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Qiagen Gmbh patents


Recent patent applications related to Qiagen Gmbh. Qiagen Gmbh is listed as an Agent/Assignee. Note: Qiagen Gmbh may have other listings under different names/spellings. We're not affiliated with Qiagen Gmbh, we're just tracking patents.

ARCHIVE: New 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 | Company Directory "Q" | Qiagen Gmbh-related inventors


Method for isolating poly(a) nucleic acids

The present invention pertains inter alia to a method for isolating poly(a) nucleic acids having a single stranded poly(a) stretch from a nucleic acid containing sample comprising: (a) providing a hybridization composition comprising: i) a nucleic acid containing sample; ii) a hybridization solution comprising: aa. A sodium salt; bb. ... Qiagen Gmbh

Method for isolating extracellular nucleic acids using anion exchange particles

The present invention pertains to methods and kits for isolating extracellular nucleic acids from a biological sample using anion exchange particles. It was found that incorporating into the binding mixture a polyoxyalkylene fatty alcohol ether compensates performance variations that are attributable to differences in the anion exchange surface as they may occur e.g. ... Qiagen Gmbh

Antioxidant compounds for cleave formulations that support long reads in sequencing-by-synthesis

. . Methods, compositions, devices, systems and kits are described including, without limitation, reagents and mixtures for determining the identity of nucleic acids in nucleotide sequences using, for example, sequencing by synthesis methods. Higher base reads with lower error rates are achieved with the use of ascorbic acid (aa), also known as vitamin c, as an antioxidant additive to the cleave reagent.. ... Qiagen Gmbh

Efficiency improving ligation methods

The present invention provides new methods and kits to improve the efficiency of ligation reactions, in particular in molecular biology applications, such as the next generation sequencing (ngs) library construction methods. In next-generation sequencing methods, the ligation step is critical in adding sequencing platform-specific adapters to the dna fragments that are to be sequenced. ... Qiagen Gmbh

Method, lysis solution and kit for selectively depleting animal nucleic acids in a sample

The present invention relates to a method for selectively depleting animal nucleic acids from non-animal nucleic acids in a sample, which comprises animal cells and at least one further type of cells, selected from microbial cells and plant cells or a combination thereof, to a lysis solution a to be used in and to a kit to carry out said method as well as to the use of a particular silica membrane as both, a filtration matrix for separating essentially intact microbial and/or plant cells from lysed animal cells and an adsorption matrix for nucleic acids, in particular in a method according to the present invention.. . ... Qiagen Gmbh

Enhancing sequencing performance in sequencing-by-synthesis

The invention relates to methods, compositions, devices, systems and kits as described including, without limitation, reagents and mixtures for determining the identity of nucleic acids in nucleotide sequences using, for example, sequencing by synthesis (sbs) methods. In particular, the present invention contemplates the use of chelators in washing reagents to improve sbs performance.. ... Qiagen Gmbh

Photoprotective mixtures as imaging reagents in sequencing-by-synthesis

The invention relates to methods, compositions, devices, systems and kits as described including, without limitation, reagents and mixtures for determining the identity of nucleic acids in nucleotide sequences using, for example, sequencing by synthesis methods. In particular, the present invention contemplates the use of photoprotective mixture of compounds as imaging reagents to improve stability and storage of fluorescent compounds, including but not limited to, nucleotides with fluorescent labels.. ... Qiagen Gmbh

Method for immobilizing a nucleic acid molecule on a solid support

The present invention is directed to a method for immobilizing a nucleic acid molecule on a solid support and to a use of a combination of a first nucleic acid immobilized primer linked to a solid support and a second immobilized primer linked to said solid support in said method.. . ... Qiagen Gmbh

Method for hybridizing a nucleic acid molecule

. . The present invention is directed to a method for hybridizing a nucleic acid molecule to another nucleic acid molecule and to a use of a nucleic acid molecule to reduce and/or avoid the formation of hairpin structures of a nucleic acid target molecule.. . ... Qiagen Gmbh

Preparation of adapter-ligated amplicons

The present invention is directed to novel methods and kits to be employed for preparing adapter-ligated amplicons or a sequencing library of a target dna, respectively.. . ... Qiagen Gmbh

High multiplex pcr with molecular barcoding

The present disclosure provides methods and kits for performing high multiplex pcr using molecular barcodes. The methods disclosed herein separately extend a set of primers (bc primers) that each comprise a target-specific sequence, a molecular barcode and a universal sequence, and amplify the resulting extension products using another set of primers (la primers) that each comprise another target-specific sequence and a universal sequence. ... Qiagen Gmbh

Highly specific and sensitive methods for detecting enterohemorrhagic escherichia coli serotypes o157:h7 and/or o145:h28

The present invention relates to methods and kits for the specific detection of escherichia coli (e.coli) serotypes o157:h7 and/or o145:h28. The methods and kits are based on the values for the e. ... Qiagen Gmbh

Methods for rt-pcr comprising an anionic polymer

The present invention is directed to methods for the generation of nucleic acids from an rna template and further nucleic acid replication. Specifically, the invention is directed to the generation and amplification of nucleic acids by reverse transcriptase-polymerase chain reaction (rt-pcr).. ... Qiagen Gmbh

Peptide composition and uses thereof

Subject of the invention is a composition comprising at least one fragment of the peptide esat-6 and at least one fragment of the peptide cfp-10. Preferably, the fragments comprise at least two sets of peptides, a first set comprising at least one peptide of from about 7 to 14 amino acid residues in length and a second set comprising at least one peptide of from 16 amino acid residues or greater. ... Qiagen Gmbh

10/05/17 / #20170283869

Preparation of adapter-ligated amplicons

The present invention is directed to novel methods and kits to be employed for preparing adapter-ligated amplicons or a sequencing library of a target dna, respectively.. . ... Qiagen Gmbh

09/14/17 / #20170259262

Laminar fluidic separation in flowcells for minimal reagent usage

A processing instrument flowcell having a flowcell channel with an upstream channel end, a downstream channel end, a longitudinal axis extending from the upstream channel end to the downstream channel end, and a first operative surface extending between the upstream channel end and the downstream channel end and configured to receive a first number of dna templates. A first reagent inlet is fluidically connected to the upstream channel end at a location adjacent the first operative surface. ... Qiagen Gmbh

08/17/17 / #20170233805

Additives to improve sequencing by synthesis performance

The invention relates to methods, compositions, devices, systems and kits are described including, without limitation, reagents and mixtures, for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods.. . ... Qiagen Gmbh

08/17/17 / #20170233796

Polyphenolic additives in sequencing by synthesis

The invention relates to methods, compositions, devices, systems and kits as described including, without limitation, reagents and mixtures for determining the identity of nucleic acids in nucleotide sequences using, for example, sequencing by synthesis methods. In particular, the present invention contemplates the use of polyphenolic compounds, known as antioxidant additives, to improve the efficiency of sequencing-by-synthesis reactions. ... Qiagen Gmbh

08/17/17 / #20170233725

Scavenger compounds for improved sequencing-by-synthesis

The present invention discloses methods of applications of indole-3-propionic acid, l-carnitine and o-acetyl-l-carnitine in one or more different reactive steps of a sequencing-by-synthesis workflow. The reactive steps employing these compounds include, but are not limited to, cleaving, imaging, incorporating bases and washing. ... Qiagen Gmbh

07/13/17 / #20170198279

Method for isolating rna with high yield

A phenol-free method for isolating a nucleic acid from a sample is provided, said method comprising the following steps: a) preparing a precipitation mixture by adding at least one metal cation precipitant and at least one organic solvent selected from aprotic polar solvents and protic solvents to the sample, wherein the precipitation mixture i) comprises the metal cation precipitant; ii) comprises the organic solvent in a concentration of 15% or less; iii) comprises a buffering agent; and iv) has an acidic ph value, and precipitating proteins; b) separating the precipitate from the supernatant, wherein the supernatant comprises small rna having a length of less than 200 nt and large rna having a length of at least 1000 nt; and c) isolating a nucleic acid from the supernatant. Using an organic solvent as claimed during the protein precipitation step in the defined concentration provides a supernatant which in addition to small rna also comprises large rna. ... Qiagen Gmbh

06/29/17 / #20170182494

Flowcells with microretainers and particle separators for discrete seeding microspots

A flowcell for a sequencing instrument. The flowcell includes a fluid inlet, a fluid outlet, a flow channel formed between an at least partially transparent cover and a base and fluidly connecting the fluid inlet to the fluid outlet, and a capture substrate provided in the flow channel. ... Qiagen Gmbh

06/29/17 / #20170182493

Thin-film flowcells

A sequencing instrument including a sequencing stage and a microscope. The sequencing stage has a reference plate with a flat reference surface. ... Qiagen Gmbh

06/08/17 / #20170159042

Lysis, binding and/or wash reagent for isolating and/or purifying nucleic acids

The present invention relates to a lysis, binding and/or wash reagent for isolating and/or purifying nucleic acids and a method for isolating and/or purifying nucleic acids.. . ... Qiagen Gmbh

06/08/17 / #20170159041

Lysis, binding and/or wash reagent for isolating and/or purifying nucleic acids

The present invention relates to a lysis, binding and/or wash reagent for isolating and/or purifying nucleic acids and a method for isolating and/or purifying nucleic acids.. . ... Qiagen Gmbh

03/02/17 / #20170058343

Systems and methods for color detection in high-throughput nucleic acid sequencing systems

A sequencing instrument optical system having a combined light source with multiple collinear excitation beams having different respective excitation wavelengths, a sequencing surface having dna templates and nucleobase labels configured to emit a respective emission light at a different respective emission wavelength upon excitation by one or more of the excitation beams, a color camera configured to detect the emission light of each of the nucleobase labels, a first optical pathway configured to direct the collinear excitation beams from the combined light source to the sequencing surface, and a second optical pathway configured to direct the emission light from the sequencing surface to the color camera.. . ... Qiagen Gmbh

02/23/17 / #20170052095

Fixative composition for cell-comprising liquid samples

The present invention refers to a formal-in-free fixative composition, suitable for the fixation of cells in particular in liquid samples, the use of said fixative for the treatment of biological samples, a method for the treatment of cell-comprising liquid samples, a kit comprising said fixative and a method for diagnosis of cell-comprising biological material samples.. . ... Qiagen Gmbh

02/02/17 / #20170029880

Optimization of dna amplification reactions

The present invention relates to methods and uses where a combination of (i) an enzyme exhibiting 5′->3′ exonuclease activity and specifically degrading 5′-phosphorylated strands of double-stranded nucleic acids is used, and (ii) one or more primer pair(s), each primer being 5′ phosphorylated, in a method comprising at least a first and a second amplification reaction of a first and a second template, respectively, for preventing contamination of the second amplification reaction with amplification products of the first amplification reaction, as well as novel methods and kits using the combination. Also, the present invention relates to kits comprising the enzyme and either 5′-phosphorylated primers or a polynucleotide kinase.. ... Qiagen Gmbh

01/26/17 / #20170022555

Modular flow cells and methods of sequencing

Modular flow cells, devices with modular flow cells, and methods of sequencing using modular flow cells, as well as systems and kits including modular flow cells, are described, permitting sequencing wherein less than the full capacity for sequencing is desired.. . ... Qiagen Gmbh

01/12/17 / #20170009294

Method of amplification of a short tandem repeat locus

Short tandem repeat (str) markers are genetic elements that are frequently used in the fields of forensic analysis, paternity determination and detection of genetic diseases and cancers. Such analysis involves the amplification of str loci. ... Qiagen Gmbh

01/05/17 / #20170002398

Nucleic acid amplification method

. . The invention relates to a method for the simultaneous detection of at least four nucleic acids labeled with a covalently attached dye in a sample comprising the step of detecting the fluorescence emission of said fluorescent dyes upon excitation, wherein the at least four dyes attached to said nucleic acids are selected from the groups of i) 6-fam or 5-fam or a blend thereof covalently attached to a first nucleic acid, ii) dy-530, hex or atto 532 or a blend thereof covalently attached to a second nucleic acid, iii) att0550 or dy-555 or a blend thereof covalently attached to a third nucleic acid, iv) att0565, dy510-xl or rox or a blend thereof covalently attached to a fourth nucleic acid, v) dy632 or dy520-xl or a blend thereof covalently attached to a fifth nucleic acid and, vi) chromeo 494 attached to a sixth nucleic acid, wherein chromeo 494 must be one of the four selected.. . ... Qiagen Gmbh








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