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Life Technologies Corporation patents (2017 archive)


Recent patent applications related to Life Technologies Corporation. Life Technologies Corporation is listed as an Agent/Assignee. Note: Life Technologies Corporation may have other listings under different names/spellings. We're not affiliated with Life Technologies Corporation, we're just tracking patents.

ARCHIVE: New 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 | Company Directory "L" | Life Technologies Corporation-related inventors


Nucleotide transient binding for sequencing methods

Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (snp) analyses. ... Life Technologies Corporation

Hydrazinyl and aminooxy compounds and their methods of use

The present disclosure is directed dye compounds containing a hydrazinyl substituent and optionally, one or more negatively charged groups, such as sulfonate, phosphate, phosphonate, and/or carboxylate groups and dye compounds containing an aminooxy substitutent. The compounds are useful in the detection of analytes containing aldehyde and ketone groups, including, for example, glycans.. ... Life Technologies Corporation

Novel compositions, methods and kits for microorganism detection

Methods, compositions and kits for detecting microorganisms and/or profiling microbiota such as for example through use of nucleic acid amplification and detection.. . ... Life Technologies Corporation

Labeling and detection of post translationally modified proteins

Provided in certain embodiments are new methods for forming azido modified biomolecule conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling a biomolecules with an azide group.. ... Life Technologies Corporation

Acoustically settled liquid-liquid sample purification system

A sample purification system includes a container assembly bounding a sample purification compartment and having an upper end and an opposing lower end, the sample purification compartment comprising mixing zones and settling zones. A plurality of shielding elements are positioned within the sample purification compartment so as to at least partially separate adjacent mixing zones and settling zones or separate adjacent mixing zones, the mixing zones being in fluid communication with the settling zones. ... Life Technologies Corporation

Methods and systems for designing gene panels

A system and method of selecting genes for a gene panel, includes retrieving gene-disease associations of genes associated with diseases at a given level in the disease hierarchy from a disease association database. The disease association database stores disease information, gene information, phenotype information, associations between diseases in the disease hierarchy, gene-disease associations and strength parameters related to the gene-disease associations. ... Life Technologies Corporation

Method of culturing a cell with a supplement

Cell culture media, concentrated media and feeds, methods of manufacturing cell culture media and feeds, and methods of culturing cells are provided. One or more small peptides, including dipeptides are added to the cell culture media to provide improved stability and improved conditions for culturing cells.. ... Life Technologies Corporation

Inventory management system and method of use

Provided is a computer-implemented method for product management the method can include obtaining user identification information from a user, wherein the identification code is associated with a financial account, of the user; validating, by a processor, that the identification information is authentic based on registration information that was previously received; providing first access signal to a locking mechanism of a locking enclosure to unlock the locking mechanism based on the validating, wherein the locking enclosure securely stores a purchasable product; obtaining product identification information from a scan of a bar code associated with the product providing the product information that was obtained to be rendered on a display; receiving an indication from the user that the product information is correct; and providing a second access signal to the locking mechanism to lock the locking mechanism based on the indication that was received.. . ... Life Technologies Corporation

Systems and methods for identifying sequence variation

Systems and method for determining variants can receive mapped reads, align flow space information to a flow space representation of a corresponding portion of the reference. Reads spanning a position with a potential variant can be evaluated in a context specific manner. ... Life Technologies Corporation

Se33 mutations impacting genotype concordance

Disclosed are primer set compositions, methods and kits for human identification using the highly complex sequence locus, se33 (actbp2) in single and multiplex pcr reactions. Additionally, disclosed are three newly discovered single nucleotide polymorphisms (snps) within the se33 locus that can cause discordance seen as mobility shift or allelic dropout. ... Life Technologies Corporation

Anti-viral azide containing compounds

Methods of using azide-modified biomolecules, such as fatty acids, carbohydrates and lipids, to treat a plant, an insect or an animal infected with a virus or to inhibit infectivity of a virus, such as the human immunodeficiency virus, are provided. Also provided are methods of labeling a virus, such as human immunodeficiency virus, with an azide-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. ... Life Technologies Corporation

Systems, methods, and apparatuses for image capture and display

Provided are systems and methods that allow a user to capture images at low- and high-level magnification and then overlay the high-level magnification images on the low-level magnification image to ease review of the images. The high-level magnification images may be overlaid on the low-level magnification image based at least in part on the portion of the low-level magnification image from which the high-level image was originated.. ... Life Technologies Corporation

Expanding cam lock for sealing slab gels in an electrophoresis apparatus

An expanding cam lock for use with an electrophoresis system is disclosed herein. The cam lock allows the simultaneous use of multiple slab gel cassettes in first and second buffer core assemblies in an electrophoresis system while maintaining the necessary compressive force to create a liquid-tight seal between the anode and cathode buffer solutions. ... Life Technologies Corporation

Penta e polymorphisms for human identification

Disclosed herein are methods and compositions for human identification using polymorphisms in the penta e short tandem repeat locus. These newly disclosed polymorphisms are significant in preventing allelic drop out.. ... Life Technologies Corporation

11/09/17 / #20170321271

Combinatorial barcode sequences, and related systems and methods

A kit for use with a nucleic acid sequencing instrument can include a plurality of combinatorial barcodes sequences meeting the following criteria: each of the combinatorial barcode sequences comprise a plurality of iterations of a sequence motif, where the sequence motif comprises a first nucleotide base from a first group of nucleotide bases followed by a second nucleotide base from a second group of nucleotide bases, the first group and the second group differing from each other; and the plurality of combinatorial barcode sequences is at least 1,000,000 different barcode sequences.. . ... Life Technologies Corporation

11/09/17 / #20170321269

Method of determining the nucleotide sequence of oligonucleotides and dna molecules

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of dna polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a dna fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. ... Life Technologies Corporation

11/09/17 / #20170321245

Flash and glow 1,2-dioxetanes

Compounds having chemiluminescent flash and glow properties. Also disclosed are methods using the compounds to generate light, detect and/or quantify enzymes, antigens, and/or nucleic acids. ... Life Technologies Corporation

11/09/17 / #20170321219

Oligonucleotide compositions with enhanced efficiency

The oligonucleotide compositions of the present invention make use of combinations of oligonucleotides. In one aspect, the invention features an oligonucleotide composition including at least 2 different oligonucleotides targeted to a target gene. ... Life Technologies Corporation

10/26/17 / #20170307529

Optical systems and methods for biological analysis

An instrument for processing and/or measuring a biological process contains an excitation source, a sample holder, an optical sensor, an excitation optical system, and an emission optical system. The sample holder is configured to receive a plurality of biological samples. ... Life Technologies Corporation

10/26/17 / #20170307447

Probe port and realted container system

A container system includes a flexible bag, a probe port, and a probe. The probe port includes an elongated tubular member having an interior surface bounding a first passageway extending between a first end and an opposing second end, the tubular member being made of a polymeric or elastomeric material and being flexible. ... Life Technologies Corporation

10/26/17 / #20170306306

Compositions and methods for enhancing homologous recombination

The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.. ... Life Technologies Corporation

10/26/17 / #20170306072

Particles containing multi-block polymers

Polymer particles having a multi-block vinylic polymer attached to their surface are disclosed. The particles can be used in a variety of purification and detection methods.. ... Life Technologies Corporation

10/26/17 / #20170305954

Synthesis of 2', 3'-dideoxynucleosides for automated dna synthesis and pyrophosphorolysis activated polymerization

Methods for preparation of 2′,3′-dideoxynucleotides support structures, such as 2′,3′-dideoxyguanosine, 2′,3′-dideoxyadenosine, and 3′-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated dna synthesis and pyrophosphorolysis activated polymerization.. ... Life Technologies Corporation

10/12/17 / #20170293713

Models for analyzing data from sequencing-by-synthesis operations

A method of modeling a background signal when sequencing a polynucleotide strand using sequencing-by-synthesis includes: flowing a series of nucleotide flows onto a reactor array having multiple reaction confinement regions, one or more copies of the polynucleotide strand being located in a loaded reaction confinement region of the reactor array, the loaded reaction confinement region being located in a vicinity of one or more neighboring reaction confinement regions that may or may not be loaded; receiving output signals from the reactor array; and modeling a background signal for the loaded reaction confinement region using the received output signals and a model adapted to account at least for an exchange of ions between the one or more neighboring reaction confinement regions and a headspace adjacent the loaded reaction confinement region and the one or more neighboring reaction confinement regions.. . ... Life Technologies Corporation

10/12/17 / #20170292156

Modified proteins and methods of making and using same

Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pka values are described. ... Life Technologies Corporation

10/12/17 / #20170292144

Compositions, methods, and kits for synthesis and detection of nucleic acids

Compositions, methods, and kits for synthesizing, detecting, and/or quantifying nucleic acids are provided herein. Embodiments comprise a nucleic acid amplification composition comprising a thermostable dna polymerase and agents which improve nucleic acid synthesis, amplification, detection, and/or quantification of nucleic acid targets in a crude extract or crude lysate sample.. ... Life Technologies Corporation

10/05/17 / #20170284907

Automated microdissection instrument

Systems and methods for automated laser microdissection are disclosed including automatic slide detection, position detection of cutting and capture lasers, focus optimization for cutting and capture lasers, energy and duration optimization for cutting and capture lasers, inspection and second phase capture and/or ablation in a quality control station and tracking information for linking substrate carrier or output microdissected regions with input sample or slide.. . ... Life Technologies Corporation

10/05/17 / #20170283868

Methods and apparatus for single molecule sequencing using energy transfer detection

Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. ... Life Technologies Corporation

09/28/17 / #20170275688

Methods, compositions and systems for sample deposition

Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. ... Life Technologies Corporation

09/28/17 / #20170275674

Next-generation sequencing quality control monitoring tool

Systems and methods are used to provide an online tool for clinical laboratories to monitor next-generation sequencing (ngs) workflow using quality control (qc) material that can be used for multiple assays. The tool utilizes a highly multiplexed qc with ngs assays that detect somatic mutations. ... Life Technologies Corporation

09/21/17 / #20170269109

Method and apparatus for automated sample manipulation

The present teachings provide apparatuses and methods for automated handling of samples, e.g., biological or chemical samples. The apparatuses and the methods of the present teachings allow automated performance of various sample manipulation steps without manual intervention. ... Life Technologies Corporation

09/14/17 / #20170260512

Polymerase compositions, methods of making and using same

The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, modified polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. ... Life Technologies Corporation

09/07/17 / #20170254775

Isotopically-labeled proteome standards

The invention provides methods for quantifying biomolecules, such as polypeptides in mass spectrometric analysis. The methods include use of a biomolecule standard having at least one atomic isotope different than that of the naturally occurring isotopes in the biomolecule of interest. ... Life Technologies Corporation

09/07/17 / #20170253922

Human identification using a panel of snps

The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex pcr of one or more nucleic acids which belong to a panel of single nucleotide polymorphisms (snps) useful to identify a human. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences in the panel. ... Life Technologies Corporation

09/07/17 / #20170253921

Methods, kits & compositions for determining gene copy numbers

The present specification relates to methods, compositions, and kits for determining the presence of the number of copies of a gene. The present disclosure provides methods and compositions to determine zygosity in transgenic plants, number of transgenes in a plant or animal, karyotyping, determine cnv in human and animal samples, diagnose and detect diseases and conditions that are characterized by having variations in the number of copies of one or more genes.. ... Life Technologies Corporation

08/31/17 / #20170248606

Apparatuses, methods, computer program products, and kits for hi-throughput glycan analysis

An apparatus for glycan analysis is disclosed. The apparatus includes a plurality of loading wells adapted to receive a plurality of samples; a plurality of capillaries arranged in correspondence with the loading wells, each of the capillaries including a first portion including a stacking gel and a second portion including a resolving gel; and a plurality of eluting wells arranged in correspondence with the capillaries and adapted to receive a portion of the samples having traversed the capillaries.. ... Life Technologies Corporation

08/31/17 / #20170247756

Genetic sequence verification compositions, methods and kits

Methods, compositions and kits are described for resequencing, confirming or verifying next generation sequencing (ngs) results with sanger sequencing. These methods are particularly useful for samples having very limited quantities such as formalin-fixed, paraffin-embedded (ffpe), laser capture microdissection (lcm), fine needle biopsies or aspirates.. ... Life Technologies Corporation

08/31/17 / #20170247504

Dibenzosilole monomers and polymers and methods for their preparation and use

Water-soluble, conjugated polymers containing one or more dibenzosilole monomer residues, as well as compositions, kits, and methods of making and using such polymers are disclosed. Also disclosed are dibenzosilole derivatives substituted with one or more water-solubilizing groups, and methods of making and using such derivatives to prepare water-soluble dibenzosilole polymers.. ... Life Technologies Corporation

08/31/17 / #20170246603

Gas spargers and related container systems

A container system includes a collapsible bag having an interior surface at least partially bounding a chamber, the chamber being adapted to hold a fluid. A sparger is disposed within the chamber of the bag, the sparger bounding a compartment and having an inside edge that encircles an opening passing through the sparger. ... Life Technologies Corporation

08/24/17 / #20170241943

Methods for calibrating an array of chemically-sensitive sensors

Methods and apparatus relating to very large scale fet arrays for analyte measurements. Chemfet (e.g., isfet) arrays may be fabricated using conventional cmos processing techniques based on improved fet pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. ... Life Technologies Corporation

08/17/17 / #20170235874

Methods and systems for detecting minor variants in a sample of genetic material

A computer-implemented method for determining minor variants. The method includes receiving electropherogram sequence data from a test sample, identifying any non-primary peaks in the electropherogram, and characterizing identified non-primary peaks using at least one signal feature. ... Life Technologies Corporation

08/10/17 / #20170225169

Thermal uniformity for thermal cycler instrumentation using dynamic control

A method for performing polymerase chain reactions (pcr) for improving thermal non-uniformity is provided. The method includes measuring a first temperature, by a first sensor, of a first sample block sector of a sample block and measuring a second temperature, by a second sensor, of a second sample block sector of the sample block that is adjacent to the first sample block sector. ... Life Technologies Corporation

08/03/17 / #20170218095

Monodisperse submicron polymer particles

This invention relates to monodisperse cross-linked polymer particles, comprising particles with a substantially smooth outer surface and an average diameter of less than 1 μm, wherein the particles are solid or porous, and wherein the coefficient of variation (cv) % of the particles, when measured by cps disk centrifugation analysis, is less than 15%. These monodisperse cross-linked polymer particles may comprise magnetic material and are useful in various application. ... Life Technologies Corporation

07/20/17 / #20170206313

Using flow space alignment to distinguish duplicate reads

Systems and method for identifying duplicate reads can receive first and second reads, determine if the first and second reads have a same start and end position, determine a binary flow difference, and identify the second read as a duplicate of the first read when the binary flow difference exceeds a threshold.. . ... Life Technologies Corporation

07/20/17 / #20170204398

Nucleic acid preparation method

A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support whilst maintaining it on the support. ... Life Technologies Corporation

07/13/17 / #20170199734

Systems and methods for versioning hosted software

Systems and methods for performing a validated analysis can include access to first and second versions of an analysis engine. The first version of the analysis engine can be used to perform a previously validated analysis. ... Life Technologies Corporation

07/13/17 / #20170197185

Container with film sparger

A container assembly includes a flexible bag having an interior surface bounding a chamber and an opposing exterior surface, the bag having a bottom end wall being comprised of a first sheet overlying a second sheet, the first sheet and the second sheet of the bottom end wall being welded together so as to form a first sparging area and a separated second sparging area that are both bounded between the first sheet and the second sheet, at least a portion of the first sheet overlying the first and second sparging areas being gas permeable.. . ... Life Technologies Corporation

07/06/17 / #20170194985

Methods and computer program products for compression of sequencing data

A compression method includes measuring a waveform associated with a chemical event occurring on a sensor array, wherein the waveform comprises at least one region associated with expected measured values and at least one region associated with unpredictable measured values; applying a first compression process to the waveform, the first compression process including an averaging of one or more frames in one or more portions of the waveform; applying a keyframe delta compression to the frame-averaged data; forming a compressed data structure including a keyframe and calculated differences subsequent to the keyframe; and storing one or more compressed data structures in memory.. . ... Life Technologies Corporation

07/06/17 / #20170191922

System and method for providing stable fluid flow

An embodiment of a system with a minute measure of pulsatility in a flow of a fluid is described that comprises a first pump configured to flow the fluid to a junction at a first flow rate that comprises a measure of pulsatility; and a second pump configured to flow a portion of the fluid from the junction at a second flow rate that is less than the first flow rate to produce a flow of the fluid at a third flow rate from the junction with a minute measure of pulsatility.. . ... Life Technologies Corporation

07/06/17 / #20170191571

Pinch flow regulator

A valve for regulating fluid flow includes a housing base defining a lower cavity and comprising a pinch structure within the lower cavity, a gas inlet providing external access to the lower cavity, a base fluid inlet, and a base fluid outlet. A housing cover defines an upper cavity and comprises a cover fluid inlet and a cover fluid outlet. ... Life Technologies Corporation

07/06/17 / #20170191051

Labeled enzyme compositions, methods and systems

Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are labeled polymerase conjugates including a polymerase linked to one or more labels, wherein the conjugate has polymerase activity. ... Life Technologies Corporation

06/29/17 / #20170186840

Nanocrystals with high extinction coefficients and methods of making and using such nanocrystals

A population of bright and stable nanocrystals is provided. The nanocrystals include a semiconductor core and a thick semiconductor shell and can exhibit high extinction coefficients, high quantum yields, and limited or no detectable blinking.. ... Life Technologies Corporation

06/29/17 / #20170183617

Fluid mixing system with laterally displaced flexible drive lines and methods of use

A fluid mixing system includes a container bounding a compartment and extending between a first end and an opposing second end. An elongated first drive line and second drive line are disposed within the compartment of the container and are rotatable therein. ... Life Technologies Corporation

06/29/17 / #20170182496

Systems and methods for transfer of liquid samples

A system for preparing biological sample contains a body including a proximal side and a distal side, a plurality of mandrels, a plurality of resilient elements, a plurality of fluid dispensers, and one or more samples. The mandrels are moveably positioned within the body, where each resilient element engages a respective one of the mandrels. ... Life Technologies Corporation

06/29/17 / #20170182492

Integrated system for nucleic acid amplification and detection

Devices, systems, methods, and kits are disclosed for amplifying small quantities of nucleic acid and identifying the nucleic acid sequences of the amplified nucleic acid products.. . ... Life Technologies Corporation

06/22/17 / #20170177792

Methods and systems for knowledge discovery using biological data

A system for analyzing biological data, comprising: a storage configured to store a plurality of data files containing biological data obtained from a plurality of devices; a server configured to: host a plurality of applications, each configured to be implemented on the server and to provide analysis, manipulation, comparison, visualization, or a combination thereof, of the biological data included in the data files, wherein the plurality of applications allow a user to analyze different data files related to the same sample and compare the results of the analysis.. . ... Life Technologies Corporation

06/22/17 / #20170177791

Methods and systems for pcr quantitation

A method for quantifying nucleic acid is provided. The method includes determining a first reference threshold cycle for a first predetermined input quantity for a reference nucleic acid, determining a first target threshold cycle for the first predetermined input quantity for a target nucleic acid, determining a second reference threshold cycle for a second predetermined input quantity for the reference nucleic acid, and determining a second target threshold cycle, by the processor, for the second predetermined input quantity for the target nucleic acid. ... Life Technologies Corporation

06/22/17 / #20170176385

Capillary array cartridge for capillary electrophoresis systems

The present disclosure relates, in some embodiments, to an apparatus for conducting a capillary electrophoresis assay. The apparatus can comprise a capillary array comprising an anode end and a cathode end, the capillary array provided in a housing further comprising a reservoir configured to house a separation medium and an anode buffer. ... Life Technologies Corporation

06/22/17 / #20170176384

Apparatuses, systems and methods for sequencing using capillary electrophoresis

In one aspect, a biological sequencing device comprising a cartridge configured to be removed from the instrument is disclosed. In various embodiments the cartridge can include one or more capillaries suitable for capillary electrophoresis, a reservoir and a pump. ... Life Technologies Corporation

06/22/17 / #20170175171

Methods and kits for breaking emulsions

The disclosure relates generally to methods, kits and systems for extracting biomolecules from a water-in-oil emulsion, including breaking a water-in-oil emulsion comprising a plurality of aqueous droplets in a continuous hydrophobic fraction using a breaking solution to produce a resulting reaction mixture containing one or more biomolecules and manipulating the resulting reaction mixture to form at least two phases, where one of the phases includes an aqueous phase containing the one or more biomolecules.. . ... Life Technologies Corporation

06/22/17 / #20170175092

Mutant rb69 dna polymerase

Provided herein are mutant dna-dependent polymerases which are derived from, or otherwise related to, wild type rb69 dna polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. ... Life Technologies Corporation

06/22/17 / #20170174846

Purification systems and methods

A method of preparing hydrogel particles includes applying a solution including a plurality of hydrogel particles to a stir cell. A retentate side of a filter defines a lower surface of the stir cell. ... Life Technologies Corporation

06/22/17 / #20170174700

Biotin derivatives

Biotin derivatives, methods of using the biotin derivatives and kits comprising the biotin derivatives.. . ... Life Technologies Corporation

06/22/17 / #20170173587

Systems and methods for a thermal cycler heated cover

A thermal cycler system for use with a sample holder configured to receive a plurality of samples includes a sample block configured to receive the sample holder, a cover lid configured to move in a direction toward the sample block from an open position to a closed position, a heated cover operatively coupled to the cover lid and configured to move in a direction toward the sample block from a raised position to a first lowered position, in which the heated cover contacts the sample holder when the sample holder is received by the sample block, and a drive assembly including a motion guide configured to move in a direction toward the sample block from a first position, wherein the cover lid is in the open position and the heated cover is in the raised position, to a second position, wherein the cover lid is in the closed position.. . ... Life Technologies Corporation

06/22/17 / #20170173586

Thermal cycler systems and methods of use

A thermal cycler system for use with a sample holder configured to receive a plurality of samples includes a sample block having an upstanding peripheral side wall and being configured to receive the sample holder and an adaptor having an upstanding peripheral side wall configured to be positioned about the peripheral side wall of the sample block. When the peripheral side wall of the adaptor is positioned about the peripheral side wall of the sample block and the sample holder is received in the sample block, the peripheral side wall of the adaptor extends in an upward direction toward the sample holder.. ... Life Technologies Corporation

06/15/17 / #20170166943

Oligosaccharide modification and labeling of proteins

The present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified oligosaccharides. The invention further relates to kits that may be used to produce the antibodies of the invention.. ... Life Technologies Corporation

06/15/17 / #20170166854

Pipette tip for electroporation device

Provided herein is a pipette tip for electroporation including an outer surface, a void located within the outer surface, and a conductor located at least partially on or within the outer surface, in electrical communication with at least a portion of the outer surface and the void. Further provided herein is a pipette tip for electroporation including a body, a connector, and an elongated part. ... Life Technologies Corporation

06/15/17 / #20170166603

Large stokes shift dyes

Provided herein are heptamethine cyanine dyes having a large stokes shift, and the salts and conjugates thereof. Also provided are methods of using and making such large stokes shift dyes as fluorescence resonance energy transfer (fret) acceptors or donors.. ... Life Technologies Corporation

06/08/17 / #20170161467

Apparatus for collecting fingerprints and buccal swabs

Disclosed are devices and methods for collection, labeling and matching biological samples containing nucleic acid in conjunction with collecting at least one ridge and valley signature such as a fingerprint or footprint of an individual. Such devices and methods are used in forensic, human identification, paternity, tissue typing, and screening technologies to rapidly process an individual's identity, determine the identity of an individual along with the genotype profile of the individual.. ... Life Technologies Corporation

06/08/17 / #20170161429

Methods and systems for streamlining optical calibration

A method for calibrating a biological instrument is provided. The method comprises the steps of acquiring an image of at least one biological sample array, determining a first region of interest within the image, wherein the first region of interest comprises a first plurality of locations on the at least one biological array; and identifying within the first region of interest, a plurality of image elements associated with each of the first plurality of locations on the at least one biological array.. ... Life Technologies Corporation

06/08/17 / #20170160294

Methods of reversibly binding a biotin compound to a support

Methods of reversal of the binding between a biotin compound and a biotin-binding compound are disclosed. A method of reversibly releasing a biotinylated moiety from a streptavidin (or avidin) coated support is shown as an example. ... Life Technologies Corporation

06/08/17 / #20170160284

Protein melt analysis using dipyrrometheneboron difluoride compounds

According to the present teachings, systems, compositions, kits and methods for protein melt analysis are provided that utilizing a dye that is a dipyrrometheneboron difluoride compound. In some embodiments, a method comprises preparing a sample by mixing at least one protein with a dye, and applying a controlled heating, while recording the fluorescence emission of the sample.. ... Life Technologies Corporation

06/08/17 / #20170160230

Expanding cam lock for sealing slab gels in an electrophoresis apparatus

An expanding cam lock for use with an electrophoresis system is disclosed herein. The cam lock allows the simultaneous use of multiple slab gel cassettes in first and second buffer core assemblies in an electrophoresis system while maintaining the necessary compressive force to create a liquid-tight seal between the anode and cathode buffer solutions. ... Life Technologies Corporation

06/08/17 / #20170157538

System and method for emulsion breaking

A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.. . ... Life Technologies Corporation

06/01/17 / #20170153204

Preparation of electrophoresis gels, and related devices, systems, and methods

An apparatus for preparation of an electrophoresis slab gel may include a base having an opening configured to receive a cassette configured to contain an electrophoresis slab gel, a clamping mechanism configured to move relative to the base between an open position in which the clamping mechanism permits insertion of a cassette into the base, and a closed position in which the clamping mechanism is configured to clamp a cassette received in the base, a compressible pad operatively coupled to the clamping mechanism in a position to compress against a cassette received in the base in the closed position of the clamping mechanism. The cassette may include a first plate and a second plate, and a spacer mechanism separate from each of the first and second plates, the spacer mechanism configured to be positioned between the inner faces of the first plate and the second plate. ... Life Technologies Corporation

06/01/17 / #20170153201

Chemical sensor with conductive cup-shaped sensor surface

A system includes a sensor including a sensor pad and a well wall structure defining a well operatively coupled to the sensor pad. The well is further defined by a lower surface disposed over the sensor pad. ... Life Technologies Corporation

06/01/17 / #20170152469

Reactor foam sensor systems and methods of use

A foam sensor system includes a container bounding a compartment. A foam sensor assembly is mounted on the container and includes: a base being secured to the container; a foam contact being spaced apart from the base and disposed within the compartment of the container, at least a portion of the foam contact having a first diameter; and a transition member extending between the base and the foam contact with at least a portion of the transition member being openly exposed within the compartment of the container, at least a portion of the transition member having a second diameter that is smaller than the first diameter, the foam contact and transition member being connected together so that an electrical signal can pass therethrough.. ... Life Technologies Corporation

05/18/17 / #20170140671

Surgery simulation system and method

. . A surgery simulation system and method is disclosed. The surgery simulation system includes a simulation platform provided with a physical model thereon for simulating a human organ for incising and/or piercing; a force feedback device provided with an actuator and a surgical instrument connection component thereon, the surgical instrument connection component being detachably connected to a surgical instrument; a three-dimensional tracker for tracking the surgical instrument; a display device for displaying a virtual organ and/or a medical image; a control module having an electrical signal connection with the force feedback device, the three-dimensional tracker and the display device, controlling the force feedback device according to the signal of the three-dimensional tracker, and displaying a corresponding image on the display device. ... Life Technologies Corporation

05/18/17 / #20170140093

Methods and systems for in silico design

Embodiments describe computer systems and computer programs for implementing biocad methods comprising one or more data models and one or more biocad tools, wherein the biocad tools enable users to design or refactor a biomolecule or to conduct a biological experiment in silico by user input of one or more components selected by the user from a database populated with information on components and scientific data of existing biomolecules and experiments. Data models of the programs are operable to manage development of the new biomolecule or experiment based on information in the databases. ... Life Technologies Corporation

05/18/17 / #20170137866

Methods and compositions for labeling nucleic acids

The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. ... Life Technologies Corporation

05/18/17 / #20170137816

Double-stranded oligonucleotides

Antisense sequences, including duplex rnai compositions, which possess improved properties over those taught in the prior art are disclosed. The invention provides optimized antisense oligomer compositions and method for making and using the both in in vitro systems and therapeutically. ... Life Technologies Corporation

05/11/17 / #20170132359

Systems and methods for identifying somatic mutations

Systems and method for identifying somatic mutations can receive first and second sequence information, determine if a variant present in the first sequencing information is also present in the second sequence information, and identify variants present in the first sequence information are somatic mutations when the variant is either not present in the second sequence information or the presence of the variant in the second sequence information is likely due to a sequencing error.. . ... Life Technologies Corporation

05/11/17 / #20170131316

Sampling probes, systems, apparatuses, and methods

A sampling system is provided. The sampling system includes a housing. ... Life Technologies Corporation

05/11/17 / #20170130264

Novel compositions, methods and kits for enhancing pcr specificity

The present disclosure provides novel primers and method for the detection of specific nucleic acid sequences. The primers and methods provided herein are useful in a wide variety of molecular biology applications and are particularly useful in allele-specific pcr.. ... Life Technologies Corporation

05/11/17 / #20170128515

Antimicrobial & antiviral composition

A method of reducing the number of microorganisms entering the nose and proliferating in the nasal cavity including application of a solution of an antimicrobial, antiviral and antifungal composition to the anterior vestibular region of the nares. The antimicrobial, antiviral and antifungal solution includes ethyl alcohol as an active ingredient. ... Life Technologies Corporation

05/04/17 / #20170124315

Systems and methods for identifying an individual

The present application relates to systems and methods using biometric data of an individual for identifying the individual and/or verifying the identity of an individual. These systems and methods are useful for, amongst many applications, more secure identification of high-risk individuals attempting to gain access to an entity, transport, information, location, security organization, law enforcement organization, transaction, services, authorized status, and/or funds.. ... Life Technologies Corporation

05/04/17 / #20170121766

Alternative nucleotide flows in sequencing-by-synthesis methods

A method of sequencing a polynucleotide strand can include providing the polynucleotide strand with a primer annealed thereto and a polymerase operably bound to the polynucleotide strand; successively exposing the polynucleotide strand to the flow of four different dntps according to a first predetermined ordering; and successively exposing the polynucleotide strand to the flow of four different dntps according to a second predetermined ordering, wherein the second predetermined ordering is different from the first predetermined ordering.. . ... Life Technologies Corporation

04/27/17 / #20170114391

Polymerization of nucleic acids using proteins having low isoelectric points

This disclosure relates to the use of one or more proteins (e.g., globular proteins) having a low isoelectric point and/or a limited number (e.g., zero) of modifying groups in nucleic acid polymerization and/or amplification reactions such as polymerase chain reaction (pcr).. . ... Life Technologies Corporation

04/27/17 / #20170114321

Methods for eliminating at least a substantial portion of a clonal antigen-specific memory t cell subpopulation

The present invention relates generally to methods for stimulating t cells, and more particularly, to methods to eliminate undesired (e.g., autoreactive, alloreactive, pathogenic) subpopulations of t cells from a mixed population of t cells, thereby restoring the normal immune repertoire of said t cells. The present invention also relates to compositions of cells, including stimulated t cells having restored immune repertoire and uses thereof.. ... Life Technologies Corporation

04/27/17 / #20170114317

Filter-based system and method for efficient capture of lysis of suspended cells

A cell lysis workflow involving a cell suspension that is passed through a filter in a first flow direction from a first side of the filter toward a second side thereof. The filter captures a plurality of cells on the first side. ... Life Technologies Corporation

04/20/17 / #20170108518

Modified nucleic acid binding cyanine dyes for the detection of reactive oxygen species

Disclosed herein are compounds, compositions, methods, and kits for detecting reactive oxygen species (ros) by conventional fluorescence microscopy, fluorescence spectroscopy, flow cytometry, and/or high content imaging. The compounds disclosed herein are novel reduced nucleic acid binding cyanine dyes, which dyes are probes for detecting ros and measuring oxidative stress in cells either in vitro and/or in vivo. ... Life Technologies Corporation

04/20/17 / #20170108466

Electrophoresis gel with extended shelf life and high performance

An electrophoresis gel with a gel buffer that includes a gel amine buffer, a primary gel ampholyte, and a conjugate gel ampholyte is disclosed herein. The conjugate gel ampholyte may be selected from threonine and serine.. ... Life Technologies Corporation

04/20/17 / #20170108464

System and method for forming microwells

A method of forming a sensor component includes forming a first layer over a sensor pad of a sensor of a sensor array. The first layer includes a first inorganic material. ... Life Technologies Corporation

04/20/17 / #20170107567

Sequence amplification with linear primers

The present disclosure relates to the amplification of target nucleic acid sequences for various sequencing and/or identification techniques. The use of these primers, as described herein, allows for the reduction in the amplification of nonspecific hybridization events (such as primer dimerization) while allowing for the amplification of the target nucleic acid sequences.. ... Life Technologies Corporation

04/20/17 / #20170107539

Ribonucleoprotein transfection agents

Provided herein are compositions and methods useful, inter alia, for the delivery of ribonucleoprotein complexes (e.g., cas9/guide rna complexes) into cells. The compositions and methods provided herein are particularly useful for the delivery of ribonucleoprotein complexes into pluripotent cells and lymphatic cells.. ... Life Technologies Corporation

04/20/17 / #20170105838

Rapid deployment artificial chordae tendinae system

An embodiment includes an apparatus comprising: a hollow needle with a narrowed inner diameter in a distal third of the needle; and a flexible chord coupled to proximal and distal conduits; wherein the distal conduit is in the needle and distal to the narrowed inner diameter and the proximal conduit is in the needle and proximal to the narrowed inner diameter; wherein: (a)(i) long axes of the proximal and distal conduits are substantially parallel to a long axis of the needle when the proximal and distal conduits are in the needle, and (a)(ii) the proximal and distal conduits are configured to rotate when deployed from the needle such that their long axes are not parallel to a long axis of the chord when the chord is fully extended. Other embodiments are described herein.. ... Life Technologies Corporation

04/13/17 / #20170103162

Methods for the determination of a copy number of a genomic sequence in a biological sample

Methods for the determination of a copy number of a target genomic sequence; either a target gene or genomic sequence of interest, in a biological sample are described. Various methods utilize a model drawn from a probability density function (pdf) for the assignment of a copy number of a target genomic sequence in a biological sample. ... Life Technologies Corporation

04/13/17 / #20170101668

Systems and methods for substrate enrichment

A method of separating bead substrates includes applying an emulsion to an emulsion-breaking solution. A dispersed phase of the emulsion includes an unbound polynucleotide, a first set of bead substrates and a second set of bead substrates. ... Life Technologies Corporation

04/06/17 / #20170097317

Chemical detection device having multiple flow channels

The described embodiments may provide a chemical detection circuit that may comprise a plurality of first output circuits at a first side and a plurality of second output circuits at a second side of the chemical detection circuit. The chemical detection circuit may further comprise a plurality of tiles of pixels each placed between respective pairs of first and second output circuits. ... Life Technologies Corporation

04/06/17 / #20170096711

Multi-copy reference assay

A method, comprising amplifying a nucleic acid sequence of interest in a sample comprising genomic dna of a subject; amplifying a reference nucleic acid sequence in the sample; quantifying the amplified sequence of interest relative to the amplified reference sequence; and determining a copy number of the sequence of interest from the relative quantified amplified sequence of interest. The reference sequence may have at least 80% sequence identity to at least one of seq id no:1-38, such as seq id no:1-13. ... Life Technologies Corporation

04/06/17 / #20170096648

Polymerase compositions and kits, and methods of using and making the same

The present disclosure provides compositions, methods, kits, systems and apparatus that are useful for nucleic acid polymerization. In particular, recombinant polymerases and biologically active fragments thereof are provided that allow for nucleic acid amplification. ... Life Technologies Corporation

04/06/17 / #20170095841

Method for treating a semiconductor device

A method of treating a sensor array including a plurality of sensors and an isolation structure, where a sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array and the isolation structure is disposed between the sensor pad and sensor pads of other sensors of the plurality of sensors, comprises exposing the sensor pad and the isolation structure to a non-aqueous organo-silicon solution including an organo-silicon compound and a first non-aqueous carrier; applying an acid solution including an organic acid and a second non-aqueous carrier to the sensor pad; and rinsing the acid solution from the sensor pad and the isolation structure.. . ... Life Technologies Corporation

03/30/17 / #20170088893

Nucleic acid adaptors and uses thereof

Provided herein are compositions, systems, methods, and kits for joining together the ends of one or more polynucleotides using at least one pair of blocking oligonucleotide adaptors. Blocking oligonucleotide adaptors can be used to reduce the formation of adaptor dimers or trimers (or higher-order concatemers) which can improve the yield of desirable polynucleotide-adaptor products in any recombinant nucleic acid workflow. ... Life Technologies Corporation

03/30/17 / #20170088879

Systems and methods for performing digital pcr

Systems and methods are described for quantifying a target nucleic acid. A sample comprising a target nucleic acid is segregated into a first plurality of the reaction volumes containing at least one target nucleic acid molecule and a second plurality of the reaction volumes containing no target nucleic acid molecules. ... Life Technologies Corporation

03/30/17 / #20170088806

Stirred tank reactor systems and methods of use

A reactor system includes a support housing having an interior surface bounding a chamber, the chamber having a vertically extending central longitudinal axis. A flexible bag is disposed within the chamber of the support housing and has an interior surface bounding a compartment. ... Life Technologies Corporation

03/30/17 / #20170088805

Heat exchanger system with flexible bag

A bag assembly for use with a heat exchanger includes a flexible bag having of one or more sheets of polymeric material, the bag having a first end that bounds a first compartment and an opposing second end that bounds a second compartment, a support structure being disposed between the first compartment and the second compartment so that the first compartment is separated and isolated from the second compartment. A first inlet port, a first outlet port, and a first drain port are coupled with the flexible bag so as to communicate with the first compartment. ... Life Technologies Corporation

03/23/17 / #20170081715

Fast pcr for str genotyping

Disclosed is a method of amplifying a nucleic acid sequence, wherein the method comprises subjecting a reaction mixture to at least one amplification cycle, wherein the reaction mixture comprises a double-stranded nucleic acid and at least two primers capable of annealing to complementary strands of the double-stranded nucleic acid and amplifying at least one short tandem repeat (str) using a family a dna polymerase in a fast pcr protocol having a two-step amplification cycle in 25 seconds or less. Also disclosed are real-time pcr methods using the two-step protocol and kits for str profiling using the fast pcr protocol.. ... Life Technologies Corporation

03/23/17 / #20170081712

Systems and methods for analysis of nucleic acids

A system for detecting one or more target molecules includes a reaction device and a temperature controller. The reaction device comprises a plurality of reaction sites and plurality of field effect transistors corresponding to the plurality of reaction sites. ... Life Technologies Corporation

03/23/17 / #20170081706

Polymerization of nucleic acids using activation by polyphosphorolysis (app) reactions

This disclosure relates to methods of performing activation by polyphosphorolysis (app) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or μ-monothiopyrophosphate), and related compounds.. . ... Life Technologies Corporation

03/16/17 / #20170073746

Development of novel detergents for use in pcr systems

This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (pcr). Methods for preparing the modified detergents are also described.. ... Life Technologies Corporation

03/16/17 / #20170073359

Optically-detectable enzyme substrates and their method of use

The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. ... Life Technologies Corporation

03/16/17 / #20170072398

Systems and methods for biological analysis

A thermal block assembly for use in a biological analysis system includes a sample block, a heating and cooling element, a heat sink including a surface, the surface including a plurality of projections for engaging the heating and cooling element to hold the heating and cooling element on the heat sink. A thermal block assembly for use in a biological analysis system includes a heating and cooling element, a sample block including a lower surface configured to be thermally coupled to the heating and cooling element, one or more temperature sensors configured to extend through the one or more slots of the lower surface of the sample block, and one or more thermal pads between the one or more temperature sensors and heating and cooling element.. ... Life Technologies Corporation

03/09/17 / #20170068779

Biological database incorporating content-provider payment mechanism

Systems and methods are used to provide an aggregated genomic information database and compensate contributors. Genomic data and contributor data are received from a contributor client device and stored in an electronic database using a server computer. ... Life Technologies Corporation

03/09/17 / #20170068431

Methods and systems for in silico experimental designing and performing a biological workflow

Embodiments describe a non-transitory computer-readable storage medium encoded with instructions, executable by a processor, comprising instructions for a method for performing a biological workflow in silico comprising: presenting to a user a plurality of subroutines listed in a sequential order of the workflow, wherein at least two subroutines comprise two steps; providing the user ability to navigate to any subroutine and/or step, to select a subroutine, to view, set, or change one or more parameters associated with step/subroutine; providing option to display biomolecule(s) resulting from execution of the subroutines/steps; and providing an option to navigate to a prior subroutine and change a parameter of a step of the prior subroutine and execute the step of the prior subroutine, if the user is not satisfied with the displayed biomolecule(s). Computer systems and methods for performing a biological workflow in silico are also described.. ... Life Technologies Corporation

03/09/17 / #20170067918

Composition and method for measuring thallium influx and efflux

The present invention relates to methods for detecting the activity of an ion channel in a cell. The methods comprise providing a loading buffer solution to a cell that has an ion channel. ... Life Technologies Corporation

03/09/17 / #20170067098

Clonal amplification of nucleic acid on solid surface with template walking

Novel methods of generating a localized population of immobilized clonal amplicons on a support are provided.. . ... Life Technologies Corporation

03/09/17 / #20170066642

Fluid interface to receive removable container

A fluidic interconnect includes a first interface including a liquid port, a gas port, and a cradle; a second interface including a liquid port, a gas port, and a swing bar to engage the cradle, a weight of a container attached to one of the first or second interfaces to drive the liquid port of the first interface into connection with the liquid port of the second interface and the gas port of the first interface into connection with the gas port of the second interface.. . ... Life Technologies Corporation

03/09/17 / #20170065981

Clamp devices for receiving a cartridge and methods for using same

A clamp device includes a frame; a carriage slidably engaged with the frame and including a receptacle to receive a cartridge including fluidics ports and electronic contacts, the carriage slidable between an open position and a closed position; a fluidics interface to engage the fluidics ports of the cartridge when the carriage is in a closed position; an electronic interface to engage the electronic contacts of the cartridge when the carriage is in the closed position; a driver to draw the fluidics interface and the electronic interface together, the cartridge secured between the electronic interface and the fluidics interface; and a sensor system to detect the presence of the cartridge in the receptacle and configured to prevent the driver from drawing the fluidics interface and electronic interface together when the cartridge is absent.. . ... Life Technologies Corporation

03/02/17 / #20170059577

Homogenous populations of molecules

The invention provides populations of molecules that are prepared as, or treated to become, homogeneous for one or more molecular characteristics. In an aspect, the invention relates to molecular weight standards that may be used to determine the molecular weight or apparent molecular weight of uncharacterized molecules, such as proteins and nucleic acids, as well as in other applications. ... Life Technologies Corporation

03/02/17 / #20170059517

Deep microwell designs and methods of making the same

An apparatus includes a substrate; a gate structure disposed over the substrate and having an upper surface; a well structure disposed over the substrate and defining a well over the upper surface of the gate structure; a conductive layer disposed on the upper surface of the gate structure and at least partially extending along a wall of the well in the well structure; and a dielectric structure disposed over the well structure and defining an opening to the well.. . ... Life Technologies Corporation

03/02/17 / #20170059516

Temperature control of chemical detection system

An apparatus for detecting chemical reactions may be provided. The apparatus may comprise a chemical detection device. ... Life Technologies Corporation

03/02/17 / #20170058256

Cell culture medium comprising small peptides

Cell culture media, concentrated media and feeds, methods of manufacturing cell culture media and feeds, and methods of culturing cells are provided. One or more small peptides, including dipeptides are added to the cell culture media to provide improved stability and improved conditions for culturing cells.. ... Life Technologies Corporation

03/02/17 / #20170056881

Fluidics system for sequential delivery of reagents

The invention provides a passive fluidics circuit for directing different fluids to a common volume, such as a reaction chamber or flow cell, without intermixing or cross contamination. The direction and rate of flow through junctions, nodes and passages of the fluidics circuit are controlled by the states of upstream valves (e.g. ... Life Technologies Corporation

02/23/17 / #20170051345

Method of determining the nucleotide sequence of oligonucleotides and dna molecules

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of dna polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a dna fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. ... Life Technologies Corporation

02/23/17 / #20170051344

Purification chemistries and formats for sanger dna sequencing reactions on a micro-fluidics device

According to various embodiments described herein, a microfiuidics-chip based purification device and system for sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. ... Life Technologies Corporation

02/23/17 / #20170051342

Chemically-enhanced primer compositions, methods and kits

A composition is provided comprising a negatively charged group, an oligonucleotide sequence and at least none or one nuclease-resistant linkage group to form a chemically-enhanced primer. The chemically-enhanced primer can be used for sequencing and fragment analysis. ... Life Technologies Corporation

02/16/17 / #20170046308

Robust detection of variablility in multiple sets of data

. . The present teachings comprise systems and methods for calibrating the background or baseline signal in a pcr or other reaction. The background signal derived from detected emissions of sample wells can be subjected to a normalized statistical metric, and be compared to a threshold or other standard to discard outlier cycles or other extraneous data. ... Life Technologies Corporation

02/16/17 / #20170045496

Carbopyronone compounds useful as diagnostic adjuvants

Described herein are fluorescent compounds and methods and comprising these compounds. The compounds disclosed herein are carbopyronine reagents that fluoresce in the red portion of the uv/vis spectrum and provide bright fluorescence intensity, uniform cell staining, and good retention within live cells as well as low toxicity toward cells.. ... Life Technologies Corporation

02/16/17 / #20170045421

Devices and methods for laser capture microdissection

A carrier for holding a biological sample includes a substrate. The substrate is configured to engage a first sample chamber comprising a first opening characterized by a first opening diameter or a second sample chamber comprising a second opening characterized by a second opening diameter that is greater than the first opening diameter. ... Life Technologies Corporation

02/16/17 / #20170044602

Phase-preotecting reagent flow ordering for use in sequencing-by-synthesis

A system and machine readable medium for nucleic acid sequencing includes disposing template polynucleotide strands in defined spaces disposed on a sensor array, at least some of the template polynucleotide strands having a sequencing primer and a polymerase operably bound therewith; exposing the template polynucleotide strands to a series of flows of nucleotide species flowed according to a predetermined ordering; and determining, for each of the series of flows of nucleotide species, how many nucleotide incorporations occurred for that particular flow to determine a predicted sequence of nucleotides corresponding to the template polynucleotide strands, wherein the predetermined ordering (a) is not a series of consecutive repetitions of a 4-flow permutation of four different nucleotide species, (b) is not specifically tailored to a particular combination of a particular template polynucleotide strand to be sequenced and a particular sequencing primer to be used, and (c) comprises a phase-protecting flow ordering.. . ... Life Technologies Corporation

02/16/17 / #20170044506

Cdna synthesis using a reversibly inactivated reverse transcriptase

The present invention provides compositions and methods for a reverse transcription reaction using a reversibly inactivated reverse transcriptase enzyme. The reversibly inactivated reverse transcriptase enzyme results from a chemical modification which inactivates the reverse transcriptase enzyme. ... Life Technologies Corporation

02/09/17 / #20170038398

Long wavelength fluorogenic intracellular ion indicators that are well retained in the cytosol

Cell permeable metal ion indicator compounds and methods of their use and synthesis are described. The compound comprises a metal chelating moiety (mc), a reporter molecule and two or more lipophilic groups (gl) covalently bonded through a linker to the reporter molecule, wherein the lipophilic groups, when present in a live cell, are cleaved resulting in two or more negatively charged groups.. ... Life Technologies Corporation

02/09/17 / #20170038334

Electric field directed loading of microwell array

An apparatus includes a device substrate including an array of sensors. Each sensor of the array of sensors can include a electrode structure disposed at a surface of the device substrate. ... Life Technologies Corporation

02/09/17 / #20170038288

Particle analyzing systems and methods using acoustic radiation pressure

The present invention comprises methods and systems that use acoustic radiation pressure.. . ... Life Technologies Corporation

02/09/17 / #20170037458

Multi-component inhibitors of nucleic acid polymerases

The present invention provides multi-component inhibitors of nucleic acid polymerases, methods of making, and methods of using same. One component of the multi-component inhibitor is a molecule that binds to a polymerase (i.e., a polymerase-binding molecule (pbm)), but does not thereby substantially inhibit its polymerase activity. ... Life Technologies Corporation

02/09/17 / #20170037248

Fluorinated resorufin compounds and their application

The invention provides novel fluorinated resorufin compounds that are of use in a variety of assay formats. Also provided are methods of using the compounds and kits that include a compound of the invention and instructions detailing the use of the compound in one or more assay formats.. ... Life Technologies Corporation

02/02/17 / #20170030854

Sensor for chemical analysis and methods for manufacturing the same

Provided herein is a sensor comprising a substrate having a first reaction region and a second reaction region, a first electrode associated with the first reaction region, a second electrode associated with the second reaction region and a third electrode wherein the third electrode is common to both the first reaction region and the second reaction region.. . ... Life Technologies Corporation

02/02/17 / #20170029874

Methods of using fet labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same

Methods and compositions are provided for detecting a primer extension product in a reaction mixture. In the subject methods, a primer extension reaction is conducted in the presence of a polymerase having 3′→5′ exonuclease activity and at least one fet labeled oligonucleotide probe that includes a 3′→5′ exonuclease resistant quencher domain. ... Life Technologies Corporation

02/02/17 / #20170029873

Methods and apparatus for detecting molecular interactions using fet arrays

Methods and apparatuses relating to large scale fet arrays for analyte detection and measurement are provided. Chemfet (e.g., isfet) arrays may be fabricated using conventional cmos processing techniques based on improved fet pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. ... Life Technologies Corporation

02/02/17 / #20170029298

Electrochemical decontamination cells

Contaminants are filtered from a fluid flow stream and the filter is regenerated by a process including steps of: providing a filter material comprising both carbon and potassium iodide; passing a contaminated fluid stream in contact with the filter material; adsorbing contaminants from the fluid stream onto surfaces in the filter material; passing an electric current through the filter material with adsorbed contaminant thereon; disassociating contaminant from the surfaces of the filter material; and removing disassociated contaminant from the filter material by carrying away the disassociated contaminant in a fluid flow mass.. . ... Life Technologies Corporation

02/02/17 / #20170029144

Fluid dispensing system with rotatable port assembly

A fluid dispensing system includes a manifold port assembly having a housing with an inlet port and a plurality of outlet ports formed thereon and a carousel bounding a plurality of spaced apart fluid paths that each extend therethrough from a corresponding first end to an opposing corresponding second end, at least a portion of at least one of the fluid paths being completely radially encircled by the carousel at a spaced apart location from the housing. A fluid source is fluid coupled with the inlet port of the manifold port assembly. ... Life Technologies Corporation

01/26/17 / #20170025930

Rotor assembly including a housing for a sensor array component and methods for using same

A rotor assembly includes a rotor plate to rotate around a first axis, a bucket attached to the rotor plate and to rotate around a second axis, and a stop plate to rotate around the first axis between an open position and a closed position. When in the closed position, the stop plate engages the bucket to fix an angular position of the bucket relative to a plane of rotation of the rotor assembly. ... Life Technologies Corporation

01/26/17 / #20170022552

Methods and systems for quantification without standard curves

A method for quantitation of biological material in a biological sample is provided. The method includes receiving amplification data from amplification of a first and a second reference sample and receiving amplification data from amplification of a biological sample. ... Life Technologies Corporation

01/19/17 / #20170016853

Optical system for capillary electrophoresis

A system for conducting a capillary electrophoresis assay includes a light source, an interface, and an illumination optical system. The light source is configured to provide a source beam of electromagnetic radiation. ... Life Technologies Corporation

01/19/17 / #20170016043

System and method for improved transient protein expression in cho cells

The present invention is directed generally to systems and methods suitable for high level expression of recombinant proteins in suspension cho cells. In particular, the invention allows introduction of the invention obviates the need to replace, replenish or supplement the growth medium during the procedure. ... Life Technologies Corporation

01/19/17 / #20170014784

System and methods for making and processing emulsions

An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. ... Life Technologies Corporation

01/12/17 / #20170011165

Visualization tool for qpcr genotyping data

Systems and methods are used to display data obtained from a qpcr instrument. Each of two or more samples is probed with a first labeling probe and a second labeling probe. ... Life Technologies Corporation

01/12/17 / #20170009275

Substrates and methods useful in sequencing

A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.. ... Life Technologies Corporation

01/12/17 / #20170009232

Compositions and methods for inhibition of nucleic acids function

The invention relates generally to compositions and methods for inhibiting the function of target nucleic acids by sequence specific binding. The compositions and methods can be used for inhibition of micro rnas and other relatively short non-coding rnas.. ... Life Technologies Corporation

01/12/17 / #20170007977

Method of distributing discrete polymer networks

A method of preparing a discrete polymer network array include mixing a plurality of nucleic acid polymer networks with a plurality of color-activated polymer networks to form a dispersion, applying the dispersion to an array of wells, the nucleic acid polymer networks selectively depositing into wells of the array of wells, and rinsing the array of wells to selectively remove the plurality of color-activated polymer networks.. . ... Life Technologies Corporation

01/05/17 / #20170002410

Conjugation of carboxyl functional hydrophilic beads

A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.. ... Life Technologies Corporation

01/05/17 / #20170002401

Compositions, kits and methods for synthesis and/or detection of nucleic acids

A composition comprising a thermostable dna polymerase; and a pcr inhibitor blocking agent, wherein the pcr inhibitor blocking agent is present in an amount effective to enhance tolerance of an assembled pcr to a pcr inhibitor.. . ... Life Technologies Corporation

01/05/17 / #20170002152

Polymer substrates formed from carboxy functional acrylamide

A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the oh of the carboxyl group. ... Life Technologies Corporation

01/05/17 / #20170001160

Gas spargers and related container systems

A container system includes a bag comprised of one or more sheets of flexible polymeric material, the bag having an interior surface at least partially bounding a chamber, the chamber being adapted to hold a fluid. A flexible sparging sheet is secured to the interior surface of the bag so that a compartment is formed between the interior surface of the bag and the sparging sheet, at least a portion of the sparging sheet allowing gas to pass therethrough. ... Life Technologies Corporation








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