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New England Biolabs Inc patents


Recent patent applications related to New England Biolabs Inc. New England Biolabs Inc is listed as an Agent/Assignee. Note: New England Biolabs Inc may have other listings under different names/spellings. We're not affiliated with New England Biolabs Inc, we're just tracking patents.

ARCHIVE: New 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 | Company Directory "N" | New England Biolabs Inc-related inventors


Synthon formation

This disclosure provides, among other things, a composition comprising: a 5′ exonuclease; a strand-displacing polymerase; and optionally a single strand dna binding protein and/or a ligase. A method for polynucleotide assembly to form a synthon, as well as a kit for performing the same, are also described.. ... New England Biolabs Inc

Methods for labeling a population of rna molecules

A method of labeling, and optionally enriching, for a population of target rna molecules in a mixture of rnas is provided. In some embodiments, the method may comprise (a) adding a label to the 5′ end of 5′-diphosphorylated or 5′-triphosphorylated target rna molecules in a sample by incubating the sample with labeled gtp and a capping enzyme; and (b) optionally enriching for target rna comprising the affinity tag-labeled gmp using an affinity matrix that binds to the affinity tag. ... New England Biolabs Inc

Helicase suppression of non-template amplification

Provided herein is a method for reducing amplification of non-template molecules in a nucleic acid sample. In certain embodiments, the method involves adding a helicase to a reaction mixture for non-helicase-dependent amplification of target nucleic acid.. ... New England Biolabs Inc

Compositions and methods for analyzing modified nucleotides

Methods and compositions are provided for identifying any of the presence, location and phasing of methylated and/or hydroxymethylated cytosines in nucleic acids including long stretches of dna. In some embodiments, the method may comprise reacting a first portion (aliquot) of a nucleic acid sample with a dioxygenase and optionally a glucosyltransferase in a reaction mixture containing the nucleic acid followed by a reaction with a cytidine deaminase to detect and optionally map 5mc in a dna. ... New England Biolabs Inc

High fidelity restriction endonucleases

Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. ... New England Biolabs Inc

High fidelity restriction endonucleases

Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (fi) in a specified buffer that is greater than the fi of the non-mutated enzyme in the same buffer.. . ... New England Biolabs Inc

Mutant reverse transcriptase

A mutant mmlv reverse transcriptase that may have an improvement in one or more properties is provided. For example, the present reverse transcriptase is believed to be more efficient relative to other commercially available mmlv reverse transcriptase variants, particularly for templates with a higher gc content.. ... New England Biolabs Inc

Method for reducing sequencing errors caused by dna fragmentation

Provided herein, among other things, is a method for reducing sequencing errors caused by mechanical dna fragmentation. In some embodiments, this method may comprise: (a) obtaining a dna sample; (b) mechanically fragmenting the dna sample to produce a template; (c) treating the fragmented dna with a plurality of dna repair enzymes; and (d) obtaining a sequence of the fragmented dna. ... New England Biolabs Inc

Methods and compositions for preventing concatemerization during template-switching

Compositions and methods for performing a template-switching reaction are provided that may include reducing or eliminating concatemerization of the template-switching oligonucleotide (tso). In some embodiments, the composition may comprise: a reverse transcriptase; a tso that includes a recognition sequence for a site-specific double strand nucleic acid cleaving enzyme, wherein the tso has at its 3′ end at least one nucleotide capable of hybridizing to at least one or more non-templated nucleotides added to a templated cdna strand by the reverse transcriptase; and a site-specific double strand nucleic acid cleaving enzyme that cleaves the tso at the recognition sequence.. ... New England Biolabs Inc

Enrichment and sequencing of rna species

Provided herein is a method for making an cdna library, comprising adding an affinity tag-labeled gmp to the 5′ end of targeted rna species in a sample by optionally decapping followed by incubating the sample with an affinity tag-labeled gtp and a capping enzyme, enriching for rna comprising the affinity tag-labeled gmp using an affinity matrix that binds to the affinity tag, reverse transcribing the enriched rna to produce a population of cdnas, and adding a tail to the 3′ end of the population of cdnas using a terminal transferase, to produce an cdna library.. . ... New England Biolabs Inc

Compositions and methods relating to synthetic rna polynucleotides created from synthetic dna oligonucleotides

Compositions and methods are provided for forming a single rna polynucleotide from a plurality of dna oligonucleotides in a single reaction chamber using combined reagents in a single step reaction. Dna polymerase, rna polymerase and single stranded (ss) dna oligonucleotides are combined where each dna oligonucleotide has one or more sequence modules, wherein one sequence module in the first ss dna oligonucleotide is complementary to a sequence module at the 3′ end of the second ss dna oligonucleotide; and wherein a second module on the first ss dna oligonucleotide is an rna polymerase promoter sequence; and forming a single rna polynucleotide, excluding the rna promoter sequence, derived from the first and second dna oligonucleotides. ... New England Biolabs Inc

Methods for enriching for a population of rna molecules

A method of enriching for a population of rna molecules in a mixture of rnas is provided. In some embodiments, the method may comprise (a) adding an affinity tag to the 5′ end of 5′-diphosphorylated or 5′-triphosphorylated rna molecules in a sample by incubating the sample with an affinity tag-labeled gtp and a capping enzyme; and (b) enriching for rna comprising the affinity tag-labeled gmp using an affinity matrix that binds to the affinity tag.. ... New England Biolabs Inc

Thermostable variants of t7 rna polymerase

A bacteriophage rna polymerase variant is provided. In some embodiments, the variant may have increased thermostability relative to the corresponding wild type bacteriophage rna polymerase and/or wild type t7 rna polymerase. ... New England Biolabs Inc

Compositions and methods for analyzing modified nucleotides

A method for identifying any of the presence, location and phasing of modified cytosines (c) in long stretches of nucleic acids is provided. In some embodiments, the method may comprise (a) reacting a first portion of a nucleic acid sample containing at least one c and/or at least one modified c with a dna glucosyltransferase and a cytidine deaminase to produce a first product and/or reacting a second portion of the sample with a dioxygenase, optionally a dna glucosyltransferase and a cytidine deaminase to produce a second product and; (b) comparing the sequences from the first and optionally the second product obtained in (a), or amplification products thereof, with each other and/or an untreated reference sequence to determine which cs in the initial nucleic acid fragment are modified. ... New England Biolabs Inc

07/13/17 / #20170198250

Compositions and methods for extending storage time of competent cells at -20°c

Compositions and methods are provided for storing prokaryotic cells including competent prokaryotic cells at −20° c. In a buffer so that the cells are suitable for transformation at 0° c. ... New England Biolabs Inc

06/01/17 / #20170152494

Fusion polymerase and method for using the same

This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a dna polymerase; and (b) a heterologous sequence-specific dna binding domain. A method for copying a dna template, as well as a kit for performing the same, are also described.. ... New England Biolabs Inc

06/01/17 / #20170152493

Fusion polymerase and method for using the same

This disclosure provides, among other things, a composition comprising: comprising a fusion protein comprising: (a) a dna polymerase; and (b) a heterologous sequence-specific dna binding domain. A method for copying a dna template, as well as a kit for performing the same, are also described.. ... New England Biolabs Inc

05/18/17 / #20170138959

Deglycosylation reagents and methods

Compositions and methods are provided for efficiently preparing a completely deglycosylated antibody where efficiency is measured in relative amounts of reagents in soluble or lyophilized form, and time and temperature of the reaction. Compositions and methods are also provided for separating substantially all n-linked glycans from a glycosylated antibody and for preserving functionality of the antibody. ... New England Biolabs Inc

05/11/17 / #20170128554

Universal n-glycan binding reagent

Methods of capturing n-glycan linked glycomolecules including n-glycans, n-glycopeptides and n-glycoproteins are described. The methods provide substantially unbiased capture of charged and uncharged n-glycans and/or n-glycan linked glycomolecules. ... New England Biolabs Inc








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