corporation

07/09/15 / #20150190764

Gas spargers and related container systems

A container system includes a bag having of one or more sheets of flexible polymeric material, the bag having an interior surface at least partially bounding a chamber, the chamber being adapted to hold a fluid. A flexible sparging sheet is secured to the interior surface of the bag so that a compartment is formed between the interior surface of the bag and the sparging sheet, at least a portion of the sparging sheet allowing gas to pass therethrough. ... Life Technologies Corporation

07/02/15 / #20150185152

Illumination systems

A system for detecting emissions from a sample containing nucleotide molecules contains a sample holder, an optical illumination system, an optical sensor. The sample holder includes a plurality of spatially separated reaction sites configured to hold a sample containing nucleotide molecules. ... Life Technologies Corporation

07/02/15 / #20150184228

Compositions, methods and kits for nucleic acid synthesis and amplification

The present invention is directed to compositions, methods and kits useful for the synthesis of nucleic acid molecules. More specifically, compositions, methods and kits are provided for the amplification of nucleic acid molecules in a one-step rt-pcr procedure comprising one or more agents used to increase tolerance to pcr inhibitors.. ... Life Technologies Corporation

07/02/15 / #20150184145

Novel compounds for use in pcr systems and applications thereof

This disclosure relates to novel compounds for use in various compositions, kits and methods, including, for example, use in polymerase storage buffers and in nucleic acid synthesis or amplification reactions such as a polymerase chain reaction (pcr). Methods for preparing the novel compounds are also described.. ... Life Technologies Corporation

07/02/15 / #20150183754

Fatty acid inhibitors

Fatty acid inhibitors, pharmaceutical compositions including fatty acid inhibitors, methods for using fatty acid inhibitors to treat a variety of diseases, and methods for preparing fatty acid inhibitors are provided herein.. . ... Life Technologies Corporation

06/25/15 / #20150177211

Chemical probe compounds that become fluorescent upon reduction, and methods for their use

Chemical stain compounds containing a fluorophore and a reducible quenching unit are disclosed. The reducible quenching unit quenches the fluorophore while in its oxidized state. ... Life Technologies Corporation

06/25/15 / #20150175734

Polymer particles, nucleic acid polymer particles and methods of making and using the same

The disclosure relates to methods of making polymer particles, said methods including the steps of: making an aqueous gel reaction mixture; forming an emulsion having dispersed aqueous phase micelles of gel reaction mixture in a continuous phase; adding an initiator oil comprising at least one polymerization initiator to the continuous phase; and performing a polymerization reaction in the micelles. Further, the initiator oil is present in a volume % relative to a volume of the aqueous gel reaction mixture of between about 1 vol % to about 20 vol %. ... Life Technologies Corporation

06/25/15 / #20150175698

Recombinant antibody compositions and methods of use thereof

Provided are methods and composition useful in isolating or otherwise preparing a population of target cells. In part, it relates to recombinant antibodies comprising an exogenous proteolytic cleavage site and nucleic acid molecules encoding the antibodies.. ... Life Technologies Corporation

06/25/15 / #20150174260

Amine-containing transfection reagents and methods for making and using same

There are provided for herein novel amine-containing transfection compounds and methods for making and using same. The compounds are generally obtained by reacting a primary amine with an unsaturated compound. ... Life Technologies Corporation

06/18/15 / #20150168393

Spatial positioning of spectrally labeled beads

Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. ... Life Technologies Corporation

06/18/15 / #20150168383

Anthraquinone based near ir emitting compounds and uses thereof

Disclosed are near ir emitting fluorescent compounds; methods of making and kits containing the described compounds; and their use in fluorescence-based detection of biological materials.. . ... Life Technologies Corporation

06/18/15 / #20150168345

Self-aligned well structures for low-noise chemical sensors

In one implementation, a chemical detection device is described. The device includes a chemically-sensitive field effect transistor including a floating gate conductor coupled to a gate dielectric and having an upper surface, and a sensing material on the upper surface. ... Life Technologies Corporation

06/18/15 / #20150168344

Active chemically-sensitive sensors with in-sensor current sources

Methods and apparatus relating to fet arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.. ... Life Technologies Corporation

06/18/15 / #20150167068

Human identification using a panel of snps

The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex pcr of one or more nucleic acids which belong to a panel of single nucleotide polymorphisms (snps) useful to identify a human. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences in the panel. ... Life Technologies Corporation

06/18/15 / #20150166992

Compositions and methods for inhibition of nucleic acids function

The invention relates generally to compositions and methods for inhibiting the function of target nucleic acids by sequence specific binding. The compositions and methods can be used for inhibition of micro rnas and other relatively short non-coding rnas.. ... Life Technologies Corporation

06/11/15 / #20150161702

Methods and systems for in silico experimental design and for providing a biotechnology product to a customer

Provided herein is a method and computer program product for designing and/or simulating a biotechnology experiment in silico; and for providing and generating revenue from a customized list of one or more biotechnology products and/or services related to the in silico designed or simulated biotechnology experiment or the product of that experiment. In illustrative examples, the products and or services are indirectly related to a biomolecule designed by the in silico designed biotechnology experiment. ... Life Technologies Corporation

06/11/15 / #20150160154

Integrated sensor arrays for biological and chemical analysis

The invention is directed to apparatus and chips comprising a large scale chemical field effect transistor arrays that include an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid for analysis. In one aspect such transistor arrays have a pitch of 10 μm or less and each sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region. ... Life Technologies Corporation

06/11/15 / #20150160153

System and method for forming microwells

A method of forming a sensor component includes forming a first layer over a sensor pad of a sensor of a sensor array. The first layer includes a first inorganic material. ... Life Technologies Corporation

06/11/15 / #20150157994

Fluid mixing system with hangers

A fluid mixing system includes a support housing having an interior surface bounding a compartment, the support housing having a floor and a sidewall upstanding therefrom, an opening extending through the sidewall and communicating with the compartment. An elongated rack is disposed on the support housing adjacent to the opening. ... Life Technologies Corporation

06/04/15 / #20150152478

Gene expression profiling from ffpe samples

Methods and compositions relating to the generation and use of gene expression data from tissue samples that have been fixed and embedded are provided. The data can electronically stored and implemented as well as used to augment diagnosis and treatment of diseases.. ... Life Technologies Corporation

06/04/15 / #20150152460

Methods and compositions for seamless cloning of nucleic acid molecules

The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type iis restriction enzyme recognition sites. ... Life Technologies Corporation

06/04/15 / #20150151399

Mass finishing component enclosure device and system

An embodiment comprises a container to include a substrate during processing (e.g., centrifugal or vibratory processing) and protect the substrate from other substrates (e.g., medical implants) that are simultaneously being processed and/or from the polishing environment (e.g. Vibratory tub sidewalls) during processing. ... Life Technologies Corporation

06/04/15 / #20150151262

Systems for inactivating fluid cultures through heating

A fluid heating system includes a tank assembly having an interior surface bounding a chamber, the tank assembly having: a sidewall encircling the chamber and extending between a first end and an opposing second end, the first end bounding an opening to the chamber; and a lid movable between a first position wherein the opening to the chamber is exposed and a second position wherein the lid is disposed over the opening. A collapsible bag is removably disposed within the chamber of the tank assembly, the collapsible bag bounding a compartment adapted to hold a fluid. ... Life Technologies Corporation

05/28/15 / #20150148692

Chordal sizer

An embodiment includes a cardiovascular surgical apparatus comprising: a sleeve including a distal sleeve end, a proximal sleeve end, and a sleeve long axis extending from the distal sleeve end to the proximal sleeve end; a post including a distal post end and a proximal post end; a first projection member, coupled to the distal sleeve end, including a first projection member main body and a first projection member retention body; a second projection member coupled to the proximal sleeve end; wherein (a) the post telescopes distally from within the sleeve and away from the distal sleeve end; (b) at least one of the first and second projection members rotates at least 45 degrees with respect to another of the first and second projection members; and (c) the first projection member retention body includes a long axis generally parallel to the sleeve long axis. Other embodiments are described herein.. ... Life Technologies Corporation

05/21/15 / #20150142323

Method and system for determining an amplification quality metric

According to one exemplary embodiment, a method for providing a amplification quality metric to a user is provided. The method includes receiving amplification data from an amplification of a sample to generate an amplification curve. ... Life Technologies Corporation

05/21/15 / #20150140557

Methods, compositions, and kits for detecting allelic variants

In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as snps, or nucleotide (nt) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. ... Life Technologies Corporation

05/21/15 / #20150138913

Fluid mixing system with tiltable support housing

A fluid mixing system that can be used as a bioreactor or fermentor can include a stand and a support housing pivotably mounted to the stand. The support housing bounds a chamber into which a container assembly is removably disposed. ... Life Technologies Corporation

05/07/15 / #20150126723

Methods of synthesizing and labeling nucleic acid molecules

The present invention is generally related to composition, kits and methods for synthesizing nucleic acid molecules and particularly for synthesizing labeled nucleic acid molecules. Specifically, the invention relates to methods, kits and compositions for synthesizing indirectly labeled nucleic acid molecules. ... Life Technologies Corporation

05/07/15 / #20150126378

Methods and apparatus for measuring analytes using large scale fet arrays

Methods and apparatus relating to very large scale fet arrays for analyte measurements. Chemfet (e.g., isfet) arrays may be fabricated using conventional cmos processing techniques based on improved fet pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. ... Life Technologies Corporation

04/30/15 / #20150119291

Nucleic acid amplification

The present invention provides methods for the amplification of nucleic acid molecules. Methods for amplifying target polynucleotides, including mrna, using oligonucleotides, dna and rna polymerases are provided. ... Life Technologies Corporation

04/30/15 / #20150118753

Methods and apparatus for gas stream mass transfer with a liquid

A method and system for achieving a gas-liquid mass transfer includes delivering into a compartment of a container a liquid, the liquid having an exposed top surface disposed within the compartment. A stream of a gas is passed over the top surface of the liquid so that the stream of gas produces turbulence on the top surface that is sufficient to achieve the gas-liquid mass transfer. ... Life Technologies Corporation

04/30/15 / #20150118679

Methods and apparatus for single molecule sequencing using energy transfer detection

Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. ... Life Technologies Corporation

04/30/15 / #20150117142

Fluid mixing system with flexible drive line and foldable impeller

A fluid mixing system includes a container, such as a flexible bag, bounding a compartment. A flexible drive line is disposed within the compartment, the drive line having a first end rotatably connected to a first end of the container and an opposing second end rotatably connected to a second end of the container. ... Life Technologies Corporation

04/23/15 / #20150111214

Direct quantification of unprocessed nucleic acid samples

A workflow for direct qpcr quantification of unprocessed forensic casework samples is disclosed herein. 13 pg of dna has been detected by direct amplification from a paper substrate. ... Life Technologies Corporation

04/16/15 / #20150105275

Small rna capture, detection and quantification

Methods, compositions and kits for capturing, detecting and quantifying mature small rnas are provided herein. Embodiments of the methods comprise ligating 5′ and 3′ ligation adaptors to the 5′ and 3′ ends of the mature small rnas, respectively, in the presence of 5′ and 3′ semi-degenerate ligation splints to generate a ligation product. ... Life Technologies Corporation

04/16/15 / #20150104848

Nucleic acid-free thermostable enzymes and methods of production thereof

The present invention provides thermostable enzymes, such as dna polymerases and restriction endonucleases, that are substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in amplifying or sequencing nucleic acid molecules, including through use of the polymerase chain reaction (pcr).. ... Life Technologies Corporation

04/16/15 / #20150104823

Composition and method for measuring thallium influx and efflux

The present invention relates to methods for detecting the activity of an ion channel in a cell. The methods comprise providing a loading buffer solution to a cell that has an ion channel. ... Life Technologies Corporation

04/16/15 / #20150104801

Methods and compositions for exosome isolation

Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. ... Life Technologies Corporation

04/16/15 / #20150102234

Systems and method for fluorescence imaging

An apparatus for producing an image of blotting membranes includes an enclosure, light source, optical system, photodetector, and beamsplitter. The enclosure supports a blotting substrate comprising a first probe characterized by an excitation wavelength and an emission wavelength and a second probe characterized by an excitation wavelength and an emission wavelength. ... Life Technologies Corporation

04/09/15 / #20150100247

Methods and systems for modeling phasing effects in sequencing using termination chemistry

A method for nucleic acid sequencing includes receiving observed or measured nucleic acid sequencing data from a sequencing instrument that receives and processes a sample nucleic acid in a termination sequencing-by-synthesis process. The method also includes generating a set of candidate sequences of bases for the observed or measured nucleic acid sequencing data by determining a predicted signal for candidate sequences using a simulation framework. ... Life Technologies Corporation

04/09/15 / #20150099797

Oligonucleotide compositions with enhanced efficiency

The oligonucleotide compositions of the present invention make use of combinations of oligonucleotides. In one aspect, the invention features an oligonucleotide composition including at least 2 different oligonucleotides targeted to a target gene. ... Life Technologies Corporation

04/09/15 / #20150099644

Method of performing digital pcr

A method of detection of a target nucleic acid is provided. The method includes fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes, and subjecting the plurality of sample volumes to conditions for amplification. ... Life Technologies Corporation

04/09/15 / #20150097610

Method and system for delta double sampling

An array of sensors arranged in matched pairs of transistors with an output formed on a first transistor and a sensor formed on the second transistor of the matched pair. The matched pairs are arranged such that the second transistor in the matched pair is read through the output of the first transistor in the matched pair. ... Life Technologies Corporation

04/02/15 / #20150094212

Systems and methods for detecting structural variants

Systems and method for identifying long deletions can obtain sequencing information for a plurality of amplicons in and around a potential region from a nucleic acid sample. The sequencing information can include a plurality of reads that can be mapped to a reference sequence. ... Life Technologies Corporation

04/02/15 / #20150094211

Polymerase compositions, methods of making and using same

The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. ... Life Technologies Corporation

04/02/15 / #20150093813

Electroporator having an elongated hollow member

An electroporation apparatus comprising an elongated hollow member in order to provide a uniform electric field during electroporation, wherein specifically, electroporation is carried out by applying electric pulses through a couple of electrodes from both end parts of the elongated hollow member, after the hollow member is charged with fluid specimen including cells and material which would be injected into the cells.. . ... Life Technologies Corporation

04/02/15 / #20150093788

Methods and reagents for molecular cloning

The present invention provides compositions, methods, and kits for covalently linking nucleic acid molecules. The methods include a strand invasion step, and the compositions and kits are useful for performing such methods. ... Life Technologies Corporation

04/02/15 / #20150093787

Compositions and methods for use in recombinational cloning of nucleic acids

The present invention relates generally to compositions and methods for use in recombinational cloning of nucleic acid molecules. In particular, the invention relates to nucleic acid molecules encoding one or more recombination sites or portions thereof, to nucleic acid molecules comprising one or more of these recombination site nucleotide sequences and optionally comprising one or more additional physical or functional nucleotide sequences. ... Life Technologies Corporation

04/02/15 / #20150090936

Large stokes shift dyes

Provided herein are heptamethine cyanine dyes having a large stokes shift, and the salts and conjugates thereof. Also provided are methods of using and making such large stokes shift dyes as fluorescence resonance energy transfer (fret) acceptors or donors.. ... Life Technologies Corporation

04/02/15 / #20150090664

Sample holder with magnetic base and magnetisable body

This invention relates to a sample holder for isolating magnetically labelled particles from a non-magnetic medium in a plurality of samples. The holder comprises a magnetic base for applying a magnetic force to the magnetically labelled particles and a body which is mountable on the base and demountable therefrom. ... Life Technologies Corporation

03/26/15 / #20150087537

Methods, systems, computer readable media, and kits for sample identification

A method for sequencing a polynucleotide sample having a barcode sequence includes: introducing a series of nucleotides to the polynucleotide sample according to a predetermined order of nucleotide flows; obtaining a series of signals resulting from the introducing of nucleotides to the polynucleotide sample; and resolving the series of signals over the barcode sequence to render a flowspace string, wherein the flowspace string is a codeword of an error-correcting code that is (i) designed based on and adapted for use with the predetermined order of nucleotide flows, and (ii) capable of distinguishing any codeword in the error-correcting code from the other codewords in the error-correcting code in the presence of zero, one, and two errors.. . ... Life Technologies Corporation

03/26/15 / #20150087530

Low-volume sequencing system and method of use

Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 μl). ... Life Technologies Corporation

03/26/15 / #20150087025

Method and apparatus for automated sample manipulation

The present teachings provide apparatuses and methods for automated handling of samples, e.g., biological or chemical samples. The apparatuses and the methods of the present teachings allow automated performance of various sample manipulation steps without manual intervention. ... Life Technologies Corporation

03/26/15 / #20150083232

Vertical clamp device

A vertical clamping device is provided that supports a flow cell component in a vertical configuration in which the flow cell is on an opposite side of the vertical support from the electronic interface. The clamp includes a vertical setting to receive the flow cell component and provides an electronic interface on a vertical surface of the vertical setting. ... Life Technologies Corporation

03/19/15 / #20150080247

Systems and methods for biological analysis

A system for performing biological reactions is provided. The system includes a chip including a substrate and a plurality of reaction sites. ... Life Technologies Corporation

03/19/15 / #20150080239

Classification and actionability indices for cancer

The disclosure provides compositions, kits, and methods for detecting a plurality of genes and associated variants in a sample from a subject with cancer. The compositions, kits, and methods include a set of oligonucleotides, typically primers and/or probes that can hybridize to identify a gene variant. ... Life Technologies Corporation

03/19/15 / #20150080228

Device preparation using condensed nucleic acid particles

A method of sequencing a nucleic acid strand includes receiving particles having nucleic acid strands coupled to a polymer matrix, exposing the particles to a solution including a condensing agent, and applying the particles to a surface, the particles depositing on the surface.. . ... Life Technologies Corporation

03/19/15 / #20150079659

Serum-free mammalian cell culture medium, and uses thereof

The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. ... Life Technologies Corporation

03/19/15 / #20150079622

Modified nucleic acid binding cyanine dyes for the detection of reactive oxygen species

Disclosed herein are compounds, compositions, methods, and kits for detecting reactive oxygen species (ros) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced nucleic acid binding cyanine dyes, which dyes are probes for detecting ros and measuring oxidative stress in cells either in vitro and/or in vivo. ... Life Technologies Corporation

03/12/15 / #20150072897

Systems and methods for loading liquid samples

A sample loader for loading a liquid sample into a plurality of reaction sites within a substrate is provided. The sample loader includes a first blade, and a second blade coupled to the first blade. ... Life Technologies Corporation

03/12/15 / #20150072875

Identification of nucleic acids

This disclosure relates to methods for identifying target nucleic acids in a sample by detecting an amplified sequence corresponding to the target using a detectable probe and by monitoring its melting temperature (tm).. . ... Life Technologies Corporation

03/12/15 / #20150072396

Compounds and methods for conjugation of biomolecules

Low-copper click chemistry, 1.3-dipolar cycloadditions, and staudinger ligations for modifying biomolecules is provided. Compositions, methods, and kits relating to low-copper click chemistry, 1.3-dipolar cycloadditions, and staudinger ligations are also provided.. ... Life Technologies Corporation

03/12/15 / #20150072342

Methods of using fet labeled oligonucleotides that include a 3'-5' exonuclease resistant quencher domain and compositions for practicing the same

Methods and compositions are provided for detecting a primer extension product in a reaction mixture. In the subject methods, a primer extension reaction is conducted in the presence of a polymerase having 3′→5′ exonuclease activity and at least one fet labeled oligonucleotide probe that includes a 3′→5′ exonuclease resistant quencher domain. ... Life Technologies Corporation

03/05/15 / #20150065356

Methods of bead manipulation and forming bead arrays

According to various embodiments, a method is provided that comprises washing an array of dna-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. ... Life Technologies Corporation

03/05/15 / #20150064829

Chemical sensor with sidewall spacer sensor surface

In one implementation, a chemical sensor is described. The chemical sensor includes chemically-sensitive field effect transistor including a floating gate conductor having an upper surface. ... Life Technologies Corporation

03/05/15 / #20150064785

Cell culture media and methods

Compositions and methods are described for preparing media, feeds, and supplements. Such methods and medias may display increased stability of labile components and may use, for example, microsuspension and/or encapsulation technologies, chelation, and optionally, coating and/or mixing the labile compounds with anti-oxidants. ... Life Technologies Corporation

03/05/15 / #20150063666

Systems and methods for assessing images

Disclosed are systems and methods for assessing images in applications such as microscopic scanning of a slide having light emitting objects. In certain embodiments, such scanning can involve objects such as sequencing beads disposed on the slide to facilitate biological analysis such as nucleic acid sequencing. ... Life Technologies Corporation

03/05/15 / #20150060279

Electrophoretically enhanced detection of analytes on a solid support

The present embodiments provide systems, kits and methods suitable for performing dry or substantially dry electro-blotting analyses on immobilized protein or nucleic acid samples. Electro-blotting performed according to the presently described embodiments may include a step whereby detection of one or more immobilized proteins or nucleic acids is electrophoretically accelerated. ... Life Technologies Corporation

02/26/15 / #20150057377

Polymer-based emulsion breaking methods

A method for breaking emulsions includes applying a polymer mixture to an emulsion. The emulsion can be energized, such as through centrifugation or vibration. ... Life Technologies Corporation

02/26/15 / #20150057333

Double-stranded oligonucleotides

Antisense sequences, including duplex rnai compositions, which possess improved properties over those taught in the prior art are disclosed. The invention provides optimized antisense oligomer compositions and method for making and using the both in in vitro systems and therapeutically. ... Life Technologies Corporation

02/26/15 / #20150057182

Methods for analysis of dna fragments

A method of genotyping includes applying a sample solution including a plurality of copies of a sample polynucleotide to an array of sensors. The sample polynucleotide includes a region associated with an allele. ... Life Technologies Corporation

02/26/15 / #20150056626

Nucleic acid amplification kits for specific detection of e. coli o157:h7 without co-detection of e. coli o55:h7

Disclosed are assays, methods and kits for the specific detection of e. Coli o157:h7 and not e. ... Life Technologies Corporation

02/26/15 / #20150056621

Primers, methods and kits for detecting killer-cell immunoglobulin-like receptor alleles

Embodiments of the present invention describe primer pairs, methods and kits for identifying and/or detecting killer-cell immunoglobulin-like receptor (kir) alleles. The present primer sets include one or more primer pairs that can produce amplicons specific for an individual kir allele and that are less than 1000 bp in size. ... Life Technologies Corporation

02/26/15 / #20150053561

Particle analyzing systems and methods using acoustic radiation pressure

The present invention comprises methods and systems that use acoustic radiation pressure.. . ... Life Technologies Corporation

02/19/15 / #20150051118

Coated substrate for biological reaction systems

An apparatus for biological reactions is provided. The apparatus includes a substrate and a plurality of reaction sites within the substrate. ... Life Technologies Corporation

02/19/15 / #20150051096

Labeling and detection of post translationally modified proteins

Provided in certain embodiments are new methods for forming azido modified biomolecule conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling a biomolecules with an azide group.. ... Life Technologies Corporation

02/19/15 / #20150050685

Violet laser excitable dyes and their method of use

The present invention provides dye compounds optimally excited at about 400 nm and have a stokes shift of at least about 80 nm. These dyes find use in detection of analyte in a sample and the preparation of dye-conjugates.. ... Life Technologies Corporation

02/19/15 / #20150050660

Stable compositions for nucleic acid amplification and sequencing

The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable dna polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stablizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing. ... Life Technologies Corporation

02/12/15 / #20150045547

Synthesis of 2' , 3' -dideoxynucleosides for automated dna synthesis and pyrophosphorolysis activated polymerization

Methods for preparation of 2′,3′-dideoxynucleotides support structures, such as 2′,3′-dideoxyguanosine, 2′,3′-dideoxyadenosine, and 3′-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated dna synthesis and pyrophosphorolysis activated polymerization.. ... Life Technologies Corporation

02/12/15 / #20150045252

Systems and methods for assessing of biological samples

A system for determining the number of target nucleotide molecules in a sample includes a sample holder, an excitation optical system, an optical sensor, and an emission optical system. The sample holder is configured to receive an article comprising at least 20,000 separate reaction sites. ... Life Technologies Corporation

02/12/15 / #20150045242

Detection of listeria species in food and environmental samples, methods and compositions thereof

Embodiments of the disclosure relate to isolated nucleic acid sequences, methods of use thereof, and workflows for detection of several listeria species in a sample, particularly in a food or environmental sample. Embodiments of the disclosure may also be used to detect one or more species or strains of listeria from each other, for example l. ... Life Technologies Corporation

02/12/15 / #20150044758

Apparatus for and method of processing biological samples

The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. ... Life Technologies Corporation

02/12/15 / #20150044686

Systems and methods for containing biological samples

An article for holding a plurality of biological samples includes a substrate a substrate comprising a first surface and an opposing second surface and a plurality of reaction sites in the substrate. Each of the reaction sites extends from an opening in the first surface to an opening in the second surface. ... Life Technologies Corporation

02/12/15 / #20150044093

Systems and containers for sterilizing a fluid

A system for sterilizing a fluid includes a fluid source and a sterilization container bounding a fluid flow path that is in fluid communication with the fluid source. The sterilization container is comprised of one or more polymeric walls. ... Life Technologies Corporation

02/05/15 / #20150039383

Methods and systems for a product selection tool

. . A computer-implemented method for determining a desired product for performing a workflow is provided. The method includes receiving a selection of a desired workflow from a user. ... Life Technologies Corporation

02/05/15 / #20150038377

Microfluidic transfer pins

A liquid dispenser for a microfluidic assay system is described. The dispenser includes at least one transfer pin for transferring a microfluidic sample of liquid to a target receptacle. ... Life Technologies Corporation

02/05/15 / #20150038375

Methods and compositions for multiplex pcr

The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex pcr of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. ... Life Technologies Corporation

02/05/15 / #20150038343

Methods and compositions for multiplex pcr

The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex pcr of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. ... Life Technologies Corporation

02/05/15 / #20150038342

Methods and compositions for multiplex pcr

The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex pcr of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. ... Life Technologies Corporation

02/05/15 / #20150037792

Primers and methods for the detection and discrimination of nucleic acids

The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific pcr.. ... Life Technologies Corporation

02/05/15 / #20150037791

Methods and kits for multiplex amplification of short tandem repeat loci

Compositions, methods and kits are disclosed for use in simultaneously amplifying at least 20 specific str loci of genomic nucleic acid in a single multiplex reaction, as are methods and materials for use in the analysis of the products of such reactions. Included in the present invention are materials and methods for the simultaneous amplification of 23 and 24 specific loci in a single multiplex reaction, comprising the 13 codis loci, the amelogenin locus, an indel and at least six to ten additional str loci, including methods, kits and materials for the analysis of these loci.. ... Life Technologies Corporation

01/29/15 / #20150031579

Aza-benzazolium containing cyanine dyes

Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as dna or rna, or when associated with detergent-complexed proteins. ... Life Technologies Corporation

01/29/15 / #20150031558

Methods, compositions and systems for sample deposition

Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. ... Life Technologies Corporation

01/29/15 / #20150031551

Control nucleic acid sequences for use in sequencing-by-synthesis and methods for designing the same

A method for nucleic acid sequencing includes (a) disposing a plurality of template polynucleotide strands in a plurality of defined spaces disposed on a sensor array, at least some of the template polynucleotide strands comprising a test or control sequence; (b) exposing a plurality of the template polynucleotide strands in the defined spaces to a series of flows of nucleotide species flowed according to a predetermined ordering; and (c) determining sequence information for a plurality of the template polynucleotide strands in the defined spaces based on the flows of nucleotide species to generate a plurality of sequencing reads corresponding to the template polynucleotide strands, wherein the test or control sequence comprises a sequence determined by identifying, using a variant caller, loci with systematic errors present in a plurality of sequencing runs included in a training set of sequencing runs.. . ... Life Technologies Corporation

01/29/15 / #20150031031

Apparatus for collecting fingerprints and buccal swabs

Disclosed are devices and methods for collection, labeling and matching biological samples containing nucleic acid in conjunction with collecting at least one ridge and valley signature such as a fingerprint or footprint of an individual. Such devices and methods are used in forensic, human identification, paternity, tissue typing, and screening technologies to rapidly process an individual's identity, determine the identity of an individual along with the genotype profile of the individual.. ... Life Technologies Corporation

01/29/15 / #20150031026

Enhanced ligation reactions

In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. ... Life Technologies Corporation

01/22/15 / #20150024400

Sample collection devices, kits and methods of use

Devices, methods, and kits are disclosed for collection, labeling and analysis of samples containing a substance of interest. Such devices and methods are used in forensic, human identification, access and importation control, and other investigative technologies to collect even limited amounts of a substance of interest that may be present on a substrate and to facilitate analysis to identify the substance of interest.. ... Life Technologies Corporation

01/15/15 / #20150017703

Methods for increasing the infectivity of viruses

Methods of using viruses labeled with alkyne-modified biomolecules, such as fatty acids, carbohydrates and lipids, to treat a plant, an insect or an animal infected with a virus or to increase the infectivity of a virus, such as the human immunodeficiency virus, are provided. Also provided are methods of labeling a virus, such as human immunodeficiency virus, with an alkyne-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. ... Life Technologies Corporation

01/15/15 / #20150017688

Compositions and methods for reverse transcriptase-polymerase chain reaction (rt-pcr)

The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (rt-pcr). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step rt-pcr procedure using combinations of reverse transcriptase and thermostable dna polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. ... Life Technologies Corporation

01/08/15 / #20150011436

Coating process for microfluidic sample arrays

A differentially coated device for conducting a plurality of nano-volume specified reactions, the device comprising a platen having at least one exterior surface modified to a specified physicochemical property, a plurality of nano-volume channels, each nano-volume channel having at least one interior surface in communication with the at least one exterior surface that is selectively coated with an optionally dissolvable coating agent physisorbed to at least one interior surface, wherein the optionally dissolvable coating agent comprises a coating agent and a first component for the plurality of specified reactions. Methods for preparing and using such devices are also provided, as well as a method of registering a location of a dispenser array in relation to a microfluidic array. ... Life Technologies Corporation

01/01/15 / #20150004597

Fluorescent chemical compounds having high selectivity for double stranded dna, and methods for their use

Chemical compounds having a high selectivity for double stranded dna over rna and single stranded dna are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded dna, but exhibit reduced or no fluorescence in the absence of double stranded dna. ... Life Technologies Corporation








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