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Life Technologies Corporation patents (2016 archive)


Recent patent applications related to Life Technologies Corporation. Life Technologies Corporation is listed as an Agent/Assignee. Note: Life Technologies Corporation may have other listings under different names/spellings. We're not affiliated with Life Technologies Corporation, we're just tracking patents.

ARCHIVE: New 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 | Company Directory "L" | Life Technologies Corporation-related inventors


Cell culture media and methods

Compositions and methods are described for preparing media, feeds, and supplements. Such methods and medias may display increased stability of labile components and may use, for example, microsuspension and/or encapsulation technologies, chelation, and optionally, coating and/or mixing the labile compounds with anti-oxidants. ... Life Technologies Corporation

A graphical user interface system and method for transferring data acquisition and analysis settings

The present set of embodiments relate to a system and method for a graphical user interface for transferring data acquisition and analysis settings between different experiments. More specifically, the transferring technology disclosed herein allows for propagation of settings through a multi-tiered database structure.. ... Life Technologies Corporation

Polymer particles, nucleic acid polymer particles and methods of making and using the same

The disclosure relates to methods of making polymer particles, said methods including the steps of: making an aqueous gel reaction mixture; forming an emulsion having dispersed aqueous phase micelles of gel reaction mixture in a continuous phase; adding an initiator oil comprising at least one polymerization initiator to the continuous phase; and performing a polymerization reaction in the micelles. Further, the initiator oil is present in a volume % relative to a volume of the aqueous gel reaction mixture of between about 1 vol % to about 20 vol %. ... Life Technologies Corporation

Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging

In some embodiments, the disclosure relates generally to methods, as well as related systems, compositions, kits, apparatuses and computer-readable media, comprising a multiplex molecular tagging procedure that employs a plurality of tags that are appended to a plurality of polynucleotides. The tags have characteristics, including a sequence, length and/or detectable moiety, or any other characteristic, that uniquely identifies the polynucleotide molecule to which it is appended, and permits tracking individual tagged molecules in a mixture of tagged molecules. ... Life Technologies Corporation

Regulated vacuum off-gassing of gas filter for fluid processing system and related methods

A method for filtering a gas includes delivering a gas into a compartment of a gas filter, the compartment being at least partially bounded by a casing comprised of a flexible sheet of polymeric film. A partial vacuum is applied to the gas filter so that the partial vacuum assists in drawing the gas through a porous filter body of the gas filter that is at least partially disposed within the compartment of the gas filter.. ... Life Technologies Corporation

Fluorogenic hydrazine-substituted compounds

The present disclosure is directed to fluorogenic schiff base-forming dyes capable of detecting analytes containing aldehyde and ketone groups. The dyes contain nucleophilic hydrazinyl appendages and are capable of binding and detecting analytes in situ.. ... Life Technologies Corporation

Modified carbocyanine dyes and their conjugates

Chemically reactive carbocyanine dyes incorporating an indolium ring moiety that is substituted at the 3-position by a reactive group or by a conjugated substance, and their uses, are described. Conjugation through this position results in spectral properties that are uniformly superior to those of conjugates of spectrally similar dyes wherein attachment is at a different position. ... Life Technologies Corporation

Violet laser excitable dyes and their method of use

The present invention provides dye compounds optimally excited at about 400 nm and have a stokes shift of at least about 80 nm. These dyes find use in detection of analyte in a sample and the preparation of dye-conjugates.. ... Life Technologies Corporation

Ultra-fast nucleic acid sequencing device and a method for making and using the same

A system and method employing at least one semiconductor device, or an arrangement of insulating and metal layers, having at least one detecting region which can include, for example, a recess or opening therein, for detecting a charge representative of a component of a polymer, such as a nucleic acid strand proximate to the detecting region, and a method for manufacturing such a semiconductor device. The system and method can thus be used for sequencing individual nucleotides or bases of ribonucleic acid (rna) or deoxyribonucleic acid (dna). ... Life Technologies Corporation

Lysis buffers for extracting nucleic acids

The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. ... Life Technologies Corporation

Methods for the analysis of high resolution melt curve data

The present application provides for various embodiments of methods for the analysis of high resolution melt (hrm) curve data; where statistical assay variations in melt curve data may result from system noise in an analysis system. Such system noise may arise from various sources, such as the thermal non-uniformity of a thermocycler block in a thermal cycler apparatus, a detection system, etc. ... Life Technologies Corporation

Rapid protein labeling and analysis

The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. ... Life Technologies Corporation

Sample preparation devices and methods

A device for sample processing can include at least one chamber having an egress, said chamber being configured to receive a sample for processing, a filter through which at least some sample portions in the at least one chamber flow, and a barrier member disposed in a first state to contain sample in the at least one chamber. Upon sufficient conditions, the barrier member can be alterable to a second state to permit flow of at least some sample portions contained in the chamber in a flow direction toward the egress and through the filter.. ... Life Technologies Corporation

Use of novel coumarins as glutathione and thiol labels

Fluorescent quinolizinocoumarin compounds substituted with electrophilic reactive groups that bind thiol compounds are described. The compounds are useful in detecting oxidative stress and processes associated therewith in live cells.. ... Life Technologies Corporation

11/17/16 / #20160333402

Barcode sequences, and related systems and methods

Methods, system, and kits are provided for sample identification, and, more specifically, for designing, and/or making, and/or using sample discriminating codes or barcodes for identifying sample nucleic acids or other biomolecules or polymers. For example, a plurality of flowspace codewords may be generated, the codewords comprising a string of characters. ... Life Technologies Corporation

11/17/16 / #20160333401

Computational methods for translating a sequence of multi-base color calls to a sequence of bases

Disclosed are systems and methods for resequencing using color calls. A dna sample is encoded and sequenced according to a multi-base code producing a string of read color calls for a fragment of the sample. ... Life Technologies Corporation

11/17/16 / #20160332133

Apparatus for assay, synthesis and storage, and methods of manufacture, use, and manipulation thereof

The invention features methods of making devices, or “platens”, having a high-density array of through-holes, as well as methods of cleaning and refurbishing the surfaces of the platens. The invention further features methods of making high-density arrays of chemical, biochemical, and biological compounds, having many advantages over conventional, lower-density arrays. ... Life Technologies Corporation

11/10/16 / #20160326580

Methods and apparatus for measuring analytes using large scale fet arrays

Methods and apparatus relating to very large scale fet arrays for analyte measurements. Chemfet (e.g., isfet) arrays may be fabricated using conventional cmos processing techniques based on improved fet pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. ... Life Technologies Corporation

11/10/16 / #20160326515

Methods and kits for extraction of dna

Methods and materials are disclosed for use in recovering a biopolymer from a solution. In particular, the invention provides methods for extraction and isolation of nucleic acids from biological materials. ... Life Technologies Corporation

11/10/16 / #20160326291

Hydrophobic diacrylamide compound

A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.. ... Life Technologies Corporation

11/03/16 / #20160321398

Systems and methods for the analysis of protein melt curve data

The present teachings relate to embodiments of systems and methods for the analysis of melt curve data for a plurality of samples. According to various embodiments, a melting temperature (tm) may be determined across a range of different types of protein melt curve data, having variability over a plurality of analytical attributes in order to accommodate the complexity of protein melt curve data. ... Life Technologies Corporation

11/03/16 / #20160320273

Methods and compositions for exosome isolation

Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. ... Life Technologies Corporation

10/27/16 / #20160313280

Methods and systems for variant detection

In one exemplary embodiment, a method for detecting variants in electropherogram data is provided. The method includes receiving electropherogram data from an instrument and analyzing the electropherogram data to identify mixed bases in the electropherogram data. ... Life Technologies Corporation

10/27/16 / #20160313224

Method for preparing ffpe quality control material with bulking agent

This specification relates to formalin-fixed embedded quality control material containing a bulking agent for use for validation, verification, and to nm controls for molecular assays. The quality control material can be used for a variety of tissues and for a variety of molecular assays. ... Life Technologies Corporation

10/27/16 / #20160312274

Methods and systems for volume variation modeling in digital pcr

A method for performing digital polymerase chain reaction (dpcr) is provided. The method includes partitioning a biological sample volume including a plurality of target nucleic acids into a plurality of partitions, where at least one partition includes at least one target nucleic acid. ... Life Technologies Corporation

10/27/16 / #20160312236

Molecular biology tools for algal engineering

The present invention provides compositions and methods for the genetic manipulation of algal cells. The compositions and methods allow enhanced transfer of genetic material into algal cells and the cloning and selection of genetically modified cells. ... Life Technologies Corporation

10/20/16 / #20160305964

Fluorescent metal ion indicators with large stokes shifts

. . The present invention provides fluorogenic compounds for the detection of target metal ions wherein the compounds exhibit a stokes shift greater than 50 nm and the detectable signal is modulated by photoinduced electron transfer (pet). The present compounds consist of three functional elements, the ion sensing moiety (chelating moiety), the reporter moiety (fluorophore or fluorescent protein) and spacer or linker between the sensing and reporter moieties of the present compound that allows for pet upon binding of a metal ion and excitation by an appropriate wavelength.. ... Life Technologies Corporation

10/20/16 / #20160305905

Chemical coating of microwell for electrochemical detection device

The described embodiments may provide a method of fabricating a chemical detection device. The method may comprise forming a microwell above a cmos device. ... Life Technologies Corporation

10/20/16 / #20160305881

Optical instrument including excitation source

An optical instrument is provided for simultaneously illuminating two or more spaced-apart reaction regions with an excitation beam generated by a light source. A collimating lens can be disposed along a beam path between the light source and the reaction regions to form bundles of collimated excitation beams, wherein each bundle corresponds to a respective reaction region. ... Life Technologies Corporation

10/20/16 / #20160304844

Mutant rb69 dna polymerase

Provided herein are mutant dna-dependent polymerases which are derived from, or otherwise related to, wild type rb69 dna polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. ... Life Technologies Corporation

10/20/16 / #20160304829

Automated superparamagnetic particle handling system

Methods of reversal of the binding between a biotin compound and a biotin-binding compound are disclosed. A method of reversibly releasing a biotinylated moiety from a streptavidin (or avidin) coated support is shown as an example. ... Life Technologies Corporation

10/20/16 / #20160304825

Filter systems for separating microcarriers from cell culture solutions

A filter assembly for separating microcarriers from a fluid medium includes a collapsible container bounding a sterile compartment adapted to hold a fluid. An inlet port is attached to the container through which fluid flows into the compartment. ... Life Technologies Corporation

10/13/16 / #20160299145

Methods and kits for detecting prostate cancer biomarkers

Provided herein are novel autoantibody biomarkers, and panels for detecting autoantibody biomarkers for prostate cancer, and methods and kits for detecting these biomarkers in the serum of individuals suspected of having prostate cancer.. . ... Life Technologies Corporation

10/13/16 / #20160298071

Methods and apparatus for gas stream mass transfer with a liquid

A system for performing a gas-liquid mass transfer includes a container bounding a compartment and having a top wall, a bottom wall, and an encircling sidewall extending therebetween. A first opening is formed on the container so as to communicate with the compartment. ... Life Technologies Corporation

10/06/16 / #20160290994

Substrates and methods for staining live stem cells

The invention relates to novel substrates and methods for staining live stem cells. The stain may be used to identify induced pluripotent stem cell colonies during the process of somatic cell reprogramming.. ... Life Technologies Corporation

09/29/16 / #20160281095

Alkynyl-derivatized cap analogs, preparation and uses thereof

Alkynyl-derivatized cap analogs, alkynyl-modified capped rna, 1,4-disubstituted triazole-derivatized capped rna, methods of preparation, methods of isolation, and uses thereof are provided. The “click” modification facilitates detection and isolation of capped rnas and the 1,4-disubstituted triazole derivatives formed by the “click” reaction are useful for producing rna transcripts and encoded protein.. ... Life Technologies Corporation

09/29/16 / #20160279677

Method for treating a semiconductor device

A method of treating a sensor array including a plurality of sensors and an isolation structure, where a sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array and the isolation structure is disposed between the sensor pad and sensor pads of other sensors of the plurality of sensors, comprises exposing the sensor pad and the isolation structure to a non-aqueous organo-silicon solution including an organo-silicon compound and a first non-aqueous carrier; applying an acid solution including an organic acid and a second non-aqueous carrier to the sensor pad; and rinsing the acid solution from the sensor pad and the isolation structure.. . ... Life Technologies Corporation

09/22/16 / #20160275687

Methods and systems for analyzing biological reaction systems

A method for analyzing biological reaction systems is provided. The method includes receiving an image of a substrate including a plurality of reaction sites after a biological reaction has taken place. ... Life Technologies Corporation

09/22/16 / #20160275149

Methods and systems for visualizing data quality

A method for generating a data visualization is provided. The method includes receiving a plurality of data points related to fluorescent emissions values from a plurality of reaction sites. ... Life Technologies Corporation

09/22/16 / #20160274056

Methods and apparatus for measuring analytes using large scale fet arrays

Methods and apparatus relating to very large scale fet arrays for analyte measurements. Chemfet (e.g., isfet) arrays may be fabricated using conventional cmos processing techniques based on improved fet pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. ... Life Technologies Corporation

09/22/16 / #20160272955

Mutant dna polymerase blends and mutant dna polymerases

A thermostable dna polymerase composition comprising at least two dna polymerases, one of which is substantially reduced in 5′-exonuclease activity and one of which has 5′-exonuclease activity. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, dna sequencing, nucleic acid amplification and cdna synthesis.. ... Life Technologies Corporation

09/22/16 / #20160272954

Nucleic acid amplification

In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. ... Life Technologies Corporation

09/22/16 / #20160271604

Systems and methods for loading liquid samples

A sample loader for loading a liquid sample into a plurality of reaction sites within a substrate is provided. The sample loader includes a first blade, and a second blade coupled to the first blade. ... Life Technologies Corporation

09/22/16 / #20160270915

Heart valve sewing cuff

An embodiment of the invention includes a sewing cuff for aortic heart valves that better approximates native anatomy by better mating with the crown-like anatomical annulus. Limiting distortion of the crown-like annulus provides better blood flow and overall valve function and provides a physician greater ease of implantation since native anatomy is not flattened. ... Life Technologies Corporation

09/15/16 / #20160265065

Classification and actionability indices for lung cancer

The disclosure provides compositions, kits, and methods for detecting a plurality of genes and associated variants in a sample from a subject with lung cancer. The compositions, kits, and methods include a set of oligonucleotides, typically primers and/or probes that can hybridize to identify a gene variant. ... Life Technologies Corporation

09/15/16 / #20160265045

Polymerase compositions and methods

Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. ... Life Technologies Corporation

09/15/16 / #20160265044

Conjugates of biomolecules to nanoparticles

Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. ... Life Technologies Corporation

09/15/16 / #20160265031

Methods, compositions and kits for small rna capture, detection and quantification

Methods, compositions and kits for capturing, detecting and quantifying mature small rnas are provided herein. Embodiments of the methods comprise tailing both the 5′ and 3′ ends of mature small rna by ligating a 5′ ligation adaptor to the 5′ end and polyadenylating the 3′ end. ... Life Technologies Corporation

09/15/16 / #20160264950

Reversibly inactivated thermostable reverse transcriptases, compositions and methods for use

The present invention provides reversibly inactivated reverse transcriptase enzymes, particularly those having increased thermostability and/or thermoreactivity and compositions, methods and kits that include such enzymes, for the reverse transcription of nucleic acid molecules. Also provided are compositions and methods for the reactivation of reversibly inactivated reverse transcriptases. ... Life Technologies Corporation

09/15/16 / #20160263578

Pinch flow regulator

A valve for regulating fluid flow includes a housing base defining a lower cavity and comprising a pinch structure within the lower cavity, a gas inlet providing external access to the lower cavity, a base fluid inlet, and a base fluid outlet. A housing cover defines an upper cavity and comprises a cover fluid inlet and a cover fluid outlet. ... Life Technologies Corporation

09/08/16 / #20160258056

Fluidized bed pyrocarbon coating

. . Arrangements are provided for assembling multiple substrates for coating within a fluidized bed coater so as to deposit a coating of uniform thickness across the entire exterior surface thereof. One embodiment includes a method for coating orthopedic implants having convex and concave surfaces with pyrocarbon by pyrolytic decomposition of a hydrocarbon.. ... Life Technologies Corporation

09/08/16 / #20160258011

Surface stabilization of biosensors

A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. ... Life Technologies Corporation

09/01/16 / #20160251709

Direct quantification of unprocessed nucleic acid samples

A workflow for direct qpcr quantification of unprocessed forensic casework samples is disclosed herein. 13pg of dna has been detected by direct amplification from a paper substrate. ... Life Technologies Corporation

09/01/16 / #20160251707

Karyotyping assay

This disclosure relates to methods and kits for karyotyping in which chromosomes are interrogated by amplifying loci that are not within copy number variable regions thereof.. . ... Life Technologies Corporation

09/01/16 / #20160251664

Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites

The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. ... Life Technologies Corporation

08/25/16 / #20160244822

Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis

Nucleotide triphosphate probes containing a molecular and/or atomic tag on a γ and/or β phosphate group and/or a base moiety having a detectable property are disclosed, and kits and method for using the tagged nucleotides in sequencing reactions and various assay. Also, phosphate and polyphosphate molecular fidelity altering agents are disclosed.. ... Life Technologies Corporation

08/18/16 / #20160239990

Methods and systems for experiment design and analysis

In one exemplary embodiment, a method for identifying a reaction site associated with an amplification curve from a plurality of amplification curves is provided. The method includes receiving amplification data from a plurality of reaction sites, wherein each reaction site contains a sample. ... Life Technologies Corporation

08/18/16 / #20160239605

Computer implemented method for indexing reference genome

A method for indexing a reference genome is provided. The method includes selecting a reference genome to index, calculating a first minimum index region size, assigning a first position number to a first index region of the reference genome, assigning a second position number to a second index region of the reference genome, and storing the association of the first and second position numbers to index regions in a hash table. ... Life Technologies Corporation

08/18/16 / #20160237474

Methods and systems for pure dye instrument nomalization

The present teachings relate to a method and system for normalizing spectra across multiple instruments. In an embodiment of the present invention, the method comprises at least one reference instrument and a test instrument. ... Life Technologies Corporation

08/11/16 / #20160231250

Methods and systems for determining optical regions of interest

The present teachings relate to a method and system for determining regions of interest (roi) for one or more biological samples in a laboratory instrument. The method can include an optical system capable of imaging florescence emission from a plurality of sample wells. ... Life Technologies Corporation

08/11/16 / #20160231246

Methods and systems for biological instrument calibration

In one exemplary embodiment, a method for calibrating an instrument is provided. The instrument includes an optical system capable of imaging florescence emission from a plurality of reaction sites. ... Life Technologies Corporation

08/11/16 / #20160231245

Methods and systems for instrument validation

In one exemplary embodiment, a method for validating an instrument is provided. The method includes receiving amplification data from a validation plate to generate a plurality of amplification curves. ... Life Technologies Corporation

08/11/16 / #20160230214

Centrifuge and method for loading a device

An apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. In addition, the apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles and an apparatus for loading such particles onto a sensor array. ... Life Technologies Corporation

08/11/16 / #20160230210

Systems and methods for assessing biological samples

An instrument for biological analysis includes a base, an excitation source, an optical sensor, an excitation optical system, and an emission optical system. The base is configured to receive a sample holder comprising a plurality of biological samples. ... Life Technologies Corporation

08/11/16 / #20160230209

System and methods for calibrating binding dyes

Some embodiments describe a computer-implemented method for calibrating a fluorescent dye. The method can comprise imaging a sample holder, loaded into an instrument, at more than one channel. ... Life Technologies Corporation

08/11/16 / #20160230156

Recombinant fusion protein possessing nuclease and phosphatase activity

Disclosed herein is a fusion protein possessing both nuclease and phosphatase activities. The described fusion protein simplifies the processing of amplified dna to degrade residual primers and nucleotide triphosphates and thereby facilitates subsequent dna analysis.. ... Life Technologies Corporation

08/11/16 / #20160228877

Vertical clamp device

A vertical clamping device is provided that supports a flow cell component in a vertical configuration in which the flow cell is on an opposite side of the vertical support from the electronic interface. The clamp includes a vertical setting to receive the flow cell component and provides an electronic interface on a vertical surface of the vertical setting. ... Life Technologies Corporation

08/11/16 / #20160228876

Systems and methods for biological analysis

A biological analysis system is provided. The system comprises a sample block assembly. ... Life Technologies Corporation

08/11/16 / #20160228875

Thermal cycler cover

In one aspect, a thermal cycler system is disclosed. The thermal cycler can be comprised of a device housing and a cover that is operably connected to the device housing. ... Life Technologies Corporation

08/04/16 / #20160222379

Use of multiple recombination sites with unique specificity in combinational cloning

The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites with unique specificity. ... Life Technologies Corporation

08/04/16 / #20160220372

Rapid deployment artificial chordae tendinae system

An embodiment includes an apparatus comprising: a hollow needle with a narrowed inner diameter in a distal third of the needle; and a flexible chord coupled to proximal and distal conduits; wherein the distal conduit is in the needle and distal to the narrowed inner diameter and the proximal conduit is in the needle and proximal to the narrowed inner diameter; wherein: (a)(i) long axes of the proximal and distal conduits are substantially parallel to a long axis of the needle when the proximal and distal conduits are in the needle, and (a)(ii) the proximal and distal conduits are configured to rotate when deployed from the needle such that their long axes are not parallel to a long axis of the chord when the chord is fully extended. Other embodiments are described herein.. ... Life Technologies Corporation

07/28/16 / #20160215281

Separation and purification of nucleic acid from paraffin-containing samples

Disclosed are rapid and reproducible methods for separating and purifying nucleic acids from paraffin-containing tissue samples. The disclosed methods involve a melting step, where paraffin-containing tissue samples are heated in the presence of a detergent, causing the paraffin to melt and tissue sample cells to lyse. ... Life Technologies Corporation

07/28/16 / #20160214110

Apparatuses, systems and methods for providing thermocycler thermal uniformity

A thermal block assembly including a sample block and two or more thermoelectric devices, is disclosed. The sample block has a top surface configured to receive a plurality of reaction vessels and an opposing bottom surface. ... Life Technologies Corporation

07/21/16 / #20160208342

System and method for determining copies-per-unit-volume using pcr and flow control of droplets

Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. ... Life Technologies Corporation

07/21/16 / #20160208318

Nucleotide transient binding for sequencing methods

Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (snp) analyses. ... Life Technologies Corporation

07/21/16 / #20160207045

Labels, containers, system and methods for providing reagents

The invention relates to systems and methods for marketing and using products such as liquid materials, especially liquid reagents for use in microbiological and cellular biological laboratory settings include the use of unique color and simple numeric or alphanumeric identifiers to quickly and easily identify any product from a catalog list of products. Methods of marketing, advertising and producing such products are also disclosed. ... Life Technologies Corporation

07/14/16 / #20160202209

Electric field directed loading of microwell array

An apparatus includes a device substrate including an array of sensors. Each sensor of the array of sensors can include a electrode structure disposed at a surface of the device substrate. ... Life Technologies Corporation

07/07/16 / #20160194707

3' biased detection of nucleic acids

The invention provides materials and methods for the detection of nucleic acid expression via the 3′ portion of expressed sequences. Embodiments of the invention include the use of microarrays comprising nucleic acid probes that are complementary to the 3′ and of expressed sequences and by the use of quantitative pcr (q-pcr) based amplification of sequences found at or near the 3′ end of expressed sequences. ... Life Technologies Corporation

07/07/16 / #20160194694

Multiplex transcriptome analysis

In some embodiments, the disclosure relates generally to methods, compositions, systems, apparatuses and kits comprising a multiplex nucleic acid amplification reaction that employs a plurality (e.g., hundreds, thousands, tens-of-thousands or hundreds-of-thousands) of different target-specific primer pairs that enable substantially simultaneous amplification of a plurality of different target sequences-of-interest in a single reaction mixture. In some embodiments, the multiplex nucleic acid amplification reaction generates a plurality of amplicons having sequences derived from a sample containing rna or dna, including whole transcriptome or genomic samples. ... Life Technologies Corporation

07/07/16 / #20160194629

Scaffolded nucleic acid polymer particles and methods of making and using

The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. ... Life Technologies Corporation

07/07/16 / #20160194595

Systems for inactivating fluid cultures through heating

A fluid heating system includes a tank assembly having an interior surface bounding a chamber, the tank assembly including a sidewall encircling the chamber and extending between a first end and an opposing second end, the first end bounding an opening to the chamber, and a lid movable between a first position wherein the opening to the chamber is exposed and a second position wherein the lid is disposed over the opening. A collapsible bag is removably disposed within the chamber of the tank assembly, the collapsible bag bounding a compartment adapted to hold a fluid. ... Life Technologies Corporation

07/07/16 / #20160193576

Fluid mixing system with steady support

A fluid mixing system includes a flexible bag having a first end, an opposing second end, and an interior surface bounding a compartment. A first rotational assembly includes a first casing mounted to the first end of the flexible bag and a first hub rotatably mounted to the first casing. ... Life Technologies Corporation

06/16/16 / #20160173080

System for reducing noise in a chemical sensor array

A system including a power supply and a clock circuitry to generate a plurality of clock signals. Each clock signal is synchronous with a primary clock signal. ... Life Technologies Corporation

06/16/16 / #20160168635

Integrated sensor arrays for biological and chemical analysis

The invention is directed to apparatus and chips comprising a large scale chemical field effect transistor arrays that include an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid for analysis. In one aspect such transistor arrays have a pitch of 10 μm or less and each sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region. ... Life Technologies Corporation

06/16/16 / #20160168634

Windowed sequencing

In one implementation, a method is described. The method includes determining an operational characteristic of sensors of a sensor array. ... Life Technologies Corporation

06/16/16 / #20160168626

Systems and methods for biological analysis

A case for containing a plurality of biological samples contains a base, a substrate and a cover. The substrate is attached to the base and comprises a plurality of reaction regions configured to receive one or more biological samples. ... Life Technologies Corporation

06/16/16 / #20160168537

Differentiated cellular compositions and methods for their preparation and use

The present invention relates to methods for culturing progenitor cells to differentiate primarily into a single population of cells with the same phenotype, and to compositions thereof. In particular, it relates to methods for culturing liver progenitor cells to differentiate primarily into a single population of hepatocyte-like cells, and to compositions thereof.. ... Life Technologies Corporation

06/16/16 / #20160167044

Nanoliter array loading

An interface is provided for storing microfluidic samples in a nanoliter sample chip. A fluid access structure provides a fluid access region to a selected subset of sample wells from an array of sample wells. ... Life Technologies Corporation

06/09/16 / #20160161496

Cells having a spectral signature and methods of preparation and use thereof

Methods, compositions and articles of manufacture for encoding a cell with semiconductor nanocrystals and/or other fluorophors are provided. The encoded cells can be subjected to functional assays in mixed populations, and an assay result can be determined and associated with individual cells by virtue of their code. ... Life Technologies Corporation

06/09/16 / #20160161398

Spatial positioning of spectrally labeled beads

Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. ... Life Technologies Corporation

06/09/16 / #20160160265

Devices and methods for thermally-mediated chemical reactions

One aspect of the invention provides container for thermal cycling a plurality of samples in a microfluidic array. The container includes a plurality of walls defining an interior volume and a conductive member for heating the interior volume. ... Life Technologies Corporation

06/09/16 / #20160158711

Gas spargers and related container systems

A container assembly includes a container at least partially bounding a chamber and having a wall with an opening extending therethrough. A tubular stem bounds a passageway and outwardly projects from the wall of the container so that the passageway communicates with the opening. ... Life Technologies Corporation

06/09/16 / #20160158394

Systems and containers for sterilzing a fluid

A system for sterilizing a fluid includes a sterilization container bounding a fluid flow path that can be coupled with a fluid source. The sterilization container is comprised of one or more polymeric walls. ... Life Technologies Corporation

06/02/16 / #20160154990

Simultaneous acquisition of biometric data and nucleic acid

Disclosed are devices and methods for collection and analyzing biological samples containing nucleic acid in conjunction with collecting at least one ridge and valley signature of a test subject, while keeping the sample and signature associated with each other. Such devices and methods are used in forensic, human identification, screening, and access control technologies to rapidly process an individual's identity or determine the identity of an individual.. ... Life Technologies Corporation

06/02/16 / #20160153930

Chemical device with thin conductive element

In one implementation, a chemical device is described. The sensor includes a chemically-sensitive field effect transistor including a floating gate structure having a plurality of floating gate conductors electrically coupled to one another. ... Life Technologies Corporation

06/02/16 / #20160153035

Workflow for detection of ligands using nucleic acids

This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.. . ... Life Technologies Corporation

06/02/16 / #20160152939

Heat exchanger system with flexible bag

A heat exchanger system includes a heat exchanger having a body with a first side face and an opposing second side face, the body bounding a fluid channel disposed between the opposing side faces and extending between an inlet port and an outlet port. A flexible first bag has of one or more sheet of polymeric material and bounds a fluid pathway that extends between a fluid inlet and a fluid outlet. ... Life Technologies Corporation

05/19/16 / #20160140291

Systems and methods for sequence data alignment quality assessment

. . A computer-implemented method for classifying alignments of paired nucleic acid sequence reads is disclosed. A plurality of paired nucleic acid sequence reads is received, wherein each read is comprised of a first tag and a second tag separated by an insert region. ... Life Technologies Corporation

05/19/16 / #20160139135

Optically-detectable enzyme substrates and their method of use

The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. ... Life Technologies Corporation

05/19/16 / #20160138100

Method of determining the nucleotide sequence of oligonucleotides and dna molecules

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of dna polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a dna fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. ... Life Technologies Corporation

05/19/16 / #20160137762

Hydrophilic polymeric particles and methods for making and using same

A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.. ... Life Technologies Corporation

05/12/16 / #20160130651

Biological analysis systems, devices, and methods

A device for performing biological sample reactions may include a plurality of flow cells configured to be mounted to a common microscope translation stage, wherein each flow cell is configured to receive at least one sample holder containing biological sample. Each flow cell also may be configured to be selectively placed in an open position for positioning the at least one sample holder into the flow cell and a closed position for reacting biological sample contained in the at least one sample holder. ... Life Technologies Corporation

05/12/16 / #20160130644

Thiolated nucleotide analogues for nucleic acid synthesis

The present disclosure provide systems, compositions, methods, reagents, kits and products for extending a nucleic acid that includes incorporating a nucleotide residue at a terminus of a nucleic acid using a polymerase enzyme and at least one nucleotide, wherein the at least one nucleotide includes a thiophosphate moiety, and wherein the at least one nucleotide is resistant to hydrolysis by phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of a phosphatase. ... Life Technologies Corporation

05/12/16 / #20160130637

Dual labeling methods for measuring cellular proliferation

The present invention provides a method for measuring cellular nascent nucleic acid synthesis by dual pulse labeling of nucleic acid. The first pulse labeling of nucleic acid with a nucleoside analog allows establishment of a baseline nucleic acid synthesis rate. ... Life Technologies Corporation

04/28/16 / #20160115524

Nucleic acid preparation method

A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support whilst maintaining it on the support. ... Life Technologies Corporation

04/07/16 / #20160097736

Ion-sensing charge-accumulation circuits and methods

An ion-sensitive circuit can include a charge accumulation device, to accumulate a plurality of charge packets as a function of an ion concentration of a fluid, and at least one control and readout transistor, to generate an output signal as a function of the accumulated plurality of charge packets, the output signal representing the ion concentration of the solution. The charge accumulation device can include a first charge control electrode above a first electrode semiconductor region, an electrically floating gate structure above a gate semiconductor region and below an ion-sensitive passivation surface, a second charge control electrode above a second electrode semiconductor region, and a drain diffusion region. ... Life Technologies Corporation

04/07/16 / #20160097095

Hydrophilic polymeric particles and methods for making and using same

A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.. ... Life Technologies Corporation

04/07/16 / #20160097094

Hydrophilic polymeric particles and methods for making and using same

A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.. ... Life Technologies Corporation

04/07/16 / #20160097091

Sequencing methods, compositions and systems

In some embodiments, the disclosure relates generally to methods, as well as compositions, systems, kits and apparatuses, for performing nucleotide incorporation, comprising: (a) providing a surface including one or more reaction sites containing a polymerase and a nucleic acid template that has, or is hybridized to, an extendible end; (b) performing a first nucleotide flow by contacting one or more of the reaction sites with a first solution including one or more types of terminator nucleotide; (c) incorporating at least one type of terminator nucleotide at the extendible end of the nucleic acid template contained within at least one of the reaction sites using the polymerase; and (d) detecting a non-optical signal indicating the nucleotide incorporation using a sensor that is attached or operatively linked to the at least one reaction site.. . ... Life Technologies Corporation

04/07/16 / #20160096175

Systems and methods for biological analysis

A system for processing a plurality of biological samples contains a support and a temperature controller. The support is configured to hold a case that includes an inner chamber and a substrate located within the inner chamber, the substrate containing a plurality of isolated reaction sites containing one or more biological samples. ... Life Technologies Corporation

04/07/16 / #20160096131

Regulated vacuum off-gassing of gas filter for fluid processing system and related methods

A method for filtering a gas includes sparging a gas through a liquid within a compartment of a container. In one embodiment the container can comprise a flexible bag. ... Life Technologies Corporation

04/07/16 / #20160095279

Methods and systems for culturing microbial and cellular seed cultures

A method for culturing a microbial or cellular seed culture includes positioning a first flexible bag within a chamber of a first retainer, the first retainer being secured to a mixer table. A culture is produced within a compartment of the flexible bag, the culture comprising a growth media and a microbial or cellular starter culture added thereto. ... Life Technologies Corporation

03/31/16 / #20160089649

Multi-through hole testing plate for high throughput screening

A method for holding samples for analysis and an apparatus thereof includes a testing plate with a pair of opposing surfaces and a plurality of holes. Each of the holes extends from one of the opposing surfaces to the other one of the opposing surfaces. ... Life Technologies Corporation

03/17/16 / #20160078094

Systems and methods for annotating biomolecule data

Systems, methods, software and computer-usable media for annotating biomolecule-related data are disclosed. In certain exemplified embodiments, the biomolecules can be nucleic acids and the data can be sequence-related data. ... Life Technologies Corporation

03/17/16 / #20160077727

Online protocol community

Provided herein is a computer-implemented method of personalizing a protocol in an online community of a plurality of users. The computer-implemented method includes providing a first protocol selected by a first user in the online community and receiving a request from the first user to modify the first protocol. ... Life Technologies Corporation

03/17/16 / #20160077045

Chemical sensor with consistent sensor surface areas

A chemical sensor is described. The chemical sensor includes a chemically-sensitive field effect transistor including a floating gate conductor having an upper surface. ... Life Technologies Corporation

03/17/16 / #20160076096

Methods and systems for nucleic acid sequencing validation, calibration and normalization

A system for performing quality control for nucleic acid sample sequencing is disclosed. The system comprises a set of solid supports, each solid support having attached thereto a plurality of nucleic acid sequences, wherein the set comprises plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto. ... Life Technologies Corporation

03/17/16 / #20160076090

Methods, compositions, and kits for detecting allelic variants

A system for performing quality control for nucleic acid sample sequencing is disclosed. The system comprises a set of solid supports, each solid support having attached thereto a plurality of nucleic acid sequences, wherein the set comprises plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto. In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as snps, or nucleotide (nt) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. ... Life Technologies Corporation

03/17/16 / #20160075800

Process for the preparation of monodisperse polymer particles

A process for the preparation of monodisperse polymer particles which are formed by contacting monomers with aqueous dispersions comprising monodisperse swellable seed polymers/oligomers, and initiating polymerization in the presence of a steric stabilizer. The resulting swollen seed particles are characterized by the particle mode diameter.. ... Life Technologies Corporation

03/03/16 / #20160063584

E-commerce systems and methods

The invention is in general directed to methods and processes for generating revenue via an electronic commerce website. The invention is also directed to methods of processing product orders and selling product related to protocols, workflows, or other applications. ... Life Technologies Corporation

03/03/16 / #20160061774

Gel electrophoresis, imaging, and analysis methods, devices, systems, and materials

The present teachings provide methods, devices, systems, and materials for performing electrophoresis in an automated fashion. The electrophoresis system may simultaneously image gel during an electrophoresis run. ... Life Technologies Corporation

03/03/16 / #20160061773

Method for treating a semiconductor device

A sensor array includes a plurality of sensors. A sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array. ... Life Technologies Corporation

03/03/16 / #20160061772

Methods and apparatus for measuring analytes

Methods and apparatus relating to fet arrays including large fet arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.. ... Life Technologies Corporation

02/25/16 / #20160054327

Rapid protein labeling and analysis

The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. ... Life Technologies Corporation

02/25/16 / #20160054303

Compositions and assays for determining cell viability

Provided are compositions and methods useful in evaluation of cell health and metabolism, cell viability, proliferation, and the effects of compounds on these qualities. The assays provided are rapid, robust, nontoxic and suitable for use with high throughput devices.. ... Life Technologies Corporation

02/25/16 / #20160053311

Multiplex y-str analysis

Novel y-str multiplex analysis designs, primer design, allelic ladders, methods of use and kits are disclosed, including the use of primer sets designed to provide amplicons for at least 11 y-str loci having a base pair size of less than about 220 bp, as well as the use of primer sets designed to provide amplicons for at least 22 y-str loci including at least 5 rapidly mutating loci.. . ... Life Technologies Corporation

02/18/16 / #20160047747

Systems and methods for genetic sequencing

A device including a transparent layer defining a surface exposed to a flow volume and to secure a target polynucleotide template and a detector structure secured to the transparent layer and including a plurality of detectors to detect a signal emitted during nucleotide incorporation along the target polynucleotide template.. . ... Life Technologies Corporation

02/18/16 / #20160046979

Methods and compositions for multiplex pcr

The present invention provides methods, compositions, kits, systems and apparatus that are useful for determining copy number variation of one or more nucleic acids present in a sample. In some aspects, the method includes various target-specific primers that allow for the selective amplification of one or more target nucleic acids in the sample. ... Life Technologies Corporation

02/18/16 / #20160045600

Compositions and methods for efficient delivery of molecules to cells

The present invention provides lipid aggregate compositions having at least two cationic lipids in combination with one or optionally more than one non-cationic lipids for the in vitro and/or in vivo delivery of biologically active molecules including nucleic acids, specifically dna and rna, and proteins into cells and tissues. Methods are also provided that use the compounds of the present invention to deliver biologically active molecules, into cells and tissues to facilitate the expression of target proteins therein. ... Life Technologies Corporation

02/11/16 / #20160040256

Methods, compositions, and kits for detecting allelic variants

In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as snps, or nucleotide (nt) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. ... Life Technologies Corporation

02/04/16 / #20160033449

Column adc

The described embodiments may provide a chemical detection circuit. The chemical detection circuit may comprise a column of chemically-sensitive pixels. ... Life Technologies Corporation

02/04/16 / #20160033448

Active chemically-sensitive sensors with reset switch

Methods and apparatus relating to fet arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.. ... Life Technologies Corporation

02/04/16 / #20160032389

Method, kits, and reaction mixtures for high resolution melt genotyping

Various methods are described that provide for high resolution melt (hrm) genotyping. Some embodiments comprise providing a locus specific primer, and two allele specific primers each comprising at least one single nucleotide polymorphism (snp) allele-hybridizable sequence, wherein at least one of the allele specific primers also comprises at least one nucleotide alteration. ... Life Technologies Corporation

02/04/16 / #20160032375

Nucleic acid amplification

In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. ... Life Technologies Corporation

02/04/16 / #20160032371

Matrix arrays and methods for making same

A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. ... Life Technologies Corporation

02/04/16 / #20160032262

Methods and compositions for pcr

A modified thermostable pol b dna polymerase, produced by a reaction, under essentially aqueous conditions, of a thermostable pol b dna polymerase and a modifier reagent of formula i wherein the reaction results in a thermally reversible inactivation of the thermostable pol b dna polymerase activity and the 3′-5′ exonuclease activity, which polymerase is suitable for hot-start pcr. Also disclosed are the method for the modification, a polynucleic acid amplification method and pcr reaction mixture and kit comprising the modified thermostable pol b dna polymerase.. ... Life Technologies Corporation

02/04/16 / #20160032251

Chimeric pestivirus with insertion in 3' nontranslated region (3' ntr) with stable replication and rnase resistance

The construction of a chimeric pestivirus by the identification of selected regions in the 3′ntr of the viral rna genome is described where additional rna sequences can be stably inserted. These sequence insertions in the viral rna genome were stable in replication and capable of forming infectious, rnase resistant virus particles. ... Life Technologies Corporation

02/04/16 / #20160031821

Sdp-containing heterobifunctional agents

The present disclosure is directed to a reactive ester agent for conjugating a click-reactive group to a carrier molecule or solid support. The reactive ester agent has the general formula ia, wherein the variables r1, r2, r3, ra and l are described throughout the application.. ... Life Technologies Corporation

01/28/16 / #20160028545

Systems and methods for identifying an individual

The present application relates to systems and methods using biometric data of an individual for identifying the individual and/or verifying the identity of an individual. These systems and methods are useful for, amongst many applications, more secure identification of high-risk individuals attempting to gain access to an entity, transport, information, location, security organization, law enforcement organization, transaction, services, authorized status, and/or funds.. ... Life Technologies Corporation

01/28/16 / #20160026760

Systems and methods for the determination of a copy number of a genomic sequence

System and methods for the determination of a copy number of a target genomic sequence; either a target gene or genomic sequence of interest, in a biological sample are described. Various methods utilize a model drawn from a probability density function (pdf) for the assignment of a copy number of a target genomic sequence in a biological sample. ... Life Technologies Corporation

01/28/16 / #20160026753

Systems and methods for analysis and interpretation of nucleic acid sequence data

Systems and method for annotating variants within a genome can call variants from reads or receive called variants directly and associate the called variants with functional annotations and interpretive annotations. A summary report of the called variants, the associated functional annotations, and the associated interpretive annotations can be generated.. ... Life Technologies Corporation

01/28/16 / #20160025713

Site-specific labeling of affinity tags in fusion proteins

The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula a(b)n, wherein a is a fluorophore, b is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. ... Life Technologies Corporation

01/28/16 / #20160025676

Compositions and methods for improving resolution of biomolecules separated on polyacrylamide gels

Gels, such as polyacrylamide gels, are provided that include linear polyacrylamide in the stacking gel. Native gels that include linear polyacrylamide in the stacker can be used to separate biomolecular complexes, such as protein complexes. ... Life Technologies Corporation

01/28/16 / #20160024494

Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis

The present invention relates to nucleic acid inhibitors, compositions and method for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. ... Life Technologies Corporation

01/28/16 / #20160024470

Methods and materials for the generation of regulatory t cells

Methods are disclosed for the generation of immunosuppressive regulatory t cells. The methods can include contacting a population of cd4+cd25− t cells with a t cell receptor (tcr)/cd3 activator, a tcr co-stimulator activator, and rapamycin. ... Life Technologies Corporation

01/28/16 / #20160024138

Method for deblocking of labeled oligonucleotides

The invention relates to a process for deblocking substantially a blocked, detectably labeled oligonucleotide by contacting the blocked detectably labeled oligonucleotide with an effective amount of a nucleophilic amino compound under conditions that result in substantial deblocking of the oligonucleotide, thereby giving the substantially deblocked oligonucleotide.. . ... Life Technologies Corporation

01/28/16 / #20160023784

Methods and apparatus for processing fluids

A method for processing a fluid includes removably securing a retention member to a vessel that bounds a chamber; inserting a collapsible bag within the chamber of the vessel; securing the bag to the retention member so that the bag is supported within the chamber of the vessel; and dispensing a fluid into a compartment of the collapsible bag supported within the chamber of the vessel. The fluid can be mixed within bag while the bag is disposed within the vessel.. ... Life Technologies Corporation

01/21/16 / #20160019340

Systems and methods for detecting structural variants

Systems and method for identifying gene fusions can obtain sequencing information for a plurality of amplicons from a nucleic acid sample. The sequencing information can include a plurality of reads that are initially partially mapped to a reference sequence. ... Life Technologies Corporation

01/21/16 / #20160017394

Compositions and methods for nucleic acid assembly

The present disclosure generally relates to compositions and methods for the assembly of nucleic acid molecules into larger nucleic acid molecules. Also provided are compositions and methods for seamlessly connection of nucleic acid molecules with high sequence fidelity.. ... Life Technologies Corporation

01/21/16 / #20160017391

Cdna synthesis improvements

The present invention generally relates to methods of making cdna molecules and cdna libraries. The invention also relates to cdna molecules and cdna libraries produced according to these methods, as well as to vectors and host cells containing such cdna molecules and libraries. ... Life Technologies Corporation

01/21/16 / #20160017217

Magnesium-based coatings for nanocrystals

Semiconductor nanocrystal compositions comprising magnesium containing shells and methods of preparing them are described. The compositions provide strong emission in the blue and green wavelengths as well as chemical and photostability that have not been achieved with conventional shell materials.. ... Life Technologies Corporation

01/14/16 / #20160011147

Expanding cam lock for sealing slab gels in an electrophoresis apparatus

An expanding cam lock for use with an electrophoresis system is disclosed herein. In one example embodiment, the expanding cam lock includes a base plate with a first surface adapted to engage the first buffer core assembly and a follower plate having second surface adapted to engage the second buffer core assembly, buffer dam or buffer displacement dam. ... Life Technologies Corporation

01/14/16 / #20160011146

Sensor arrays and methods for making same

A system includes a sensor including a sensor pad and includes a well wall structure defining a well operatively connected to the sensor pad. The sensor pad is associated with a lower surface of the well. ... Life Technologies Corporation

01/14/16 / #20160011145

Integrated sensor arrays for biological and chemical analysis

The invention is directed to apparatus and chips comprising a large scale chemical field effect transistor arrays that include an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid for analysis. In one aspect such transistor arrays have a pitch of 10 μm or less and each sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region. ... Life Technologies Corporation

01/14/16 / #20160011115

Optical systems and methods for biological analysis

An instrument for processing and/or measuring a biological process contains a sample processing system, an excitation source, an excitation optical system, an optical sensor, and an emission optical system. The sample processing system is configured to retain a first sample holder and a second sample holder, wherein the number of sample cells is different for each sample holder or a characteristic dimension for the first sample cells is different from that of the second sample holder. ... Life Technologies Corporation

01/14/16 / #20160011085

Simultaneous acquisition of biometric data and nucleic acid

Systems, methods, and kits are disclosed for collection, labeling and analyzing biological samples containing nucleic acid in conjunction with collecting at least one ridge and valley signature of an individual. Such devices and methods are used in forensic, human identification, access control and screening technologies to rapidly process an individual's identity or determine the identity of an individual.. ... Life Technologies Corporation

01/14/16 / #20160010153

Novel compositions, methods and kits for blood typing

The present disclosure provides compositions, methods, and kits for typing of blood groups by their genotype. In some embodiments, the compositions, methods, and kits include one or more genotype reference nucleic acids.. ... Life Technologies Corporation

01/14/16 / #20160010149

Integrated sensor arrays for biological and chemical analysis

An apparatus comprising a chemical field effect transistor array in a circuit-supporting substrate is disclosed. The transistor array has disposed on its surface an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid. ... Life Technologies Corporation

01/14/16 / #20160010146

Se33 mutations impacting genotype concordance

Disclosed are primer set compositions, methods and kits for human identification using the highly complex sequence locus, se33 (actbp2) in single and multiplex pcr reactions. Additionally, disclosed are three newly discovered single nucleotide polymorphisms (snps) within the se33 locus that can cause discordance seen as mobility shift or allelic dropout. ... Life Technologies Corporation

01/14/16 / #20160010145

Detection of rna with substituted unsymmetrical cyanine dyes

Substituted unsymmetrical cyanine dyes are provided herein, including kits, assays systems and methods for their use in the detection of rna compounds (e.g., mirna).. . ... Life Technologies Corporation

01/14/16 / #20160010113

Compositions and methods for engineering cells

The disclosure relates generally to genetic manipulation of stem and primary cells and to reprogramming of somatic cells, more specifically, human cells. In particular, compositions and methods are disclosed for the generation and maintenance of such engineered cells.. ... Life Technologies Corporation

01/14/16 / #20160008776

Drive shaft locking cap and related mixing system and method

A mixing system includes a housing having a motor mount rotatably coupled thereto, the motor mount having a passage extending therethrough. A drive shaft is removably positioned within the passage of the motor mount. ... Life Technologies Corporation

01/07/16 / #20160003768

Surface treatment of semiconductor sensors

A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. ... Life Technologies Corporation

01/07/16 / #20160003743

Device and methods for quantifying analytes

The present invention relates to devices and methods for measuring the quantity of multiple analytes in a sample. The device is designed such that each of the analyte sensing elements is configured to measure the quantity of a predetermined analyte and where the machine executable instructions are configured to select the proper analyte sensing element corresponding to the analyte to be measured.. ... Life Technologies Corporation

01/07/16 / #20160003717

Laser capture microdissection (lcm) extraction device and device carrier, and method for post-lcm fluid processing

The present invention generally discloses an extraction system that provides a locale for fluid processing and extraction on a post-microcapture transfer film. The extraction system includes a transfer film carrier and an extraction device forming a reservoir. ... Life Technologies Corporation

01/07/16 / #20160002723

Conjugated polymeric particle and method of making same

A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.. . ... Life Technologies Corporation

01/07/16 / #20160001249

Methods for loading a sensor substrate

A method of loading beads on a sensor substrate includes applying a suspension including beads to a flow cell defined over a sensor substrate. The sensor substrate includes a plurality of wells. ... Life Technologies Corporation








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